Methane production by mixed ruminal cultures incubated in dual-flow fermentors.

doi:10.3168/JDS.S0022-0302(04)73148-3

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Methane production by mixed ruminal cultures incubated in dual-flow fermentors.

Journal Article•DOI•

Methane production by mixed ruminal cultures incubated in dual-flow fermentors.

Jong-Su Eun¹, V. Fellner¹, Marcia L. Gumpertz¹•Institutions (1)

North Carolina State University¹

01 Jan 2004-Journal of Dairy Science (Elsevier)-Vol. 87, Iss: 1, pp 112-121

TL;DR: Dilution rate and forage-to-concentrate ratio altered the partition of substrate by microbes and underestimated methane output at higher dilution rates and with high forage diets.

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About: This article is published in Journal of Dairy Science.The article was published on 2004-01-01 and is currently open access. It has received 70 citations till now. The article focuses on the topics: Dilution.

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Journal Article•DOI•

The effect of the diet fed to donor sheep on in vitro methane production and ruminal fermentation of diets of variable composition

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M. E. Martínez¹, M. E. Martínez², M. J. Ranilla¹, M. J. Ranilla², María L. Tejido², María L. Tejido¹, Cristina Saro¹, Cristina Saro², María Dolores Carro², María Dolores Carro¹ - Show less +6 more•Institutions (2)

University of León¹, Spanish National Research Council²

23 Jun 2010-Animal Feed Science and Technology

TL;DR: The results indicate that methane production in vitro is affected by both F:C ratio and type of forage in the diet of donors, and these variables should be taken into account when conducting in vitro experiments.

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47 citations

Journal Article•DOI•

Effects of forage legumes containing condensed tannins on methane and ammonia production in continuous cultures of mixed ruminal microorganisms

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C. M. Williams¹, Jong-Su Eun¹, Jennifer W. MacAdam¹, Allen J. Young¹, V. Fellner², B. R. Min³ - Show less +2 more•Institutions (3)

Utah State University¹, North Carolina State University², Tuskegee University³

23 Jun 2011-Animal Feed Science and Technology

TL;DR: In this paper, the effects of condensed tannin (CT) containing legume hays in dairy total mixed rations (TMR) on ruminal fermentation, with an emphasis on CH4 and ammonia N production, were investigated.

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41 citations

Journal Article•DOI•

Influence of disodium malate on microbial growth and fermentation in rumen-simulation technique fermenters receiving medium- and high-concentrate diets.

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J. A. Gómez¹, M. L. Tejido¹, María Dolores Carro¹•Institutions (1)

University of León¹

01 Apr 2005-British Journal of Nutrition

TL;DR: If these results are confirmed in vivo, malate could be used as a feed additive for ruminants fed diets containing medium proportions of forage and not only in animals fed high-concentrate diets, as has so far been proposed.

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Abstract: Two incubation trials were carried out with the rumen-simulation technique (RUSITEC). In each trial, four vessels received a diet of grass hay and concentrate (600 and 400 g/kg DM, respectively, diet F), and the other four were fed a diet composed of concentrate and barley straw (900 and 100 g/kg DM, respectively; Diet C). Vessels were given 20 g of the corresponding diet daily, and half of them were supplemented with disodium malate to achieve a final concentration of 6.55 mM. There were no effects (P>0.05) of malate either on pH or on the daily production of NH 3 -N, but malate treatment increased (P 0.05) were observed for diet C. For both diets, there were no differences (P>0.05) between treatments in the daily flow of liquid-associated micro-organisms measured using 15 N as a microbial marker. These results indicate that malate stimulated the in vitro fermentation of both diets by increasing the apparent disappearance of the diet and decreasing the ratio of CH 4 :volatile fatty acids, but a greater response was observed with diet F. If these results are confirmed in vivo, malate could be used as a feed additive for ruminants fed diets containing medium proportions of forage (i.e. dairy animals) and not only in animals fed high-concentrate diets, as has so far been proposed.

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39 citations

Cites result from "Methane production by mixed ruminal..."

...Our values were in the range of those previously reported in continuous fermenters fed diets differing in their forage:concentrate ratio and maintained at a pH of less than 6·3 (Eun et al. 2004)....
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Journal Article•DOI•

Evaluation of a stratified continuous rumen incubation system

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S. Muetzel¹, S. Muetzel², Peter Lawrence¹, E. Hoffmann¹, Klaus Becker¹ - Show less +1 more•Institutions (2)

University of Hohenheim¹, AgResearch²

12 May 2009-Animal Feed Science and Technology

TL;DR: A continuous culture system based on a naturally compartmented rumen simulation system for the continuous culture of rumen bacteria and protozoa has potential to study effects of feed components on fermentation parameters and is suitable for modelling changes in the composition of the rumen microbial community.

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37 citations

Journal Article•DOI•

Changes in methane emission, rumen fermentation in response to diet and microbial interactions

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Sanjay Kumar¹, Sumit Singh Dagar¹, Anil Kumar Puniya¹, Ramesh C. Upadhyay¹•Institutions (1)

National Dairy Research Institute¹

01 Apr 2013-Research in Veterinary Science

TL;DR: To evaluate relative contributions of different microbial groups in rumen, the mono-culture and co-cultures were tested in vitro using high and low roughage diets and Digestibility and total volatile fatty acids were significantly lowered with protozoal Mono-culture.

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36 citations

Cites background from "Methane production by mixed ruminal..."

...Similarly, Eun et al. (2004) reported highest methane production on high forage (70...
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References

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Principles and procedures of statistics.

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R. G. D. Steel, J. H. Torrie

01 Jan 1960

23,037 citations

Journal Article•DOI•

Principles and Procedures of Statistics.

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F. N. David, R. G. D. Steel, J. H. Torrie

01 Jun 1961-Biometrika

21,056 citations

Book•

Nutritional Ecology of the Ruminant

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Peter J. Van Soest

12 Aug 1994

9,191 citations

"Methane production by mixed ruminal..." refers background or methods in this paper

...The amount of substrate fermented to VFA, CH4, and CO2 was calculated based on the moles of individual VFA produced, daily methane output, and CO2 released from fermentation and buffer addition (Wolin, 1960; Van Soest, 1994; Blümmel et al., Journal of Dairy Science Vol. 87, No. 1, 2004 1997)....
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...Increased lysis of microbial cells as a consequence of substrate exhaustion and uncoupled fermentation may contribute to reduced net growth at longer incubation times (Van Nevel and Demeyer, 1977)....
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...Also, total gas yield can vary considerably due to the incorporation of carbons into microbial mass as well as the different metabolic pathways by which carbohydrate fractions can be degraded by rumen microbes (Krishnamoorthy et al., 1991; Beuvink and Spoelstra, 1992; Van Soest, 1994)....
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...It is known that the growth yields of ruminal microbes can be relatively high, and that microbial cells have a negative oxidation-reduction state (Van Kessel and Russell, 1996)....
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...Stoichiometric equations relating substrate degradation to VFA and gas production have been developed and are commonly used to estimate digestibility of ruminant feeds (Wolin, 1960; Russell and Baldwin, 1979; Menke et al., 1979; Van Soest, 1994)....
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Journal Article•DOI•

Estimación lineal de los requerimientos nutricionales del NRC para ganado de leche

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Jorge Elizondo¹•Institutions (1)

University of Costa Rica¹

20 Feb 2014-Agronomía Mesoamericana

TL;DR: Linear regression equations have been obtained to directly calculate the nutrient requirements of dairy cattle (TDN, DE, ME, NEL,CP, Ca, P, Vitamin A and Vitamin D) on different physiological stages: maintenance, pregnancy and milk production based on NRC nutrient requirements tables.

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Abstract: Linear regression equations have been obtained to directly calculatenutrient requirements of dairy cattle (TDN, DE, ME, NEL,CP, Ca, P, Vitamin A and Vitamin D) on differentphysiological stages: maintenance, pregnancy and milkproduction based on NRC nutrient requirements tables. TheR-square was calculated for each equation to establish thedegree of adjustment.

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6,663 citations

Journal Article•DOI•

A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability.

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Charles J. Sniffen¹, J D O'Connor¹, P.J. Van Soest¹, Danny G. Fox¹, James B. Russell¹, James B. Russell², James B. Russell³ - Show less +3 more•Institutions (3)

Cornell University¹, Ithaca College², United States Department of Agriculture³

01 Nov 1992-Journal of Animal Science

TL;DR: The Cornell Net Carbohydrate and Protein System has a submodel that predicts rates of feedstuff degradation in the rumen, the passage of undegraded feed to the lower gut, and the amount of ME and protein that is available to the animal.

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Abstract: The Cornell Net Carbohydrate and Protein System (CNCPS) has a submodel that predicts rates of feedstuff degradation in the rumen, the passage of undegraded feed to the lower gut, and the amount of ME and protein that is available to the animal. In the CNCPS, structural carbohydrate (SC) and nonstructural carbohydrate (NSC) are estimated from sequential NDF analyses of the feed. Data from the literature are used to predict fractional rates of SC and NSC degradation. Crude protein is partitioned into five fractions. Fraction A is NPN, which is trichloroacetic (TCA) acid-soluble N. Unavailable or protein bound to cell wall (Fraction C) is derived from acid detergent insoluble nitrogen (ADIP), and slowly degraded true protein (Fraction B3) is neutral detergent insoluble nitrogen (NDIP) minus Fraction C. Rapidly degraded true protein (Fraction B1) is TCA-precipitable protein from the buffer-soluble protein minus NPN. True protein with an intermediate degradation rate (Fraction B2) is the remaining N. Protein degradation rates are estimated by an in vitro procedure that uses Streptomyces griseus protease, and a curve-peeling technique is used to identify rates for each fraction. The amount of carbohydrate or N that is digested in the rumen is determined by the relative rates of degradation and passage. Ruminal passage rates are a function of DMI, particle size, bulk density, and the type of feed that is consumed (e.g., forage vs cereal grain).

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3,354 citations