Low intermittent flow promotes rat mesenchymal stem cell differentiation in logarithmic fluid shear device
Reads0
Chats0
TLDR
The results suggest that the intermittent shear of the order of 10 mPa can competently enhance osteogenic differentiation of rBMSCs compared to static culture.Abstract:
Bone marrow mesenchymal stem cells are an ideal candidate for bone tissue engineering due to their osteogenic potential. Along with chemical, mechanical signals such as fluid shear stress have been found to influence their differentiation characteristics. But the range of fluid shear experienced in vivo is too wide and difficult to generate in a single device. We have designed a microfluidic device that could generate four orders of shear stresses on adherent cells. This was achieved using a unique hydraulic resistance combination and linear optimization to the lesser total length of the circuit, making the device compact and yet generating four logarithmically increasing shear stresses. Numerical simulation depicts that, at an inlet velocity of 160 μl/min, our device generated shear stresses from 1.03 Pa to 1.09 mPa. In this condition, we successfully cultured primary rat bone marrow mesenchymal stem cells (rBMSCs) in the device for a prolonged period of time in the incubator environment (four days). Higher cell proliferation rate was observed in the intermittent flow at 1.09 mPa. At 10 mPa, both upregulation of osteogenic genes and higher alkaline phosphatase activity were observed. These results suggest that the intermittent shear of the order of 10 mPa can competently enhance osteogenic differentiation of rBMSCs compared to static culture.read more
Citations
More filters
Posted ContentDOI
Measuring the density and viscosity of culture media for optimized computational fluid dynamics analysis of in vitro devices
TL;DR: The density and dynamic viscosity of DMEM and RPMI-1640 media supplemented with typical concentrations of foetal bovine serum were measured to serve as a reference for computational design analysis and demonstrated clear differences in maximum wall shear stress and pressure between fluid models.
Journal ArticleDOI
Mechanical Stimulation on Mesenchymal Stem Cells and Surrounding Microenvironments in Bone Regeneration: Regulations and Applications
Yuyang Sun,Ben Wan,Renxian Wang,Bo-Wen Zhang,P Luo,Diaodiao Wang,Jing‐Jun Nie,Dafu Chen,Xinbao Wu +8 more
TL;DR: The mechanotransduction mechanisms of MSCs, the regulation of mechanical stimulation on microenvironments surrounding MSCS by modulating the immune response, angiogenesis and osteogenesis, and the application of mechanical stimulated bone regeneration are reviewed.
Journal ArticleDOI
Induction of osteogenic differentiation of bone marrow stromal cells on 3D polyester-based scaffolds solely by subphysiological fluidic stimulation in a laminar flow bioreactor:
TL;DR: The fatal determination of bone marrow mesenchymal stem/stromal cells (BMSC) is closely associated with mechano-environmental factors in addition to biochemical clues as mentioned in this paper.
Journal ArticleDOI
Biophysical Stimuli as the Fourth Pillar of Bone Tissue Engineering.
Zhuowen Hao,Zhenhua Xu,Xuan Wang,Yi Wang,Hanke Li,Tianhong Chen,Yingkun Hu,Renxin Chen,Kegang Huang,Chao Chen,Jingfeng Li +10 more
TL;DR: In this paper, the osteoinductive mechanisms of biophysical transduction (a combination of mechanotransduction and electrocoupling) are reviewed to direct the osteogenic differentiation of mesenchymal stem cells (MSCs).
Journal ArticleDOI
How the mechanical microenvironment of stem cell growth affects their differentiation: a review
TL;DR: In this article , the effects of mechanical factors in the stem cell growth microenvironment on stem cell differentiation, how mechanical signals are transmitted to and function within the cell, and the influence on the use of stem cells in clinical applications.
References
More filters
Journal ArticleDOI
Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease.
Valentina Cappello,Laura Marchetti,Paola Parlanti,Paola Parlanti,Silvia Landi,Ilaria Tonazzini,Marco Cecchini,Vincenzo Piazza,Mauro Gemmi +8 more
TL;DR: The data further characterize the ultrastructural analysis of the KD mouse model, and support recent theories of a dying-back mechanism for neuronal degeneration, which is independent of demyelination.
Journal ArticleDOI
Mechanical characterization of bulk Sylgard 184 for microfluidics and microengineering
TL;DR: In this article, the authors report an investigation of the variation in the mechanical properties of bulk polydimethylsiloxane (PDMS) elastomers with curing temperature, over the range 25 ◦ C to 200 ¼ C, over a range up to 40% strain and hardness of 44−54 ShA.
Journal ArticleDOI
The dynamic response of vascular endothelial cells to fluid shear stress.
TL;DR: Preliminary studies indicate that certain endothelial cell functions, including fluid endocytosis, cytoskeletal assembly and nonthrombogenic surface properties, also are sensitive to shear stress, which suggests that fluid mechanical forces can directly influence endothelialcell structure and function.
Journal ArticleDOI
A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow
Masoud Soleimani,Samad Nadri +1 more
TL;DR: A protocol for straightforward isolation and culture of mesenchymal stem cells (MSCs) from mouse bone marrow (BM) is explained to supply researchers with a method that can be applied in cell biology and tissue engineering with minimal requirements.
Journal ArticleDOI
Endothelial fluid shear stress sensing in vascular health and disease
TL;DR: Viewing atherosclerosis and vascular malformations as instances of pathological morphogenesis provides a unifying perspective that may aid in developing new therapies.