Journal ArticleDOI
Mechanisms Regulating PRC2 Recruitment and Enzymatic Activity
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TLDR
A rich and fast-evolving picture of the biochemical signals that govern PRC2 function is produced, with many exciting questions still remaining.About:
This article is published in Trends in Biochemical Sciences.The article was published on 2017-07-01. It has received 171 citations till now. The article focuses on the topics: PRC2 & Chromatin.read more
Citations
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Journal ArticleDOI
An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer
Marian L. Burr,Marian L. Burr,Marian L. Burr,Christina E. Sparbier,Christina E. Sparbier,Kah Lok Chan,Kah Lok Chan,Yih-Chih Chan,Ariena Kersbergen,Enid Y.N. Lam,Enid Y.N. Lam,Elizabeth Azidis-Yates,Dane Vassiliadis,Dane Vassiliadis,Charles C. Bell,Charles C. Bell,Omer Gilan,Omer Gilan,Susan Jackson,Lavinia Tan,Lavinia Tan,Stephen Q. Wong,Stephen Q. Wong,Sebastian Hollizeck,Sebastian Hollizeck,Ewa M. Michalak,Ewa M. Michalak,Hannah V. Siddle,Michael T. McCabe,Rab K. Prinjha,Glen R. Guerra,Glen R. Guerra,Benjamin Solomon,Benjamin Solomon,Shahneen Sandhu,Shahneen Sandhu,Sarah-Jane Dawson,Sarah-Jane Dawson,Paul A. Beavis,Paul A. Beavis,Richard W. Tothill,Carleen Cullinane,Carleen Cullinane,Paul J. Lehner,Kate D. Sutherland,Kate D. Sutherland,Mark A. Dawson,Mark A. Dawson +47 more
TL;DR: A genome-wide CRISPR/Cas9 screen identified an evolutionarily conserved function of polycomb repressive complex 2 (PRC2) that mediates coordinated transcriptional silencing of the MHC-I antigen processing pathway (M HC-I APP), promoting evasion of T cell-mediated immunity.
Journal ArticleDOI
Capturing the Onset of PRC2-Mediated Repressive Domain Formation.
Ozgur Oksuz,Varun Narendra,Chul Hwan Lee,Nicolas Descostes,Gary LeRoy,Ramya Raviram,Lili Blumenberg,Kelly R. Karch,Pedro P. Rocha,Pedro P. Rocha,Benjamin A. Garcia,Jane A. Skok,Danny Reinberg +12 more
TL;DR: The principles of de novo establishment of PRC2-mediated repressive domains across the genome are demonstrated and shown to be effective at both the cis and far-cis levels.
Journal ArticleDOI
PRC2 is high maintenance
Jia Ray Yu,Jia Ray Yu,Chul Hwan Lee,Chul Hwan Lee,Ozgur Oksuz,Ozgur Oksuz,James M. Stafford,James M. Stafford,Danny Reinberg,Danny Reinberg +9 more
TL;DR: In this review, Yu et al. discuss the recent advances in knowledge of the Polycomb-repressive complex 2 (PRC2) in mammalian systems and discuss important discoveries on Polycomb function derived from model organisms in the context of understanding mammalian PRC2 function.
Journal ArticleDOI
Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA.
Xueyin Wang,Richard D. Paucek,Richard D. Paucek,Anne R. Gooding,Anne R. Gooding,Zachary Z. Brown,Eva J. Ge,Tom W. Muir,Thomas R. Cech,Thomas R. Cech +9 more
TL;DR: A model for PRC2 recruitment and an explanation for how actively transcribed genomic regions bindPRC2 but escape silencing are provided.
Journal ArticleDOI
The Complexity of PRC2 Subcomplexes.
TL;DR: This work elaborate on how the facultative PRC2 subunits regulate catalytic activity, locus-specificPRC2 binding, and propagation of H3K27me3, and how this affects chromatin structure, gene expression, and cell fate.
References
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Journal ArticleDOI
Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
John L. Rinn,Michael Kertesz,Jordon K. Wang,Sharon L. Squazzo,Xiao Xu,Samantha A. Brugmann,L. Henry Goodnough,Jill A. Helms,Peggy J. Farnham,Eran Segal,Howard Y. Chang +10 more
TL;DR: The transcriptional landscape of the four human HOX loci is characterized at five base pair resolution in 11 anatomic sites and 231 HOX ncRNAs are identified that extend known transcribed regions by more than 30 kilobases, suggesting transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance.
Journal ArticleDOI
Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
Ru Cao,Liangjun Wang,Hengbin Wang,Li Xia,Hediye Erdjument-Bromage,Paul Tempst,Richard S. Jones,Yi Zhang +7 more
TL;DR: The purification and characterization of an EED-EZH2 complex, the human counterpart of the Drosophila ESC-E(Z) complex, is reported, and it is demonstrated that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3-K27).
Journal ArticleDOI
Regulation of chromatin structure by site-specific histone H3 methyltransferases
Stephen Rea,Frank Eisenhaber,Dónal O'Carroll,Brian D. Strahl,Zu-Wen Sun,Manfred Schmid,Susanne Opravil,Karl Mechtler,Chris P. Ponting,C D Allis,Thomas Jenuwein +10 more
TL;DR: A functional interdependence of site-specific H3 tail modifications is revealed and a dynamic mechanism for the regulation of higher-order chromatin is suggested.
Journal ArticleDOI
The Polycomb complex PRC2 and its mark in life
Raphaël Margueron,Danny Reinberg +1 more
TL;DR: This work has uncovered a role for non-coding RNA in the recruitment of PRC2 to target genes, and expanded the perspectives on its function and regulation.
Journal ArticleDOI
Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein
TL;DR: The isolation of a multiprotein E(z) complex that contains extra sex combs, suppressor of zeste-12, and the histone binding proteins RbAp46/RbAp48 is reported, which possesses HMT activity with specificity for Lys 9 (K9) and Lys 27 (K27) of histone H3.
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