Lipolytic enzymes of Trichophyton rubrum
TL;DR: Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.
Abstract: Trichophyton rubrum cells contain lipase, phospholipases A and B and acyl CoA lysolecithin acyl transferase activities. This dermatophyte excretes lipase and phospholipase A into the growth medium when cultivated in Sabouraud's broth. Extracellular lipase has optimum activity at pH 8.0 whereas the intracellular lipase is maximally active at pH 8.0 whereas the intracellular lipase is maximally active at pH 7.0. The optimum pH of phospholipase A and B activities which are localized in 15000 g sedimentable cell fragments are 7.0 and 6.0 respectively. Supernatant obtained after removal of 1,005,000 g sedimentable fragments from cell extract contains acyl CoA lysolecithin acyl transferase which requires ATP, CoA, Mg2+ and an unsaturated fatty acid for its activity.
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Dissertation•
01 Jan 2006
TL;DR: In this paper, the gelatinase, urease, amylase, lipase, phospholipase and DNase activities of a group of chromoblastomycosis agents constituted by strains of Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophara bantiana and Exophiala jeanselmei were analysed and compared.
Abstract: The gelatinase, urease, amylase, lipase, phospholipase and DNase activities of a group of chromoblastomycosis agents constituted by strains of Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala jeanselmei were analysed and compared. All strains presented urease, gelatinase and lipase activity. Phospholipase activity was detected only on five of six strains of F. pedrosoi analysed. Amylase and DNase activities were not detected on the strains studied. Lipase and phospholipase activities, quantified by the ratio between colony diameter and precipitation halo by substrate uptake, changed significantly during the analysed period (10 days), showing varied behaviour of the strains. Lipase and phospholipase activities showed the best potential as enzyme markers to differentiate chromoblastomycosis agents and to correlate with the pathogenicity of this infection.
1 citations
01 Jan 2000
TL;DR: It emerges from the work done so far that the pathways of lipid biosynthesis/degradation and the lipid composition in dermatophytes are similar to those in yeasts and other fungi.
Abstract: This review presents the extensive work carried out on lipid components of dermatophytes, their biosynthesis, turnover and regulation. It emerges from the work done so far that the pathways of lipid biosynthesis/degradation and the lipid composition in dermatophytes are similar to those in yeasts and other fungi. Second messengers (Ca 2§ cAMP) were demonstrated to have a regulatory role in phospholipid metabolism and they mainly act by stimulating Ca2*/CaM or cAMP dependent protein kinase(s). Both these kinases were purified and characterized in Microsporum gypseum. Further work is being carried out to elucidate the molecular mechanism of regulation of phospholipid metabolism by these second messengers.
01 Jan 2006
TL;DR: Lipase and phospholipase activities showed the best potential as enzyme markers to differentiate chromoblastomycosis agents and to correlate with the pathogenicity of this infection.
Abstract: The gelatinase, urease, amylase, lipase, phospholipase and DNase activities of a group of chromoblastomycosis agents constituted by strains of Fonsecaea pedrosoi, F. compacta, Phialophora verrucosa, Cladosporium carrionii, Cladophialophora bantiana and Exophiala jeanselmei were analysed and compared. All strains presented urease, gelatinase and lipase activity. Phospholipase activity was detected only on five of six strains of F. pedrosoi analysed. Amylase and DNase activities were not detected on the strains studied. Lipase and phospholipase activities, quantified by the ratio between colony diameter and precipitation halo by substrate uptake, changed significantly during the analysed period (10 days), showing varied behaviour of the strains. Lipase and phospholipase activities showed the best potential as enzyme markers to differentiate chromoblastomycosis agents and to correlate with the pathogenicity of this infection.
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Journal Article•
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
289,852 citations
TL;DR: In this article, thin-layer chromatography was used to quantify all lipid classes to a lower limit of 15 μg of lipid, where the reduction in absorbance at 350 mμ is proportional to the amount of lipid.
369 citations
TL;DR: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatids inositols, and the relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
Abstract: Trichophyton rubrum grown in Sabouraud's liquid medium contains phosphatidyl inositol, polyphosphatidyl inositol, phosphatidyl choline, phosphatidyl serine, phosphatidyl ethanolamine phosphatidyl glycerol and phosphatidic acid in the polar lipid fraction of its mycelia. The relative proportion of these components in total phospholipid fraction remains almost constant throughout the culture life of this fungus.
23 citations
TL;DR: Among subcultured strains, it was found a decreased lipolytic activity in Microsporum species and an increased lipolyic activity in Epidermophyton and someTrichophyton species.
Abstract: Lipolytic activity of dermatophytes was tested by the method generally used forCandida lipolytica. Most of the freshly isolated strains ofMicrosporum canis, M. gypseum, Epidermophyton floccosum andTrichophyton mentagrophytes gave positive reactions, whereas, only few strains ofT. schoenleini, T. violaceum, T. megnini, T. rubrum andT. tonsurans yielded such reactions. Among subcultured strains, it was found a decreased lipolytic activity inMicrosporum species and an increased lipolytic activity inEpidermophyton and someTrichophyton species.
19 citations
TL;DR: Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer, and their conversions to phosphatidyl choline and phosph atidyl inositol are suspected.
Abstract: Phospholipid synthesis, interconversion and breakdown in T. rubrum were followed by radioactive tracer. Synthesis and catabolism of phosphatidyl ethanolamine and phosphatidyl serine are most rapid; phosphatidyl choline and phosphatidyl inositol are metabolised rather slowly. Catabolism of phosphatidyl ethanolamine and phosphatidyl serine are uniform and their conversions to phosphatidyl choline and phosphatidyl inositol are suspected.
10 citations