Journal ArticleDOI
Magnesium-induced biofilm development in Arthrobacter sp. SUK 1201 and removal of hexavalent chromium
Satarupa Dey,Amal K. Paul +1 more
Reads0
Chats0
TLDR
Both Scanning and Confocal Laser Scanning Microscopy revealed that biofilms formed under the induction of Mg(II) had characteristic higher cell densities, suggesting its possible implication in effective bioremediation of chromium polluted environments.Abstract:
This study reports the influence of Mg ions on the development and architecture of biofilms by a chromium resistant and reducing bacterium Arthrobacter sp. SUK 1201 and their utilization in the removal of toxic hexavalent chromium. Among the different metal ions tested, Mg(II) greatly influenced the biofilm growth in peptone yeast extract glucose medium. Both Scanning and Confocal Laser Scanning Microscopy revealed that biofilms formed under the induction of Mg(II) had characteristic higher cell densities. The cells remain embedded in thick porous layers of extracellular polymeric substances as evident from the fluorescein isothiocyanate labeled lectin concanavalin A and 4, 6- diamino-2-phenylindole staining. COMSTAT analysis also indicated maximum thickness and roughness coefficient of the biofilm grown in presence of Mg(II). Biofilms of Arthrobacter sp. SUK 1201 developed under such Mg (II) influenced condition showed complete removal of 0.5 mM Cr(VI) in mineral salts medium. The biofilm of this...read more
Citations
More filters
Journal ArticleDOI
Expression of an Extracellular Protein (SMU.63) Is Regulated by SprV in Streptococcus mutans.
Satya Deo Pandey,Indranil Biswas +1 more
TL;DR: A link between SprV and an ∼67-kDa protein in the culture supernatant of strain UA159 that was later confirmed as SMU.63 was established, and it was discovered that SprV downregulates the transcription and translation of SM U.63 in S. mutans.
Journal ArticleDOI
Delineating bacterial community structure of rhizosphere metagenome from Kansal Forest
TL;DR: The present investigation was carried out to delineate the microbial diversity of rhizosphere soil using metagenomic approach, and Bacillus, Pseudomonas and Staphylococcus demonstrated abundant presence in this soil.
Turkish Journal of Biology Cr(VI) reduction by Cellulosimicrobium sp. isolated from tannery effluent
TL;DR: In this article, an indigenous chromium-resistant bacterial strain (A 8 ) was identified as Cellulosimicrobium sp. on the basis of morphological and biochemical characterization and 16S rRNA gene sequencing.
Journal ArticleDOI
Differential effects of magnesium, calcium, and sodium on Listeria monocytogenes biofilm formation
TL;DR: The study showed that the divalent cations magnesium and calcium increased biofilm formation compared with the monovalent cation, sodium, which did not have a significant effect on the initial stages ofBiofilm formation but appeared to influence the later stages of biofilm development.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
TL;DR: The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Journal ArticleDOI
The biofilm matrix
TL;DR: The functions, properties and constituents of the EPS matrix that make biofilms the most successful forms of life on earth are described.
Journal ArticleDOI
Quantification of biofilm structures by the novel computer program COMSTAT.
Arne Heydorn,Alex Toftgaard Nielsen,Morten Hentzer,Claus Sternberg,Michael Givskov,Bjarne Kjær Ersbøll,Søren Molin +6 more
TL;DR: Analysis of biofilms of P. aureofaciens growing on 0.03 mM, 0.1 mM or 0.5 mM citrate minimal media showed that mean biofilm thickness increased with increasing citrate concentration, whereas surface to volume ratio increased with higher citrate concentrations.
Related Papers (5)
Biofilm Formation of the Facultative Thermophile Bacillus pumilus D194A and Affects of Sanitation Agents on Its Biofilms
Tugba Kilic,A. Coleri Cihan +1 more