Patent
Method of using a taq dna polymerase without 5'-3'-exonuclease activity
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TLDR
In this paper, a modified Taq DNA polymerase and methods for its use are described and a modified version of the original Taq polymerase is presented. But the present invention is directed to a modified DNA polymerization.Abstract:
The present invention is directed to a modified Taq DNA polymerase and methods for its use.read more
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Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
TL;DR: In this article, the authors used the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide fragments for detection.
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Methods and apparatuses for analyzing polynucleotide sequences
TL;DR: In this article, a high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention of the algorithm.
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Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
Albert Carmelo Mossa,Lisa May Goven,John G. Atwood,Fenton Williams,Timothy M. Woudenberg,Marcel Margulies,Robert Ragusa,Richard Leath +7 more
TL;DR: In this paper, an instrument for performing highly accurate PCR employing a sample block in microtiter tray format is described. But the sample block has local balance and local symmetry, and the control software includes diagnostics.
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Short cycle methods for sequencing polynucleotides
TL;DR: In this article, a polynucleotide sequence is sequenced by stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion, which is called stopping the cycle.
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Process for direct sequencing during template amplification
TL;DR: Processes and kits for simultaneously amplifying and sequencing nucleic acid molecules, and performing high throughput DNA sequencing are described in this article, where the authors also present a high throughput sequencing algorithm.
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Purified thermostable enzyme
TL;DR: In this article, the authors used recombinant DNA sequences encoding a thermostable DNA polymerase from Thermus aquaticus to produce a recombinant protein with a molecular weight of about 86,000-95,000 daltons.
Patent
Purified thermostable enzyme and process for amplifying, detecting, and/or cloning nucleic acid sequences using said enzyme
Henry Anthony Erlich,Glenn Thomas Horn,Randall Keichi Saiki,Susanne Stoffel,Kary B. Mullis,Frances Cook Lawyer,David H. Gelfand +6 more
TL;DR: A purified thermostable enzyme is obtained that has unique characteristics as mentioned in this paper, which can be used in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates.