Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations.
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...The use of commercially fluorescent ROS probes is widespread but oftenwithout the knowledge that these do not always readout specific ROS species faithfully and are prone to other confounding artifacts (Kalyanaraman et al., 2012)....
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..., atherosclerosis, cancer, diabetes, neurodegeneration) [1]....
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"Measuring reactive oxygen and nitro..." refers background in this paper
...At a molecular level, reactive oxygen and nitrogen species exhibit signaling and cellfunction-modifying roles [2]....
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2,465 citations
"Measuring reactive oxygen and nitro..." refers background in this paper
...Previous studies implicated a role for redox-active iron in intracellular oxidation of DCFH to DCF [16], although the origin of the cellular iron was not known....
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"Measuring reactive oxygen and nitro..." refers background in this paper
...As pointed out in a recent review [3], reactive oxygen and nitrogen species are not single entities but represent a broad range of chemically distinct reactive species with diverse biological reactivities [3,4]....
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...It is unlikely to compete with H2O2-detoxifying enzymes such as the peroxiredoxins, which react 7 orders of magnitude faster with H2O2[3,4]....
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1,790 citations