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Book ChapterDOI

Marine Bacteria—A Treasure House of Valuable Products and Functions

01 Jan 2020-pp 415-436
TL;DR: In this paper, the authors discuss about the production of various bioactive compounds, enzymes, neutraceuticals, exopolysaccharides, antibiotics, biosurfactants as well as the potential organisms useful in dye decolorization, microbial enhanced oil recovery, hydrocarbon degradation, and metal bioremediation.
Abstract: Immense diversity of prokaryotes is reported for the oceans; however, we probably know only 0.01% of the microorganisms from marine ecosystems. Due to the burning need of new or novel therapeutic and environmental useful compounds, these less explored marine habitats have attracted various researchers since the last five decades. Nearly 16,000 natural products are discovered from marine organisms. Marine microbes are very diverse because they are exposed to wide variations in temperature, salinity, nutrition, and pressure at different levels. These extreme conditions are responsible for the presence of diverse photoautotrophs, chemolithotrophs, heterotrophs, nitrogen fixers, denitrifiers, luminescent as well as sulfur and iron oxidizers and reducing microorganisms. Various culture-dependent and -independent methods are used to explore the hidden microbial diversity and their potency. The chapter discusses about the production of various bioactive compounds, enzymes, neutraceuticals, exopolysaccharides, antibiotics, biosurfactants as well as the potential organisms useful in dye decolorization, microbial enhanced oil recovery, hydrocarbon degradation, and metal bioremediation. The chapter also deals with feature prospects in terms of valuable industrial and environmental significance of the bacterial community of oceans.
Citations
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Journal ArticleDOI
TL;DR: Marine pollution has been increasing over the years and can impact directly living organisms as discussed by the authors , and the continued pollution of soil and fresh water by agriculture, industrial and urban activities frequently reaches the rivers and the ocean by run over polluting from these environments.
Abstract: Marine pollution has been increasing over the years and can impact directly living organisms. The continued pollution of soil and fresh water by agriculture, industrial and urban activities frequently reaches the rivers and the ocean by run over polluting from these environments. Bioremediation is an eco-friendly technique that can immobilize, reduce damage, inactivate or remove contaminants using living organisms or their structures or products for cleaning up the environment. The technique used for bioremediation depends on the type of contaminant, including structure, oxidation stage, complexation form. Bacteria are recognized as important agents in bioremediation processes, including removal of heavy metals, biodegradation of polyaromatic and halogenated hydrocarbons, petroleum and diesel, and biodegradation of plastics. Marine bacteria present a great diversity of metabolic activities and their potential for bioremediation is still poorly exploited.
References
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Journal ArticleDOI
TL;DR: Phylogenetic analysis of the retrieved rRNA sequence of an uncultured microorganism reveals its closest culturable relatives and may, together with information on the physicochemical conditions of its natural habitat, facilitate more directed cultivation attempts.

9,017 citations

Journal ArticleDOI
TL;DR: This review focuses on the architectures of bacterial chemical communication networks; how chemical information is integrated, processed, and transduced to control gene expression; how intra- and interspecies cell-cell communication is accomplished; and the intriguing possibility of prokaryote-eukaryote cross-communication.
Abstract: Bacteria communicate with one another using chemical signal molecules. As in higher organisms, the information supplied by these molecules is critical for synchronizing the activities of large groups of cells. In bacteria, chemical communication involves producing, releasing, detecting, and responding to small hormone-like molecules termed autoinducers. This process, termed quorum sensing, allows bacteria to monitor the environment for other bacteria and to alter behavior on a population-wide scale in response to changes in the number and/or species present in a community. Most quorum-sensing-controlled processes are unproductive when undertaken by an individual bacterium acting alone but become beneficial when carried out simultaneously by a large number of cells. Thus, quorum sensing confuses the distinction between prokaryotes and eukaryotes because it enables bacteria to act as multicellular organisms. This review focuses on the architectures of bacterial chemical communication networks; how c...

3,360 citations

Journal ArticleDOI
TL;DR: Rates of biodegradation depend greatly on the composition, state, and concentration of the oil or hydrocarbons, with dispersion and emulsification enhancing rates in aquatic systems and absorption by soil particulates being the key feature of terrestrial ecosystems.

2,450 citations

Journal ArticleDOI
TL;DR: Reassembly of multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and microheterogeneity, and lateral gene transfer among members of an uncultured community.
Abstract: Metagenomics (also referred to as environmental and community genomics) is the genomic analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of microorganisms. The development of metagenomics stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. This evidence was derived from analyses of 16S rRNA gene sequences amplified directly from the environment, an approach that avoided the bias imposed by culturing and led to the discovery of vast new lineages of microbial life. Although the portrait of the microbial world was revolutionized by analysis of 16S rRNA genes, such studies yielded only a phylogenetic description of community membership, providing little insight into the genetics, physiology, and biochemistry of the members. Metagenomics provides a second tier of technical innovation that facilitates study of the physiology and ecology of environmental microorganisms. Novel genes and gene products discovered through metagenomics include the first bacteriorhodopsin of bacterial origin; novel small molecules with antimicrobial activity; and new members of families of known proteins, such as an Na+(Li+)/H+ antiporter, RecA, DNA polymerase, and antibiotic resistance determinants. Reassembly of multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and microheterogeneity, and lateral gene transfer among members of an uncultured community. The application of metagenomic sequence information will facilitate the design of better culturing strategies to link genomic analysis with pure culture studies.

2,224 citations

Journal ArticleDOI
TL;DR: Three rRNA gene standards were prepared by PCR, mixed in known proportions, and amplified a second time by using primer pairs in which one primer was labeled with a fluorescent nucleotide derivative to fit a kinetic model in which the reannealing of genes progressively inhibits the formation of template-primer hybrids.
Abstract: The PCR is used widely for the study of rRNA genes amplified from mixed microbial populations. These studies resemble quantitative applications of PCR in that the templates are mixtures of homologs and the relative abundance of amplicons is thought to provide some measure of the gene ratios in the starting mixture. Although such studies have established the presence of novel rRNA genes in many natural ecosystems, inferences about gene abundance have been limited by uncertainties about the relative efficiency of gene amplification in the PCR. To address this question, three rRNA gene standards were prepared by PCR, mixed in known proportions, and amplified a second time by using primer pairs in which one primer was labeled with a fluorescent nucleotide derivative. The PCR products were digested with restriction endonucleases, and the frequencies of genes in the products were determined by electrophoresis on an Applied Biosystems 373A automated DNA sequencer in Genescan mode. Mixtures of two templates amplified with the 519F-1406R primer pair yielded products in the predicted proportions. A second primer pair (27F-338R) resulted in strong bias towards 1:1 mixtures of genes in final products, regardless of the initial proportions of the templates. This bias was strongly dependent on the number of cycles of replication. The results fit a kinetic model in which the reannealing of genes progressively inhibits the formation of template-primer hybrids.

1,898 citations