Matrix elasticity directs stem cell lineage specification.
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"Matrix elasticity directs stem cell..." refers methods or result in this paper
...ML7 will block activation of these as well as smooth muscle myosin isoforms but will not affect activation of any other myosins in MSCs. Results with ML7 prove below to be identical to those seen with blebbistatin, and so NMM II activity appears to be necessary for matrix elasticity-driven lineage specification....
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...As described in the introduction and supported by the blebbistatin and ML7 results above, the likely generators of force are the nonmuscle myosin II isoforms....
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...Both blebbistatin and ML7 suppress expression of key lineage markers (Figures 1C and 5B), consistent with NMM II activity, ultimately regulating lineage marker profiles in addition to its own expression....
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...To reinforce this conclusion and to rule out a role for myosin VI in matrix sensing, we repeated the above experiments with the myosin light chain kinase (MLCK) inhibitor, ML7 (Dhawan and Helfman, 2004)....
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...Western blots confirm similar isoform sensitivity at the protein level to blebbistatin and also to ML7, an inhibitor that also inhibits the NMM II’s through its inhibition of MLCK. NMM IIA expression only slightly downregulates, while NMM IIB expression drops about 10-fold to levels comparable to MSCs on soft gels (Figures 5B and 5C), and NMM IIC is no longer detectable....
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61 citations
"Matrix elasticity directs stem cell..." refers background in this paper
...Adult stem cells, as part of normal regenerative processes, are believed to egress and circulate away from their niche (Katayama et al., 2006), and then engraft and differentiate within a range of tissue microenvironments....
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...Possible Implications for Stem Cell Therapies Regenerative applications of stem cells are being investigated for a number of tissues, including clinical trials for postmyocardial infarction patients (Lee et al., 2004)....
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"Matrix elasticity directs stem cell..." refers background in this paper
...…Sweeney,1 and Dennis E. Discher1,2,3,4,* 1Pennsylvania Muscle Institute 2School of Engineering and Applied Science 3Cell & Molecular Biology Graduate Group 4Physics Graduate Group University of Pennsylvania, Philadelphia, PA 19104, USA *Contact: discher@seas.upenn.edu DOI 10.1016/j.cell.2006.06.044...
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...…precursor to bone is secreted by osteoblasts and reportedly is the matrix upon which MSCs undergo a transition to preosteoblasts (Figure 2D) (Andrades et al., 2001; Morinobu et al., 2003; Raisz, 1999), before the matrix calcificies over week(s) (Rattner et al., 2000) to rigid bone ( 106 kPa)....
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