Journal ArticleDOI
Mechanisms of magnesium-stimulated adhesion of osteoblastic cells to commonly used orthopaedic implants.
Hala Zreiqat,C. R. Howlett,Andrew C.W. Zannettino,Peter J. Evans,G. Schulze-Tanzil,C. Knabe,Mehdi Shakibaei +6 more
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TLDR
It is concluded that Mg(2+) supplementation of bioceramic substrata may be a promising way to improve integration of implants in orthopaedic and dental surgery.Abstract:
Poor cell adhesion to orthopaedic and dental implants may result in implant failure. Cellular adhesion to biomaterial surfaces primarily is mediated by integrins, which act as signal transduction and adhesion proteins. Because integrin function depends on divalent cations, we investigated the effect of magnesium ions modified bioceramic substrata (Al(2)O(3)-Mg(2+)) on human bone-derived cell (HBDC) adhesion, integrin expression, and activation of intracellular signalling molecules. Immunohistochemistry, flow cytometry, cell adhesion, cell adhesion blocking, and Western blotting assays were used. Our findings demonstrated that adhesion of HBDC to Al(2)O(3)-Mg(2+) was increased compared to on the Mg(2+)-free Al(2)O(3). Furthermore, HBDC adhesion decreased significantly when the fibronectin receptor alpha5beta1- and beta1-integrins were blocked by functional blocking antibodies. HBDC grown on the Mg(2+)-modified bioceramic expressed significantly enhanced levels of beta1-, alpha5beta1-, and alpha3beta1-integrins receptors compared to those grown on the native unmodified Al(2)O(3). Tyrosine phosphorylation of intracellular integrin-dependent signalling proteins as well as the expression of key signalling protein Shc isoforms (p46, p52, p66), focal adhesion kinase, and extracellular matrix protein collagen type I were significantly enhanced when HBDC were grown on Al(2)O(3)-Mg(2+) compared to the native Al(2)O(3). We conclude that cell adhesion to biomaterial surfaces is probably mediated by alpha5beta1- and beta1-integrin. Cation-promoted cell adhesion depends on 5beta1- and beta1-integrins associated signal transduction pathways involving the key signalling protein Shc and results also in enhanced gene expression of extracellular matrix proteins. Therefore, Mg(2+) supplementation of bioceramic substrata may be a promising way to improve integration of implants in orthopaedic and dental surgery.read more
Citations
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Magnesium and its alloys as orthopedic biomaterials: a review.
TL;DR: A review of the properties, biological performance, challenges and future directions of magnesium-based biomaterials can be found in this paper, where the authors explore the properties and challenges of magnesium biomaterial.
Journal ArticleDOI
A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics
TL;DR: This review comprehensively covers literature reports which have investigated specifically the effect of dissolution products of silicate bioactive glasses and glass-ceramics in relation to osteogenesis and angiogenesis and focuses on the ion release kinetics of the materials and the specific effect of the released ionic dissolution products on human cell behaviour.
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The development of binary Mg–Ca alloys for use as biodegradable materials within bone
TL;DR: The results of tensile tests and in vitro corrosion tests indicated that Mg-1Ca alloy had the acceptable biocompatibility as a new kind of biodegradable implant material and a solid alloy/liquid solution interface model was proposed to interpret the biocorrosion process and the associated hydroxyapatite mineralization.
Journal ArticleDOI
Control of biodegradation of biocompatable magnesium alloys
TL;DR: By utilising its rapid corrosion reaction and controlling its degradation process through Zn and Mn alloying, purification and anodization, chemically active magnesium can be developed into a biodegradable biocompatible implant material with a specific biodegradation process and tolerable hydrogen evolution rate.
Journal ArticleDOI
Research on an Mg-Zn alloy as a degradable biomaterial.
Shaoxiang Zhang,Xiaonong Zhang,Changli Zhao,Jianan Li,Yang Song,Chaoying Xie,Hairong Tao,Yan Zhang,Yaohua He,Yao Jiang,Yujun Bian +10 more
TL;DR: The results suggested that the novel Mg-Zn binary alloy had good biocompatibility in vivo, and no adverse effects of hydrogen generated by degradation had been observed and also no negative effects caused by the release of zinc were detected.
References
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Integrins: versatility, modulation, and signaling in cell adhesion.
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Integrins and other cell adhesion molecules.
TL;DR: Recent data describing the structure and function of some of these cell adhesion molecules are summarized and the possible role of these molecules in development, inflammation, wound healing, coagulation, and tumor metastasis is discussed.
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Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice
Dusko Ilic,Yasuhide Furuta,Satoshi Kanazawa,Naoki Takeda,Kenji Sobue,Norio Nakatsuji,S Nomura,Jiro Fujimoto,Masato Okada,Tadashi Yamamoto +9 more
TL;DR: Surprisingly, the number of focal adhesions was increased in FAK-deficient cells, suggesting that FAK may be involved in the turnover of focalAdhesion contacts during cell migration.
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Integrins: Emerging Paradigms of Signal Transduction
TL;DR: Integrins receive signals from other receptors that lead to activation of ligand binding (inside-out signaling) and matrix assembly and activate intracellular signaling pathways that converse with pathways initiated by soluble ligands to regulate cell functions.
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Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase
TL;DR: It is shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes SH2-domain-mediated association of the GRB2 adaptor protein and the c-Src protein-tyrosine kinase (PTK) with FAK in vivo, and also results in activation of mitogen-activated protein Kinase (MAPK).