Longitudinal SARS-CoV-2 antibody study using the Easy Check COVID-19 IgM/IgG™ lateral flow assay.
Reneé Higgins,Stephen A. Rawlings,Jamie Case,Florence Y. Lee,Clarence W Chan,Bethany Barrick,Zoe C Burger,Kiang-Teck J. Yeo,Dena Marrinucci +8 more
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In this paper, the authors found sustained levels of anti-SARS-CoV-2 antibodies past 130 days post-symptom onset (PSO), with 99% positivity observed at 31-60 days PSO.Abstract:
Since the initial identification of the novel coronavirus SARS-CoV-2 in December of 2019, researchers have raced to understand its pathogenesis and begun devising vaccine and treatment strategies. An accurate understanding of the body's temporal immune response against SARS-CoV-2 is paramount to successful vaccine development and disease progression monitoring. To provide insight into the antibody response against SARS-CoV-2, plasma samples from 181 PCR-confirmed COVID-19 patients collected at various timepoints post-symptom onset (PSO) were tested for the presence of anti-SARS-CoV-2 IgM and IgG antibodies via lateral flow. Additionally, 21 donors were tracked over time to elucidate patient-specific immune responses. We found sustained levels of anti-SARS-CoV-2 antibodies past 130 days PSO, with 99% positivity observed at 31-60 days PSO. By 61-90 days PSO, the percentage of IgM-/IgG+ results were nearly equal to that of IgM+/IgG+ results, demonstrating a shift in the immune response with a decrease in IgM antibody levels. Results from this study not only provide evidence that the antibody response to COVID-19 can persist for over 4 months, but also demonstrates the ability of Easy Check™ to monitor seroconversion and antibody response of patients. Easy Check was sufficiently sensitive to detect antibodies in patient samples as early as 1-4 days PSO with 86% positivity observed at 5-7 days PSO. Further studies are required to determine the longevity and efficacy of anti-SARS-CoV-2 antibodies, and whether they are protective against re-infection.read more
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Lateral flow assays (LFA) as an alternative medical diagnosis method for detection of virus species: The intertwine of nanotechnology with sensing strategies.
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Recent Progress on Rapid Lateral Flow Assay-Based Early Diagnosis of COVID-19
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SARS-CoV-2 Antibody Prevalence and Population-Based Death Rates, Greater Omdurman, Sudan
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Titers, Prevalence, and Duration of SARS-CoV-2 Antibodies in a Local COVID-19 Outbreak and Following Vaccination
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TL;DR: It was found that antibody responses induced by vaccination were significantly higher than those induced by natural infection, suggesting that vaccination is still critical even for those naturally infected or diagnosed with COVID-19.
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Retrospective mortality and prevalence of SARS-CoV-2 antibodies in greater Omdurman, Sudan: a population-based cross-sectional survey
Wendelin Moser,Mohammed Ahmed Hassan Fahal,Elamin Abualas,Shahinaz Bedri,Mahgoub Taj Elsir,Mona Fateh El Rahman Omer Mohamed,Abdelhalim Babiker Mahmoud,Abdelhalim Babiker Mahmoud,Amna Ismail Ibrahim Ahmad,Mohammed A. Mohammed,Mohammed A. Mohammed,Sami Altalib,Sami Altalib,Ola Adil DafaAllah,Salahaldin Abdallah Hmed,Andrew S. Azman,Andrew S. Azman,Andrew S. Azman,Iza Ciglenecki,Etienne Gignoux,Alan Gonzalez,Christine Mwongera,Manuel Albela +22 more
TL;DR: In this paper, the authors conducted a survey to estimate the COVID-19 related mortality and cumulative incidence of SARS-CoV-2 infections in Omdurman, Sudan.
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TL;DR: It is suggested that SARS-CoV2-specific IgG or IgM seroconversion occurs within 20 days post symptom onset and may be helpful for the diagnosis of suspected patients with negative RT–PCR results and for the identification of asymptomatic infections.
Journal ArticleDOI
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Quanxin Long,Xiaojun Tang,Qiu Lin Shi,Qin Li,Hai Jun Deng,Jun Yuan,Jie Li Hu,Wei Xu,Yong Zhang,Fa Jin Lv,Kun Su,Fan Zhang,Jiang Gong,Bo Wu,Xia Mao Liu,Jin Jing Li,Jing Fu Qiu,Juan Chen,Ailong Huang +18 more
TL;DR: A cohort of asymptomatic patients infected with SARS-CoV-2 had significantly lower levels of virus-specific IgG antibodies compared to a cohort of age- and sex-matched symptomatic infected patients.
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