Methane production from protozoan endosymbionts following stimulation of microbial metabolism within subsurface sediments
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Cites background from "Methane production from protozoan e..."
...The methanogenic Archaea were not directly affected by the diet, however, for methanogenesis they rely on metabolites produced by other community members (presumably a member of the Clostridiales in this case), which were decreased due to 15 the change in diet....
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...directly affected, and this has been observed before in a different setting with intracellular Archaea [465]....
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Cites background from "Methane production from protozoan e..."
...General cellular processes, like replication, in which the cytoskeleton is involved, will then probably be directly affected, and this has been observed before in a different setting with intracellular Archaea (Holmes et al., 2014)....
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...Closer inspection revealed that with an increase of CS in the diet, nearly all genes of the methanogenesis pathways were downregulated in a subset of the Archaea (expression profile C)....
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...These groups included 13 genera (Bacteroides, Butyrivibrio, Clostridium, Entamoeba, Entodinium, Eubacterium, Faecalibacterium, Fibrobacter, Methanobrevibacter, Methanosphaera, Plasmodium, Prevotella, Ruminococcus) and 11 sequence clusters (not including the data assigned to the 13 genera) that could only be assigned at higher taxonomic levels (Archaea, Bacteria, Bacteroidales, Bacteroidetes, Clostridia, Clostridiales, Coriobacteriaceae, Eukaryota, Firmicutes, Methanobacteriaceae, Peptostreptococcaceae)....
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...Furthermore one of these modules was found in the Archaea, which are not known to harbour either cellulosomal complexes or their starch counterparts....
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...As several Archaea are endosymbionts of Protozoa, it can be speculated that an experimental change, which has an impact on the symbionts, will also affect their host (Finlay et al., 1994) (although this relationship is also not entirely clear (Morgavi et al., 2012))....
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"Methane production from protozoan e..." refers methods in this paper
...Alignments were made in ClustalX (Thompson et al., 1997) and corrected with ProSeq v2....
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37,956 citations
"Methane production from protozoan e..." refers methods in this paper
...9 (Filatov, 2002) before phylogenetic trees were constructed with Mega v6 (Tamura et al., 2013)....
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"Methane production from protozoan e..." refers methods in this paper
...Transcript abundances and qPCR efficiencies (90–99%) were calculated from appropriate standard curves and all qPCR experiments followed MIQE guidelines (Bustin et al., 2009)....
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...%) were calculated from appropriate standard curves and all qPCR experiments followed MIQE guidelines (Bustin et al., 2009)....
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