Loss of Nfkb1 leads to early onset aging
Giovanna M. Bernal,Joshua S. Wahlstrom,Clayton D. Crawley,Kirk E. Cahill,Peter Pytel,Hua Liang,Shijun Kang,Ralph R. Weichselbaum,Bakhtiar Yamini +8 more
- Vol. 6, Iss: 11, pp 931-943
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TLDR
Data show that loss of Nfkb1 leads to early animal aging that is associated with reduced apoptosis and increased cellular senescence, and support the strong link between the NF-(B pathway and mammalian aging.Abstract:
NF-κB is a major regulator of age-dependent gene expression and the p50/NF-κB1 subunit is an integral modulator of NF-κB signaling. Here, we examined Nfkb1-/- mice to investigate the relationship between this subunit and aging. Although Nfkb1-/- mice appear similar to littermates at six months of age, by 12 months they have a higher incidence of several observable age-related phenotypes. In addition, aged Nfkb1-/- animals have increased kyphosis, decreased cortical bone, increased brain GFAP staining and a decrease in overall lifespan compared to Nfkb1+/+. In vitro, serially passaged primary Nfkb1-/- MEFs have more senescent cells than comparable Nfkb1+/+ MEFs. Also, Nfkb1-/- MEFs have greater amounts of phospho-H2AX foci and lower levels of spontaneous apoptosis than Nfkb1+/+, findings that are mirrored in the brains of Nfkb1-/- animals compared to Nfkb1+/+. Finally, in wildtype animals a substantial decrease in p50 DNA binding is seen in aged tissue compared to young. Together, these data show that loss of Nfkb1 leads to early animal aging that is associated with reduced apoptosis and increased cellular senescence. Moreover, loss of p50 DNA binding is a prominent feature of aged mice relative to young. These findings support the strong link between the NF-κB pathway and mammalian aging.read more
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ATM is a key driver of NF-κB-dependent DNA-damage-induced senescence, stem cell dysfunction and aging.
Jing Zhao,Jing Zhao,Jing Zhao,Lei Zhang,Lei Zhang,Aiping Lu,Yingchao Han,Debora Colangelo,Debora Colangelo,Christina Bukata,Alex C. Scibetta,Matthew J. Yousefzadeh,Matthew J. Yousefzadeh,Xuesen Li,Aditi U. Gurkar,Sara J. McGowan,Sara J. McGowan,Luise Angelini,Luise Angelini,Ryan D. O’Kelly,Ryan D. O’Kelly,Hongshuai Li,Lana Corbo,Tokio Sano,Heather Nick,Enrico Pola,Smitha P.S. Pilla,Warren C. Ladiges,Nam Vo,Johnny Huard,Laura J. Niedernhofer,Laura J. Niedernhofer,Paul D. Robbins,Paul D. Robbins +33 more
TL;DR: It is demonstrated that the ATM kinase is a major mediator of DNA damage-induced, NF-κB-mediated cellular senescence, stem cell dysfunction and aging and thus represents a therapeutic target to slow the progression of aging.
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Differentially expressed genes and gene networks involved in pig ovarian follicular atresia
Elena Terenina,Stéphane Fabre,Agnès Bonnet,Danielle Monniaux,Christèle Robert-Granié,Magali SanCristobal,Julien Sarry,Florence Vignoles,Florence Gondret,Florence Gondret,Philippe Monget,Gwenola Tosser-Klopp +11 more
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"Cell Membrane Theory of Senescence" and the Role of Bioactive Lipids in Aging, and Aging Associated Diseases and Their Therapeutic Implications.
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