MeCP2 binds to 5hmc enriched within active genes and accessible chromatin in the nervous system
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In this paper, a quantitative, genome-wide analysis of 5hmC, 5-methylcytosine (5mC), and gene expression in differentiated CNS cell types in vivo is presented.Abstract:
SUMMARY The high level of 5-hydroxymethylcytosine (5hmC) present in neuronal genomes suggests that mechanisms interpreting 5hmC in the CNS may differ from those present in embryonic stem cells. Here, we present quantitative, genome-wide analysis of 5hmC, 5-methylcytosine (5mC), and gene expression in differentiated CNS cell types in vivo. We report that 5hmC is enriched in active genes and that, surprisingly, strong depletion of 5mC is observed over these regions. The contribution of these epigenetic marks to gene expression depends critically on cell type. We identify methyl-CpG-binding protein 2 (MeCP2) as the major 5hmC-binding protein in the brain and demonstrate that MeCP2 binds 5hmC- and 5mC-containing DNA with similar high affinities. The Rett-syndrome-causing mutation R133C preferentially inhibits 5hmC binding. These findings support a model in which 5hmC and MeCP2 constitute a cell-specific epigenetic mechanism for regulation of chromatin structure and gene expression.read more
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The Impact of Environmental Factors on 5-Hydroxymethylcytosine in the Brain
TL;DR: Environment appears to shape 5-hmC levels in the brain, but the available literature is hampered by limitations in measurement methods, and the field of neuroepigenetics needs to adopt new tools to increase the specificity of its data and enhance biological interpretation of exposure-related changes in 5-HMC.
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Tagger-A Swiss army knife for multiomics to dissect cell type-specific mechanisms of gene expression in mice
Lech Kaczmarczyk,Lech Kaczmarczyk,Vikas Bansal,Ashish Rajput,Raza-Ur Rahman,Wiesław Krzyżak,Joachim Degen,Stefanie Poll,Martin Fuhrmann,Stefan Bonn,Stefan Bonn,Walker S. Jackson,Walker S. Jackson +12 more
TL;DR: It is demonstrated that Tagger can capture the desired nucleic acids, enabling multiple omics approaches to be applied to specific cell types in vivo using a single transgenic mouse line.
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Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue
Owen M. Wilkins,Kevin C. Johnson,E. Andres Houseman,Jessica E. King,Carmen J. Marsit,Brock C. Christensen +5 more
TL;DR: These data suggest 5hmC may participate in gene regulatory programs that are dysregulated during breast-related carcinogenesis and provide a genome-wide map of nucleotide-level 5mC in normal breast tissue.
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Epigenetic regulation of Fgf1 transcription by CRTC1 and memory enhancement
TL;DR: The role of histone acetylation and DNA methylation are discussed, which are two best understood epigenetic processes in memory processes, and learning-strength-dependent changes in chromatin on the fibroblast growth factor 1 gene and on the molecular events that modulate regulation of Fgf1 transcription are focused on.
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The Molecular Functions of MeCP2 in Rett Syndrome Pathology.
Osman Sharifi,Dag H. Yasui +1 more
TL;DR: The full scope of MeCP2 research available in the NIH Pubmed (https://pubmed.ncbi.nlm.nih.gov/) data base to date is considered in this article.
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