Lipolytic enzymes of Trichophyton rubrum
TL;DR: Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.
Abstract: Trichophyton rubrum cells contain lipase, phospholipases A and B and acyl CoA lysolecithin acyl transferase activities. This dermatophyte excretes lipase and phospholipase A into the growth medium when cultivated in Sabouraud's broth. Extracellular lipase has optimum activity at pH 8.0 whereas the intracellular lipase is maximally active at pH 8.0 whereas the intracellular lipase is maximally active at pH 7.0. The optimum pH of phospholipase A and B activities which are localized in 15000 g sedimentable cell fragments are 7.0 and 6.0 respectively. Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.
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TL;DR: The known mechanisms and factors involved in human tinea and important for the host response are briefly delineated and Cellular defence mechanisms appear to be decisive for clearing of infection.
Abstract: Skin infection caused by dermatophytes is called tinea. In this short review, the known mechanisms and factors involved in human tinea and important for the host response are briefly delineated. To establish tinea, fungal propagules must attach to the skin, germinate and overcome the epidermal barrier. Keratinases and other enzymes are released in this process and host keratinocytes are activated. This is followed by an inflammatory response mediated by a plentitude of cytokines and receptors, comprising innate as well as acquired immunity, including neutrophilic granulocytes, macrophages, antibodies and T cells. Cellular defence mechanisms appear to be decisive for clearing of infection. Nails and hair follicles are the particular sites often invaded by dermatophytes that show distinctive patterns of infection. Nails are largely excluded from defence mechanisms and steroid hormones of the pilosebaceous units may have a particular effect on follicular infection. Fungal invasion of the dermis can cause granulomatous reactions. Immune reactions to dermatophytes may lead to sterile eruptions distant from the infected skin areas.
41 citations
TL;DR: The susceptibilities of 77 dermatophytes to miltefosine, 1,12-bis(4-pentylpyridinium)dodecane, PYR, itraconazole, terbinafine (TRB), and butenafine (BTF) were compared and MI was more active than ITC.
Abstract: The susceptibilities of 77 dermatophytes to miltefosine (MI), 1,12-bis(4-pentylpyridinium)dodecane (PYR), 1,12-bis(tributylammonium)dodecane (AM), itraconazole (ITC), terbinafine (TRB), and butenafine (BTF) were compared. Geometric mean MICs of TRB, BTF, ITC, MI, PYR, and AM were 0.039, 0.059, 1.718, 0.671, 6.006, and 4.771 μg/ml, respectively. MI was more active than ITC (P < 0.001).
38 citations
Cites background from "Lipolytic enzymes of Trichophyton r..."
...Since dermatophytes also produce phospholipase(s) (3, 15), we compared the in vitro activities of MI and two biscationic salts (Fig....
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TL;DR: The experiments showed that short chain saturated fatty acids (C 7:0‐C 11:0) are more toxic to dermatophytes than long chain ones (> C 12:0), and the antifungal activity decreases with increasing carbon chain length in the even‐numbered carbon chain series.
Abstract: Ten saturated and three unsaturated fatty acids over a wide spectrum of their carbon chain length were tested for their fungitoxicity against five dermatophytes. The experiments showed that short chain saturated fatty acids (C 7:0-C 11:0) are more toxic to dermatophytes than long chain ones (> C 12:0). The antifungal activity decreases with increasing carbon chain length in the even-numbered carbon chain series. Odd-numbered carbon fatty acids show irregularities in these respects. Undecanoic acid (C 11:0) is outstandingly most toxic in the C 7:0-C 18:0 series. Polyunsaturated fatty acids are more toxic than their corresponding saturated acids. The per cent growth inhibition decreased from day 4 to day 12 at all concentrations suggesting the degradation and/or utilization of fatty acids by the fungi during the observation period. The results are discussed on the background of the biochemistry of fatty acids.
37 citations
TL;DR: The present review will bring out the differences or similarities of lipid metabolism in diverse fungal species and each section will deal, comparatively, with various aspects of fungal lipid metabolism and physiology.
Abstract: So far, reviews that have appeared on fungal lipids present data mainly on the lipid composition of these organisms and the influence of lipids on their physiology. These reviews provide little information about the enzymes of lipid metabolism in these organisms and it is assumed, by most workers, that lipid synthesis in all fungi takes place as in Saccharomyces cervesiae, the only fungus in which the complete pathways of phospholipid biosynthesis have been worked out. During the last few years, literature has accumulated on lipid metabolic enzymes of other fungi, as investigators became increasingly interested in this area of research. The present review, after an introduction, will be divided into different sections and each section will deal, comparatively, with various aspects of fungal lipid metabolism and physiology. This review will, therefore, bring out the differences or similarities of lipid metabolism in diverse fungal species.
37 citations
TL;DR: The ability of Trichophyton simii HN 50, isolated from the Ghana Bird Sanctuary, Bharatpur, India, to produce extracellular keratinase was studied and the enzyme showed ability to degrade all of the 3 keratin substrates.
Abstract: The ability of Trichophyton simii HN 50, isolated from the Ghana Bird Sanctuary, Bharatpur, India, to produce extracellular keratinase was studied. Enzyme was produced on a keratin salt broth medium at pH7 and a temperature of 28 ± 1 °C. Enzyme secretion was best at 15 days of incubation. Asparagine and keratin were repressive to enzyme yield in comparison to gelatin. No relationship was observed between enzyme release and biomass. Exogenous sugars suppressed keratinase production in descending order as follows: glucose > mannose > maltose > arabinose > fructose. The enzyme showed ability to degrade all of the 3 keratin substrates. Buffalow skin was best degraded in the absence of glucose while chicken feathers were the least degraded in its presence.
35 citations
Cites methods from "Lipolytic enzymes of Trichophyton r..."
...Biomass was determined by gravimetric assay as suggested by Das and Banerjee [3]....
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Journal Article•
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
289,852 citations
TL;DR: In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.
369 citations
TL;DR: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatids inositols, and the relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
Abstract: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatidyl inositol, phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine phosphatidyl glycerol and phosphatidic acid in the polar lipid fraction of its mycelia. The relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
23 citations
TL;DR: Among subcultured strains, it was found a decreased lipolytic activity in Microsporum species and an increased lipolyic activity in Epidermophyton and someTrichophyton species.
Abstract: Lipolytic activity of dermatophytes was tested by the method generally used forCandida lipolytica. Most of the freshly isolated strains ofMicrosporum canis, M. gypseum, Epidermophyton floccosum andTrichophyton mentagrophytes gave positive reactions, whereas, only few strains ofT. schoenleini, T. violaceum, T. megnini, T. rubrum andT. tonsurans yielded such reactions. Among subcultured strains, it was found a decreased lipolytic activity inMicrosporum species and an increased lipolytic activity inEpidermophyton and someTrichophyton species.
19 citations
TL;DR: Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer, and their conversions to phosphatidyl choline and phosph atidyl inositol are suspected.
Abstract: Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer. Synthesis and catabolism of phosphatidyl ethanolamine and phosphatidyl serine are most rapid; phosphatidyl choline and phosphatidyl inositol are metabolised rather slowly. Catabolism of phosphatidyl ethanolamine and phosphatidyl serine are uniform and their conversions to phosphatidyl choline and phosphatidyl inositol are suspected.
10 citations