Matrix elasticity directs stem cell lineage specification.
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"Matrix elasticity directs stem cell..." refers background in this paper
...Chronic inhibition of NMM II’s ATPase activity with blebbistatin (Limouze et al., 2004; Straight et al., 2003) not only inhibits morphological changes of MSCs on various matrices (Figures 1Bi and 1Bii) but also reduces transcripts levels for NMM IIA (to 50%), IIB (to 8%), and IIC (to 3...
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...…Sweeney,1 and Dennis E. Discher1,2,3,4,* 1Pennsylvania Muscle Institute 2School of Engineering and Applied Science 3Cell & Molecular Biology Graduate Group 4Physics Graduate Group University of Pennsylvania, Philadelphia, PA 19104, USA *Contact: discher@seas.upenn.edu DOI 10.1016/j.cell.2006.06.044...
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...All of the NMM II isoforms are inhibited by blebbistatin, which does not inhibit any other myosin found in MSCs (see below), other than myosin VI (Limouze et al., 2004)....
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...…in vertebrate cells without affecting MLCK. Crystal structures show inhibition of actin-activated ATPase activity by blebbistatin (Allingham et al., 2005) requires a specific alanine (or serine) residue that is found only in class II and VI myosins (Limouze et al., 2004; Straight et al., 2003)....
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"Matrix elasticity directs stem cell..." refers background or methods or result in this paper
...AFM for Matrix Elasticity and Cell Mechanics Methods Substrate elasticity was characterized per Engler et al. (2004b)....
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...Substrate elasticity was characterized per Engler et al. (2004b) ....
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...Cell Culture Human Mesenchymal Stem Cells (MSCs; Osiris Therapeutics; Baltimore, MD), human osteoblasts (hFOBs, ATCC), primary human skin fibroblasts (1F7) (Engler et al., 2004a), and murine myoblasts (C2C12s, ATCC) were cultured in normal growth media listed in Table S2....
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...Forces generated and/or imposed on the cell’s actin cytoskeleton have been postulated to influence differentiation (Engler et al., 2004a; Hinz et al., 2001; McBeath et al., 2004), but no past reports have hinted at strong, tissuedirected feedback of microenvironment elasticity on myosin expression…...
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...and coworkers was used ( Engler et al., 2004b )....
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"Matrix elasticity directs stem cell..." refers result in this paper
...…August 25, 2006 ª2006 Elsevier Inc. 685 generalizes the matrix-driven assembly of the cytoskeleton to MSCs. NMM II is already known to promote the assembly of focal adhesions (Conti et al., 2004), and so the prominent adhesions on stiffer substrates is consistent with greater activity of NMM II....
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...NMM II is already known to promote the assembly of focal adhesions (Conti et al., 2004), and so the prominent adhesions on stiffer substrates is consistent with greater activity of NMM II....
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