Metal Ion Recognition in Ligands with Negatively Charged Oxygen Donor Groups. Complexation of Fe(III), Ga(III), In(III), Al(III), and Other Highly Charged Metal Ions.
About: This article is published in ChemInform.The article was published on 1989-09-19. It has received 51 citations till now. The article focuses on the topics: Metal ions in aqueous solution.
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TL;DR: Bacteria were identified by 16S rRNA sequence analysis, and isolates were further characterized regarding characteristics that may be relevant for a beneficial plant-microbe interaction—Ni tolerance, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and siderophore production.
Abstract: Thlaspi goesingense is able to hyperaccumulate extremely high concentrations of Ni when grown in ultramafic soils. Recently it has been shown that rhizosphere bacteria may increase the heavy metal concentrations in hyperaccumulator plants significantly, whereas the role of endophytes has not been investigated yet. In this study the rhizosphere and shoot-associated (endophytic) bacteria colonizing T. goesingense were characterized in detail by using both cultivation and cultivation-independent techniques. Bacteria were identified by 16S rRNA sequence analysis, and isolates were further characterized regarding characteristics that may be relevant for a beneficial plant-microbe interaction—Ni tolerance, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and siderophore production. In the rhizosphere a high percentage of bacteria belonging to the Holophaga/Acidobacterium division and α-Proteobacteria were found. In addition, high-G+C gram-positive bacteria, Verrucomicrobia, and microbes of the Cytophaga/Flexibacter/Bacteroides division colonized the rhizosphere. The community structure of shoot-associated bacteria was highly different. The majority of clones affiliated with the Proteobacteria, but also bacteria belonging to the Cytophaga/Flexibacter/Bacteroides division, the Holophaga/Acidobacterium division, and the low-G+C gram-positive bacteria, were frequently found. A high number of highly related Sphingomonas 16S rRNA gene sequences were detected, which were also obtained by the cultivation of endophytes. Rhizosphere isolates belonged mainly to the genera Methylobacterium, Rhodococcus, and Okibacterium, whereas the majority of endophytes showed high levels of similarity to Methylobacterium mesophilicum. Additionally, Sphingomonas spp. were abundant. Isolates were resistant to Ni concentrations between 5 and 12 mM; however, endophytes generally tolerated higher Ni levels than rhizosphere bacteria. Almost all bacteria were able to produce siderophores. Various strains, particularly endophytes, were able to grow on ACC as the sole nitrogen source.
478 citations
Cites background from "Metal Ion Recognition in Ligands wi..."
...They show high affinity for ferric iron but also form complexes with bivalent heavy metal ions (16) that can be assimilated by the plant....
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TL;DR: It is concluded that hydroxamate siderophores contained in culture filtrates of S. acidiscabies E13 promoted cowpea growth under nickel contamination by binding iron and nickel, thus playing a dual role of sourcing iron for plant use and protecting against nickel toxicity.
Abstract: The siderophore-producing ability of nickel-resistant Streptomyces acidiscabies E13 and the role of the elicited siderophores in promoting plant growth under iron and nickel stress are described. S...
229 citations
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...…towards Fe, with association constants of KB &1030 and 1032 for the hydroxamates desferrioxamine B (DFOB) and E (DFOE), respectively (Crumbliss 1991; Fernández and Winkelmann 2005), siderophores can also chelate other metals with lower binding strength (Evers et al. 1989; Hernlem et al. 1996)....
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TL;DR: This work highlights the capabilities of MALDI-IMS and MS/MS network analysis to study interkingdom interactions and provides insight into the complex nature of polymicrobial metabolic exchange and biotransformations.
Abstract: In polymicrobial infections, microbes can interact with both the host immune system and one another through direct contact or the secretion of metabolites, affecting disease progression and treatment options. The thick mucus in the lungs of patients with cystic fibrosis is highly susceptible to polymicrobial infections by opportunistic pathogens, including the bacterium Pseudomonas aeruginosa and the fungus Aspergillus fumigatus. Unravelling the hidden molecular interactions within such polymicrobial communities and their metabolic exchange processes will require effective enabling technologies applied to model systems. In the present study, MALDI-TOF and MALDI-FT-ICR imaging mass spectrometry (MALDI-IMS) combined with MS/MS networking were used to provide insight into the interkingdom interaction between P. aeruginosa and A. fumigatus at the molecular level. The combination of these technologies enabled the visualization and identification of metabolites secreted by these microorganisms grown on agar. A complex molecular interplay was revealed involving suppression, increased production, and biotransformation of a range of metabolites. Of particular interest is the observation that P. aeruginosa phenazine metabolites were converted by A. fumigatus into other chemical entities with alternative properties, including enhanced toxicities and the ability to induce fungal siderophores. This work highlights the capabilities of MALDI-IMS and MS/MS network analysis to study interkingdom interactions and provides insight into the complex nature of polymicrobial metabolic exchange and biotransformations.
217 citations
Cites background from "Metal Ion Recognition in Ligands wi..."
...Because of its similar size and charge as iron, aluminum can also complex siderophores (43)....
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TL;DR: The spectral properties of PvdI are used to show that in addition to Fe(3+), this siderophore also chelates, but with lower efficiencies, all the 16 metals used in the authors' screening, which allow a better understanding of the behaviour of the PvDI uptake pathway in the presence of metals other than iron.
Abstract: Pyoverdine (PvdI) is the major siderophore secreted by Pseudomonas aeruginosa PAOI in order to get access to iron. After being loaded with iron in the extracellular medium, PvdI is transported across the bacterial outer membrane by the transporter, FpvAI. We used the spectral properties of PvdI to show that in addition to Fe(3+), this siderophore also chelates, but with lower efficiencies, all the 16 metals used in our screening. Afterwards, FpvAI at the cell surface binds Ag(+), Al(3+), Cd(2+), Co(2+), Cu(2+), Fe(3+), Ga(3+), Hg(2+), Mn(2+), Ni(2+) or Zn(2+) in complex with PvdI. We used Inductively Coupled Plasma-Atomic Emission Spectrometry to monitor metal uptake in P. aeruginosa: TonB-dependent uptake, in the presence of PvdI, was only efficient for Fe(3+). Cu(2+), Ga(3+), Mn(2+) and Ni(2+) were also transported into the cell but with lower uptake rates. The presence of Al(3+), Cu(2+), Ga(3+), Mn(2+), Ni(2+) and Zn(2+) in the extracellular medium induced PvdI production in P. aeruginosa. All these data allow a better understanding of the behaviour of the PvdI uptake pathway in the presence of metals other than iron: FpvAI at the cell surface has broad metal specificity at the binding stage and it is highly selective for Fe(3+) only during the uptake process.
214 citations
Cites background from "Metal Ion Recognition in Ligands wi..."
...The supernatant containing PvdI was filtered through a 0.22 mM nitrocellulose membrane and the final PvdI concentration was determined at OD400 (e = 19 000 M-1 cm-1)....
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...These molecules are characterized by a high affinity for ferric iron [Ka = 1043 and 1032 M-1 for enterobactin and pyoverdine respectively (Albrecht-Gary et al., 1994; Raymond et al., 2003)], but they will also form complexes with metals other than Fe3+, although with lower affinity (Hernlem et al., 1996; Neubauer et al., 2000)....
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...For example, the formation constants for complexes of hydroxamate siderophore desferrioxamine B with Ga3+, Al3+ and In3+ are between 10(20) and 10(28) M-1 (Evers et al., 1989) and those for pyoverdine with Zn2+, Cu2+ and Mn2+ are between 10(17) and 10(22) M-1 (Chen et al....
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...For example, the formation constants for complexes of hydroxamate siderophore desferrioxamine B with Ga3+, Al3+ and In3+ are between 1020 and 1028 M-1 (Evers et al., 1989) and those for pyoverdine with Zn2+, Cu2+ and Mn2+ are between 1017 and 1022 M-1 (Chen et al., 1994)....
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TL;DR: It is pointed out that, despite the high concentration of heavy metals present in its tissues, A. bertolonii harbors an endophytic bacterial flora showing a high genetic diversity as well as a high level of resistance to heavy metals that could potentially help plant growth and Ni hyperaccumulation.
Abstract: We report the isolation and characterization of endophytic bacteria, endemic to serpentine outcrops of Central Italy, from a nickel hyperaccumulator plant, Alyssum bertolonii Desv. (Brassicaceae). Eighty-three endophytic bacteria were isolated from roots, stems, and leaves of A. bertolonii and classified by restriction analysis of 16S rDNA (ARDRA) and partial 16S rDNA sequencing in 23 different taxonomic groups. All isolates were then screened for siderophore production and for resistance to heavy metals. One isolate representative of each ARDRA group was then tested for plant tissue colonization ability in sterile culture.Obtained results pointed out that, despite the high concentration of heavy metals present in its tissues, A. bertolonii harbors an endophytic bacterial flora showing a high genetic diversity as well as a high level of resistance to heavy metals that could potentially help plant growth and Ni hyperaccumulation.
192 citations
Cites background from "Metal Ion Recognition in Ligands wi..."
...Siderophores are organic molecules that show high affinity for Fe(III) ions, but they can also form complexes with other bivalent heavy metal ions [13] that can be assimilated by the plant....
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References
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TL;DR: The existence of linear relationships between the formation constant log values of complexes of ligands containing negative oxygen donor groups only and log K 1 (OH − ) values for the metal ions is demonstrated in this article.
Abstract: The existence of good linear relationships between the formation constant log values of complexes of ligands containing negative oxygen donor groups only and log K 1 (OH − ) values for the metal ions is demonstrated for a variety of ligands containing phenolate, carboxylate, and hydroxamate donor groups. The formation constants of DFB (desferriferrioxamine-B), BAMTPH (a synthetic trihydroxamate), and several dihydroxamic acids of the type HONHCO(CH 2 ) n CONHOH with several metal ions are reported and used to demonstrate the general existence of linear relationships of the above type
262 citations