Patent•
Method for breeding salt-resistant tomato utilizing atriplex hortensis BADH gene transfer
22 Oct 2003-
TL;DR: In this paper, a method for cultivating salt-enduring tomato line by transferring betaine aldehyde dehydrogenase gene (BADH) includes the following steps: a. Using Agrobacterium LBA 4404 of double-element expression vector pBin438 of gene fragment containing BADH cDNA to infect tomato euphylis blade, b. placing the infected tomato blade on the IM culture medium to make differentiation and culture, c. extracting genome DNA from teneral blade of differentiated plant to make molecular detection and d.
Abstract: The method for cultivating salt-enduring tomato line by transferring betaine aldehyde dehydrogenase gene (BADH) includes the following steps: a. using Agrobacterium LBA 4404 of double-element expression vector pBin438 of gene fragment containing BADH cDNA to infect tomato euphylis blade, b. placing the infected tomato blade on the IM culture medium to make differentiation and culture, c. extracting genome DNA from teneral blade of differentiated plant to make molecular detection and d. BADH activity detection and salt-enduring property determination of transgenic plant.
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Patent•
23 Feb 2005
TL;DR: A transgenic and tissue culture method for quickly reproducing the tetraploid locust tree includes such steps as sampling the new stem of the tree, disinfecting, starting culturing, inducing culture to obtain calli, transgenic treating by Agrobacterium tumefaciens LBA 4404 containing binary carrier pBin 438, differential culturing and selection culturing to obtain resistant plant, molecular testing to obtain transgenic plant, rooting culture, naturalizing and planting in field as mentioned in this paper.
Abstract: A transgenic and tissue culture method for quickly reproducing the tetraploid locust tree includes such steps as sampling the new stem of tetraploid locust tree, disinfecting, start culturing, inducing culture to obtain calli, transgenic treating by Agrobacterium tumefaciens LBA 4404 containing binary carrier pBin 438, differential culturing, selection culturing to obtain resistant plant Kan, molecular testing to obtain transgenic plant, rooting culture, naturalizing and planting in field.
6 citations
Patent•
28 Jul 2010
TL;DR: In this article, a gene transformation method of oil sunflower, which comprises the following steps of (1) taking the tissue of a test tube plantlet of the robustly-growth oil-sunflower as an explant; (2) inoculating one single bacterial colony of agrobacterium tumfaciens C58C1 into an LB liquid nutrient medium for shake cultivation overnight, taking out the bacterial colony after the OD value is 0.2-0.6 and impregnating the explant of the plantlet, and (3) placing the impregnated
Abstract: The invention relates to a gene transformation method of oil sunflower, which comprises the following steps of (1) taking the tissue of a test tube plantlet of the robustly-growth oil sunflower as an explant; (2) inoculating one single bacterial colony of agrobacterium tumfaciens C58C1 into an LB liquid nutrient medium for shake cultivation overnight, taking out the bacterial colony after the OD value is 0.2-0.6 and impregnating the explant of the tissue of the test tube plantlet of the oil sunflower; (3) placing the impregnated explant of the tissue of the test tube plantlet of the oil sunflower on a differential medium containing 2-5mg/L of herbicide for differential cultivation for sub-culturing every time within 0-14 days so as to generate an inversion plantlet; (4) directly inverting the inversion plantlet screened by the herbicide into a rooting medium and transplanting the inversion plantlet into a flower pot when the inversion plantlet grows at 2-5cm. The invention establishes the set of the gene transformation method of the oil sunflower, so as to lead the steps of screening the marking concentration (the herbicide), choosing different inversion explants and adopting different sub-culturing times to be clear respectively and obtain the highest inversion efficiency, thereby laying the foundation for various gene transformations of the oil sunflower.
1 citations
Patent•
11 Nov 2010
TL;DR: Mangrove Betaine aldehyde dehydrogenase gene and uses thereof in the researches of breeding new plant species having salt, drought and low temperature tolerances are provided as discussed by the authors.
Abstract: Mangrove Betaine aldehyde dehydrogenase gene and the uses thereof in the researches of breeding new plant species having salt, drought and low temperature tolerances are provided. The mangrove betaine aldehyde dehydrogenase gene has the nucleotide sequence as shown in SEQ ID NO: 1. Utilizing the said mangrove betaine aldehyde dehydrogenase gene to construct prokaryotic expression vectors and binary plant expression vectors, transforming E.coli and tobacco respectively with the vectors, culturing BADH transgenic E.coli under high salt conditions, and conducting abiotic stress experiments on BADH positive transgenic tobacco. The mangrove betaine aldehyde dehydrogenase gene is highly expressed in E.coli, and under high salt conditions, the growth of BADH transgenic E.coli is better than non-transgenic E.coli. Transgenic tobacco identification results show that the mangrove betaine aldehyde dehydrogenase gene cloned can be highly expressed in tobacco, and the transgenic tobacco obtained has good ability to tolerate some abiotic stress.
Patent•
01 May 2013
TL;DR: In this article, a method for irrigating transgenic tomatoes by brine is described, in which the brine in the water quantity required by the normal growth of the tomatoes is used for irrigation.
Abstract: The invention discloses a method for irrigating transgenic tomatoes by brine. The method is characterized in that the brine in the water quantity required by the normal growth of the tomatoes is used for irrigating the transgenic tomatoes in one kind or two kinds in the following two periods (a) and (b), and fresh water in the water quantity required by the normal growth of the tomatoes in other breeding periods is used for irrigating the transgenic tomatoes, in the first fruit expansion period (a), the first fruit diameter reaches 2cm until the second fruit diameter reaches 2cm, in the second fruit expansion period (b), the fruit diameter reaches 2cm until the first fruit is harvested, the transgenic tomatoes are salt tolerant gene expression tomatoes obtained by guiding salt tolerant genes into the target tomatoes, and the brine is 200mM NaCl water solution. The method has the advantages that genetic transformation is combined with irrigation measures, and results show that after 200mM NaCl is irrigated in the first fruit expansion period or the second fruit expansion period, the yield of the transgenic tomatoes is not reduced through being compared with that of the normal grown tomatoes, and the method has important use values in the tomato production.