Medium development for xanthan production
TL;DR: Mineral sources such as CaCO3 and KH2PO4 proved to be important factors influencing polysaccharide production and quality and the use of high carbon to nitrogen ratios in the defined medium resulted in a major increase in the broth consistency index.
About: This article is published in Process Biochemistry.The article was published on 1992-05-01. It has received 83 citations till now. The article focuses on the topics: Carbon-to-nitrogen ratio.
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TL;DR: Using a genetic algorithm, it has been experimentally verified that process improvements can be achieved both for microbial and enzymatic conversions and for cell cultures despite the large number of medium components under simultaneous consideration, in exploring a new variable space.
220 citations
TL;DR: In this paper, the authors focus on the application, development, and practical considerations of fermentations in chemically defined media in an industrial environment and present a review of the application and process economics of such media.
Abstract: The use of chemically defined media is gaining popularity in some commercial fermentations, particularly for the preparation of biological products. Although these media are still not frequently developed for industrial processes, they do exhibit favorable characteristics at large scale that are not observed with traditional complex media. This review focuses on the application, development, and practical considerations, especially process economics, of fermentations in chemically defined media in an industrial environment.
204 citations
Cites methods from "Medium development for xanthan prod..."
...…those variables (three to ®ve) that have a signi®cant e ect on the desired response from a larger number of potential variables (more than ®ve) with a minimum of testing, employing fractional factorial designs such as the Plackett-Burman method (Plackett and Burman 1946; Roseiro et al. 1992)....
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TL;DR: A newly isolated strain, TIT-90076, which produces β-fructofuranosidase (FFase, EC 3.2.1.26), with a high transfructosylating activity was identified as Aspergillus japonicus, and sucrose is the best carbon source and yeast extract the best nitrogen source for enzyme production.
153 citations
TL;DR: Proper understanding and rigorous analysis of several engineering parameters would pave the way towards the successful commercialization of plant cell bioprocesses.
Abstract: Plant cell culture provides a viable alternative over whole plant cultivation for the production of secondary metabolites. In order to successfully cultivate the plant cells at large scale, several engineering parameters such as, cell aggregation, mixing, aeration, and shear sensitivity are taken into account for selection of a suitable bioreactor. The media ingredients, their concentrations and the environmental factors are optimized for maximal synthesis of a desired metabolite. Increased productivity in a bioreactor can be achieved by selection of a proper cultivation strategy (batch, fed-batch, two-stageetc.), feeding of metabolic precursors and extraction of intracellular metabolites. Proper understanding and rigorous analysis of these parameters would pave the way towards the successful commercialization of plant cell bioprocesses.
139 citations
TL;DR: In this article, the authors investigated the effects of fermentation time, temperature and broth content on mycelial growth in terms of dry cell weight (DCW) and extracellular polysaccharide content (EPC) produced by the fungus Boletus spp. in submerged fermentation.
120 citations
References
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TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
225,085 citations
3,546 citations
TL;DR: In this paper, a set of points lying on concentric spherical shells are generated which have an equally spaced distribution of points and have uniform space-filling properties and are tabulated up to ten factors.
Abstract: Designs are generated which have an equally spaced distribution of points lying on concentric spherical shells. They have uniform space‐filling properties and are tabulated up to ten factors. The designs are shown to be more uniform than familiar experimental designs on the basis of two measures of uniformity. Their use is illustrated by an example with four factors.
621 citations
TL;DR: Rate and yield information relating to biomass and product formation and to nitrogen, glucose and oxygen consumption are described for xanthan gum batch fermentations in which both chemically defined and complex media are employed.
Abstract: Rate and yield information relating to biomass and product formation and to nitrogen, glucose and oxygen consumption are described for xanthan gum batch fermentations in which both chemically defined (glutamate nitrogen) and complex (peptone nitrogen) media are employed. Simple growth and product models are used for data interpretation. For nitrogen sources, rate and yield parameter estimates are shown to be independent of initial nitrogen concentrations. For stationary phases, specific rates of gum production are shown to be independent of nitrogen source but dependent on initial nitrogen concentration. The latter is modeled empirically and suggests caution in applying simple product models to xanthan gum fermentations.
92 citations