Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid.
M J Worsey,P A Williams +1 more
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TLDR
Pseudomonas putida (arvilla) mt-2 carries genes for the catabolism of toluene, m- xylene, and p-xylene on a transmissible plasmid, TOL, suggesting that a single set of nonspecific enzymes is responsible for the dissimilation of the breakdown products of toLUene and m- and p -xylene.Abstract:
Pseudomonas putida (arvilla) mt-2 carries genes for the catabolism of toluene, m-xylene, and p-xylene on a transmissible plasmid, TOL. These compounds are degraded by oxidation of one of the methyl substituents via the corresponding alcohols and aldehydes to benzoate and m- and p-toluates, respectively, which are then further metabolised by the meta pathway, also coded for by the TOL plasmid. The specificities of the benzyl alcohol dehydrogenase and the benzaldehyde dehydrogenase for their three respective substrates are independent of the carbon source used for growth, suggesting that a single set of nonspecific enzymes is responsible for the dissimilation of the breakdown products of toluene and m- and p-xylene. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase are coincidently and possible coordinately induced by toluene and the xylenes, and by the corresponding alcohols and aldehydes. They are not induced in cells grown on m-toluate but catechol 2,3-oxygenase can be induced by m-xylene.read more
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References
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Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid.
Peter A. Williams,Keith Murray +1 more
TL;DR: Preliminary results from mutant strains of Pseudomonas putida mt-2 and from a mutant defective in the regulation of the plasmid-carried pathway suggest that the wild type contains two benzoate oxidase systems, one on thePlasmid which is nonspecific in both its catalysis and its induction and one in the chromosome which is more specific to benzoates as substrate and is specifically induced by benzosate.
Journal ArticleDOI
Transmissible Plasmid Coding Early Enzymes of Naphthalene Oxidation in Pseudomonas putida
N. W. Dunn,I. C. Gunsalus +1 more
TL;DR: The capacity of Pseudomonas putida PpG7 to grow on naphthalene, phenotype Nah(+), is lost spontaneously, and the frequency is increased by treatment with mitomycin C, and this character can be transferred to cured or heterologous fluorescent pseudomonads lacking this capacity by conjugation.
Journal ArticleDOI
The meta Cleavage of Catechol by Azotobacter Species
TL;DR: Catechol was metabolized through 2-hydroxymuconic semialdehyde by cell-free extracts of benzoate-grown Azotobacter Strains and the findings described here are discussed in connection with the two previously reported meta cleavage pathways for the oxidation of catechol in Pseudomonas strains.
Journal ArticleDOI
The Conversion of Catechol and Protocatechuate to β-Ketoadipate by Pseudomonas putida IV. REGULATION
TL;DR: Moraxella lwoffii, which degrades protocatechuate and catechol through identical step-reactions, regulates the synthesis of the enzymes mediating these conversions by a different mechanism.
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