Journal ArticleDOI
Metal–Organic Framework in Situ Post-Encapsulating DNA–Enzyme Composites on a Magnetic Carrier with High Stability and Reusability
Reads0
Chats0
TLDR
A novel and stable strategy for enzyme immobilization was designed by use of ZIF-8 to encapsulate in situ DNA-enzyme composites on the surface of magnetic particles (MP) and was proven to be a general platform that can be used to stabilize various proteins.Abstract:
In recent years, metal-organic frameworks (MOFs) have been extensively studied as candidate enzyme immobilization platforms. However, conventional MOF-enzyme composites usually exhibit low controllability and reusability. In this study, a novel and stable strategy for enzyme immobilization was designed by use of ZIF-8 to encapsulate in situ DNA-enzyme composites on the surface of magnetic particles (MPs). The mechanism of in situ encapsulation was discussed in detail. It was found that immobilized enzymes were involved in the growth of ZIF-8, and the DNA cross-linking agents promoted the growth of ZIF-8 on the surface of MP. The thermal, chemical, and physical stabilities of horseradish peroxidase (HRP) were all significantly enhanced after in situ encapsulation. Most importantly, this strategy was proven to be a general platform that can be used to stabilize various proteins. The in situ encapsulation strategy was expanded to immobilize a cascade of enzymes, and ZIF-8@MPGOx-HRP possessed high selectivity and a wide linear range (25-500 μM) for glucose detection.read more
Citations
More filters
Journal ArticleDOI
Metal-organic framework composites as green/sustainable catalysts
TL;DR: In this article, the use of MOF composites as catalysts in several typical reactions including cycloadditions, CO2 insertions, cyanosilylations, and condensations is investigated.
Journal ArticleDOI
Single-atom platinum nanocatalyst-improved catalytic efficiency with enzyme-DNA supermolecular architectures
Ruijin Zeng,Weijun Wang,Guoneng Cai,Zhenliang Huang,Jiangming Tao,Dianping Tang,Chengzhou Zhu +6 more
TL;DR: In this article, a SiO2-templated strategy was designed to synthesize single-atom platinum anchored on the surface of hollow cadmium sulfide (HCdS-Pt1).
Journal ArticleDOI
Metal-Organic Frameworks Enhance Biomimetic Cascade Catalysis for Biosensing.
TL;DR: In this article, metal-organic frameworks (MOFs) with porous structures, unique nano/microenvironments, and good biocompatibility have been skillfully used as carriers to immobilize enzymes for shielding them against hash surroundings and improving the catalytic efficiency.
Journal ArticleDOI
Three-dimensional ordered magnetic macroporous metal-organic frameworks for enzyme immobilization.
Yuxiao Feng,Hongtong Hu,Zichen Wang,Yingjie Du,Le Zhong,Chenxi Zhang,Yanjun Jiang,Shiru Jia,Jiandong Cui +8 more
TL;DR: In this paper, a novel magnetic amino-functionalized zeolitic imidazolate framework-8 (ZIF-8) with 3D highly ordered macroporous structure was synthesized using the assembled polystyrene (PS) nanosphere monoliths as a template.
Journal ArticleDOI
Recent advances in enzyme immobilization based on novel porous framework materials and its applications in biosensing
TL;DR: In this paper , the authors discuss various strategies of using MOFs/functional MOFs as matrixes to immobilize enzymes, and the latest research progresses of COFs and HOFs in enzyme immobilization are systematically introduced.
References
More filters
Journal ArticleDOI
Exceptional chemical and thermal stability of zeolitic imidazolate frameworks
Kyo Sung Park,Zheng Ni,Adrien P. Côté,Jae Yong Choi,Rudan Huang,Fernando J. Uribe-Romo,Hee K. Chae,Michael O'Keeffe,Omar M. Yaghi +8 more
TL;DR: Study of the gas adsorption and thermal and chemical stability of two prototypical members, ZIF-8 and -11, demonstrated their permanent porosity, high thermal stability, and remarkable chemical resistance to boiling alkaline water and organic solvents.
Journal ArticleDOI
Industrial biocatalysis today and tomorrow
TL;DR: Biocatalytic processes can now be carried out in organic solvents as well as aqueous environments, so that apolar organic compounds aswell as water-soluble compounds can be modified selectively and efficiently with enzymes and bioc atalytically active cells.
Journal ArticleDOI
Engineering the third wave of biocatalysis
Uwe T. Bornscheuer,Gjalt W. Huisman,Romas J. Kazlauskas,Romas J. Kazlauskas,Stefan Lutz,Jeffrey C. Moore,Karen Robins +6 more
TL;DR: Applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step are discussed.
Journal ArticleDOI
Synthetic protein scaffolds provide modular control over metabolic flux
John E. Dueber,Gabriel C. Wu,G Reza Malmirchegini,G Reza Malmirchegini,Tae Seok Moon,Christopher J. Petzold,Christopher J. Petzold,Adeeti Ullal,Kristala L. J. Prather,Jay D. Keasling +9 more
TL;DR: In this article, synthetic protein scaffolds bearing interaction domains from metazoan signaling proteins were used to spatially recruit metabolic enzymes in a designable manner, and the modularity of these domains enabled them to optimize the stoichiometry of three mevalonate biosynthetic enzymes recruited to a synthetic complex.
Journal ArticleDOI
Industrial use of immobilized enzymes
TL;DR: The development and attributes of several established and emerging industrial applications for immobilized enzymes, including high-fructose corn syrup production, pectin hydrolysis, debittering of fruit juices, interesterification of food fats and oils, biodiesel production, and carbon dioxide capture are reviewed herein, highlighting factors that define the advantages of enzyme immobilization.