Journal ArticleDOI
Lipolytic enzymes of Trichophyton rubrum
S.K. Das,A.B. Banerjee +1 more
Reads0
Chats0
TLDR
Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.Abstract:
Trichophyton rubrum cells contain lipase, phospholipases A and B and acyl CoA lysolecithin acyl transferase activities. This dermatophyte excretes lipase and phospholipase A into the growth medium when cultivated in Sabouraud's broth. Extracellular lipase has optimum activity at pH 8.0 whereas the intracellular lipase is maximally active at pH 8.0 whereas the intracellular lipase is maximally active at pH 7.0. The optimum pH of phospholipase A and B activities which are localized in 15000 g sedimentable cell fragments are 7.0 and 6.0 respectively. Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.read more
Citations
More filters
Journal ArticleDOI
Haemolytic activities of Trichophyton species
P. Schaufuss,U. Steller +1 more
TL;DR: This work investigates the haemolytic activity of four Trichophyton species: T. rubrum, T. mentagrophytes complex, Tequinum and T. verrucosum.
Journal ArticleDOI
Lipids of dermatophytes
TL;DR: Phosphatides and fatty acid content of Epidermophyton floccosum, Microsporum cookie and Trichophyton mentagrophytes during different phases of growth decreased with age, which was reflected in constituent major phosphatides.
Journal ArticleDOI
Enzyme release by Trichophyton rubrum depends on nutritional conditions
TL;DR: It is shown that the extracellular enzyme activity is dependent on the nutrients supplied, and alkaline phosphatase and N‐acetyl‐β‐glucosaminidase were detected in all cultures lacking glucose.
Journal ArticleDOI
Effect of undecanoic acid on the production of exocellular lipolytic and keratinolytic enzymes by undecanoic acid-sensitive and -resistant strains of Trichophyton rubrum
S.K. Das,A.B. Banerjee +1 more
TL;DR: No exocellular keratinase could be detected in the udar strain which had very low capacity for invasion of keratinized tissue.
Journal ArticleDOI
Extracellular enzyme activities by Basidiobolus and Conidiobolus isolates on solid media.
TL;DR: Fifty‐seven isolates of Basidiobolus from reptiles and amphibians, and 7 other obtained from the American Type Culture Collection (ATCC) in Maryland were studied for their extracellular enzyme activities on solid media, finding that all these isolates produced activeextracellular lipase and protease and failed to exhibit amylase, deoxyribonuclease and ribonucleasing activities.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A rapid chemical method for quantification of lipids separated by thin-layer chromatography.
TL;DR: In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.
Journal ArticleDOI
Phospholipids of Trichophyton rubrum.
S.K. Das,A.B. Banerjee +1 more
TL;DR: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatids inositols, and the relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
Journal ArticleDOI
Lipolytic activity of dermatophytes.
G. Nobre,Maria Paula Viegas +1 more
TL;DR: Among subcultured strains, it was found a decreased lipolytic activity in Microsporum species and an increased lipolyic activity in Epidermophyton and someTrichophyton species.
Journal ArticleDOI
Phospholipid turnover in Trichophyton rubrum
S.K. Das,A.B. Banerjee +1 more
TL;DR: Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer, and their conversions to phosphatidyl choline and phosph atidyl inositol are suspected.