Open AccessBook
Lymphocytes : a practical approach
Reads0
Chats0
TLDR
The objective is to establish a protocol for quantification of antigen-specific T-cells HLA -peptide tetrameric complexes and investigate the role of T-cell reprograming in the selection of lymphocytes for HLA typing.Abstract:
Preface Preparation of lymphocytes and idenfication of lymphocyte subpopulations Immunohistochemistry of lymphoid organs T and B-cell hybridomas Murine T-cell culture Human CD4+ T-cell culture Human Cytotoxic T-cell culture Limiting dilution analysis for quantification of antigen-specific T-cells HLA -peptide tetrameric complexes Expansion of human T-cells for immunotheraphy HLA typing Characterisation of lymphocyte surface markers Apoptosis assays for lymphocytes Thymic organ culture Indexread more
Citations
More filters
Journal ArticleDOI
The relationship of 5HTT (SLC6A4) methylation and genotype on mRNA expression and liability to major depression and alcohol dependence in subjects from the Iowa Adoption Studies
Robert A. Philibert,Harinder K. Sandhu,Nancy Hollenbeck,Tracy D. Gunter,William Adams,Anup Madan +5 more
TL;DR: It is concluded that both genetic variation and epigenetic modifications contribute to the regulation of SLC6A4 function and that more in‐depth studies of the molecular mechanisms controlling gene activity and the relationship of these mechanisms to behavioral illness are indicated.
Journal ArticleDOI
MAOA Methylation is Associated with Nicotine and Alcohol Dependence in Women
TL;DR: It is concluded that methylation of MAOA may play a significant role in common psychiatric illness and that further examination of epigenetic processes at this locus is in order.
Journal ArticleDOI
Depletion of NK cells results in disseminating lethal infection with Bordetella pertussis associated with a reduction of antigen-specific Th1 and enhancement of Th2, but not Tr1 cells
TL;DR: It is suggested that NK cells confer resistance to B. pertussis by activating IL‐12‐mediated production of IFN‐γ, which enhances the anti‐bacterial activity of macrophages, but also promotes the differentiation of Th1 cells.
Journal ArticleDOI
Matrix metalloproteinase inhibition modulates fibroblast-mediated matrix contraction and collagen production in vitro.
Julie T. Daniels,Alison D. Cambrey,N L Occleston,Qian Garrett,Roy W. Tarnuzzer,Gregory S. Schultz,Peng T. Khaw +6 more
TL;DR: Broad-spectrum MMP inhibition significantly reduced matrix contraction and production without cell toxicity and future clinical use of MMP inhibitors may be possible, because M MP inhibition significantly reduces fibroblast functions associated with contractile scarring.
Journal ArticleDOI
Adenylate cyclase toxin from Bordetella pertussis synergizes with lipopolysaccharide to promote innate interleukin-10 production and enhances the induction of Th2 and regulatory T cells.
TL;DR: It is demonstrated that activation of innate cells in vitro by CyaA is dependent on a second signal through a TLR and that Cya a can promote Th2/Tr1-cell responses by inhibiting IL-12 and promoting IL-10 production by DC and macrophages.