Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium
Margherita Y. Turco,Lucy Gardner,Jasmine Hughes,Tereza Cindrova-Davies,Maria J Gomez,Lydia Farrell,Michael Hollinshead,Steven G.E. Marsh,Jan J. Brosens,Hilary O. D. Critchley,Benjamin D. Simons,Myriam Hemberger,Myriam Hemberger,Bon-Kyoung Koo,Ashley Moffett,Graham J. Burton +15 more
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TLDR
These organoids expand long-term, are genetically stable and differentiate following treatment with reproductive hormones, and provide a foundation to study common diseases, such as endometriosis and endometrial cancer, as well as the physiology of early gestation.Abstract:
Turco et al. derive long-term genetically stable organoids from normal endometrium and the decidua that recapitulate characteristics of in vivo uterine glands, respond to hormones and differentiate into secretory and ciliated endometrial cells.read more
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Organoids in cancer research
Jarno Drost,Hans Clevers +1 more
TL;DR: In this Review, Drost and Clevers discuss the recent advances in organoid models of cancer and how they can be exploited to drive the translation of basic cancer research into novel patient-specific treatment regimens in the clinic.
Journal ArticleDOI
Human organoids: model systems for human biology and medicine.
TL;DR: The applications, advantages and disadvantages of human organoids as models of development and disease and the challenges that have to be overcome for organoids to be able to substantially reduce the need for animal experiments are discussed.
Journal ArticleDOI
Pre-eclampsia: pathophysiology and clinical implications
TL;DR: Recent research has focused on placental-uterine interactions in early pregnancy, and the aim now is to translate these findings into new ways to predict, prevent, and treat pre-eclampsia.
Journal ArticleDOI
Trophoblast organoids as a model for maternal–fetal interactions during human placentation
Margherita Y. Turco,Lucy Gardner,Richard G. Kay,Russell S. Hamilton,Malwina Prater,Michael Hollinshead,A.J. McWhinnie,Laura Esposito,Ridma C. Fernando,Helen E. Skelton,Frank Reimann,Fiona M. Gribble,Andrew M. Sharkey,Steven G.E. Marsh,Stephen O'Rahilly,Myriam Hemberger,Myriam Hemberger,Graham J. Burton,Ashley Moffett +18 more
TL;DR: The generation of long-term, genetically stable organoid cultures of trophoblast that can differentiate into both syncytiotrophoblast and extravillous trophOBlast is described, which will be transformative for studying human placental development and for investigating trophoplast interactions with the local and systemic maternal environment.
Journal ArticleDOI
Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies
Collin Edington,Wen Li Kelly Chen,Emily Geishecker,Timothy Kassis,Luis R. Soenksen,Brij Bhushan,Duncan Freake,Jared Kirschner,Christian Maass,Nikolaos Tsamandouras,Jorge Valdez,Christi D. Cook,Thomas Gerard Parent,Stephen Snyder,Jiajie Yu,Emily Suter,Michael Shockley,Jason Velazquez,Jeremy J. Velazquez,Linda Stockdale,Julia P. Papps,Iris Lee,Nicholas Vann,Mario E. Gamboa,Matthew E. LaBarge,Zhe Zhong,Xin Wang,Laurie A. Boyer,Douglas A. Lauffenburger,Catherine Communal,Steven R. Tannenbaum,Cynthia L. Stokes,David Hughes,Gaurav Rohatgi,David L. Trumper,Murat Cirit,Linda G. Griffith +36 more
TL;DR: An approach to co-culture multiple different MPSs linked together physiologically on re-useable, open-system microfluidic platforms that are compatible with the quantitative study of a range of compounds, including lipophilic drugs is reported.
References
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Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.
Toshiro Sato,Daniel E. Stange,Marc Ferrante,Marc Ferrante,Robert G.J. Vries,Johan H. van Es,Stieneke van den Brink,Winan J. van Houdt,Apollo Pronk,Joost van Gorp,Peter D. Siersema,Hans Clevers +11 more
TL;DR: A technology that can be used to study infected, inflammatory, or neoplastic tissues from the human gastrointestinal tract is developed that might have applications in regenerative biology through ex vivo expansion of the intestinal epithelia.
Journal ArticleDOI
Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients
Marc van de Wetering,Hayley E. Francies,Joshua M. Francis,Joshua M. Francis,Gergana Bounova,Francesco Iorio,Apollo Pronk,Winan J. van Houdt,Joost van Gorp,Amaro Taylor-Weiner,Lennart Kester,Anne McLaren-Douglas,Joyce Blokker,Sridevi Jaksani,Sina Bartfeld,Richard Volckman,Peter van Sluis,Vivian S. W. Li,Sara Seepo,Chandra Sekhar Pedamallu,Chandra Sekhar Pedamallu,Kristian Cibulskis,Scott L. Carter,Scott L. Carter,Aaron McKenna,Michael S. Lawrence,Lee Lichtenstein,Chip Stewart,Jan Koster,Rogier Versteeg,Alexander van Oudenaarden,Julio Saez-Rodriguez,Robert G.J. Vries,Gad Getz,Gad Getz,Lodewyk F. A. Wessels,Michael R. Stratton,Ultan McDermott,Matthew Meyerson,Matthew Meyerson,Mathew J. Garnett,Hans Clevers +41 more
TL;DR: Tumor organoids are amenable to high-throughput drug screens allowing detection of gene-drug associations and may fill the gap between cancer genetics and patient trials, complement cell-line- and xenograft-based drug studies, and allow personalized therapy design.
Journal ArticleDOI
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Meritxell Huch,Craig Dorrell,Sylvia F. Boj,Johan H. van Es,Vivian S. W. Li,Marc van de Wetering,Toshiro Sato,Toshiro Sato,Karien Hamer,Nobuo Sasaki,Milton J. Finegold,Annelise Haft,Robert G.J. Vries,Markus Grompe,Hans Clevers +14 more
TL;DR: Findings indicate that previous observations concerning Lgr5+ stem cells in actively self-renewing tissues can also be extended to damage-induced stem Cells in a tissue with a low rate of spontaneous proliferation.
Journal ArticleDOI
Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver
Meritxell Huch,Helmuth Gehart,Ruben van Boxtel,Karien Hamer,Francis Blokzijl,Monique M A Verstegen,Ewa Ellis,Martien van Wenum,Sabine A. Fuchs,Joep de Ligt,Marc van de Wetering,Nobuo Sasaki,Susanne J. Boers,Hans Kemperman,Jeroen de Jonge,Jan N. M. IJzermans,Edward E. S. Nieuwenhuis,Ruurdtje Hoekstra,Stephen C. Strom,Robert R G Vries,Luc J. W. van der Laan,Edwin Cuppen,Hans Clevers +22 more
TL;DR: Conditions allowing long-term expansion of adult bile duct-derived bipotent progenitor cells from human liver opens up experimental avenues for disease modeling, toxicology studies, regenerative medicine, and gene therapy.
Journal ArticleDOI
Designer matrices for intestinal stem cell and organoid culture
Nikolce Gjorevski,Norman Sachs,Andrea Manfrin,Sonja Giger,Maiia E. Bragina,Paloma Ordóñez-Morán,Hans Clevers,Matthias P. Lutolf +7 more
TL;DR: Modular synthetic hydrogel networks are used to define the key extracellular matrix parameters that govern intestinal stem cell (ISC) expansion and organoid formation, and show that separate stages of the process require different mechanical environments and ECM components.
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Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients
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