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Journal ArticleDOI

Microbial and enzymatic methods for the removal of caffeine

TL;DR: Development of a process involving an enzymatic (specific) degradation of caffeine to non-toxic compound is necessary to solve the problems of chemical extraction of caffeine in food products as well as treating the caffeine containing waste products.
About: This article is published in Enzyme and Microbial Technology.The article was published on 2005-07-01. It has received 127 citations till now. The article focuses on the topics: Decaffeination & Caffeine.
Citations
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Journal ArticleDOI
TL;DR: It is shown that caffeine is degraded in the gut of H. hampei, and that experimental inactivation of the gut microbiota eliminates this activity, and Pseudomonas caffeine demethylase genes are expressed in vivo in the intestine confirming their key role.
Abstract: The coffee berry borer (Hypothenemus hampei) is the most devastating insect pest of coffee worldwide with its infestations decreasing crop yield by up to 80%. Caffeine is an alkaloid that can be toxic to insects and is hypothesized to act as a defence mechanism to inhibit herbivory. Here we show that caffeine is degraded in the gut of H. hampei, and that experimental inactivation of the gut microbiota eliminates this activity. We demonstrate that gut microbiota in H. hampei specimens from seven major coffee-producing countries and laboratory-reared colonies share a core of microorganisms. Globally ubiquitous members of the gut microbiota, including prominent Pseudomonas species, subsist on caffeine as a sole source of carbon and nitrogen. Pseudomonas caffeine demethylase genes are expressed in vivo in the gut of H. hampei, and re-inoculation of antibiotic-treated insects with an isolated Pseudomonas strain reinstates caffeine-degradation ability confirming their key role.

305 citations

Journal ArticleDOI
20 Jun 2018-Genes
TL;DR: In this paper, the authors discuss the recent literature on the production of representatives of three plant secondary metabolite classes: artemisinin (a sesquiterpene), lignans (phenolic compounds) and caffeine (an alkaloid).
Abstract: Plants are sessile organisms and, in order to defend themselves against exogenous (a)biotic constraints, they synthesize an array of secondary metabolites which have important physiological and ecological effects. Plant secondary metabolites can be classified into four major classes: terpenoids, phenolic compounds, alkaloids and sulphur-containing compounds. These phytochemicals can be antimicrobial, act as attractants/repellents, or as deterrents against herbivores. The synthesis of such a rich variety of phytochemicals is also observed in undifferentiated plant cells under laboratory conditions and can be further induced with elicitors or by feeding precursors. In this review, we discuss the recent literature on the production of representatives of three plant secondary metabolite classes: artemisinin (a sesquiterpene), lignans (phenolic compounds) and caffeine (an alkaloid). Their respective production in well-known plants, i.e., Artemisia, Coffea arabica L., as well as neglected species, like the fibre-producing plant Urtica dioica L., will be surveyed. The production of artemisinin and caffeine in heterologous hosts will also be discussed. Additionally, metabolic engineering strategies to increase the bioactivity and stability of plant secondary metabolites will be surveyed, by focusing on glycosyltransferases (GTs). We end our review by proposing strategies to enhance the production of plant secondary metabolites in cell cultures by inducing cell wall modifications with chemicals/drugs, or with altered concentrations of the micronutrient boron and the quasi-essential element silicon.

181 citations


Cites background from "Microbial and enzymatic methods for..."

  • ...Different microbial platforms can also be used for the characterization of novel enzymes for the bioremediation of xanthine-derived compounds, such as degrading caffeine in wastewater [124]....

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Journal ArticleDOI
TL;DR: Caffeine and EGCG (epigallocatechin gallate) were extracted from green tea using supercritical carbon dioxide (SCCO 2 ) with water as a cosolvent.

116 citations

Journal ArticleDOI
TL;DR: Development of biodecaffeination techniques using these enzymes or using whole cells offers an attractive alternative to the present existing chemical and physical methods removal of caffeine, which are costly, toxic and non-specific to caffeine.
Abstract: Catabolism of caffeine (1,3,7-trimethylxanthine) in microorganisms commences via two possible mechanisms: demethylation and oxidation. Through the demethylation route, the major metabolite formed in fungi is theophylline (1,3-dimethylxanthine), whereas theobromine (3,7-dimethylxanthine) is the major metabolite in bacteria. In certain bacterial species, caffeine has also been oxidized directly to trimethyl uric acid in a single step. The conversion of caffeine to its metabolites is primarily brought about by N-demethylases (such as caffeine demethylase, theobromine demethylase and heteroxanthinedemethylase), caffeine oxidase and xanthine oxidase that are produced by several caffeine-degrading bacterial species such as Pseudomonas putida and species within the genera Alcaligenes, Rhodococcus and Klebsiella. Development of biodecaffeination techniques using these enzymes or using whole cells offers an attractive alternative to the present existing chemical and physical methods removal of caffeine, which are costly, toxic and non-specific to caffeine. This review mainly focuses on the biochemistry of microbial caffeine degradation, presenting recent advances and the potential biotechnological application of caffeine-degrading enzymes.

105 citations


Cites background or methods from "Microbial and enzymatic methods for..."

  • ...The degradation pathway in filamentous fungi is different and species from various fungal genera, e.g. Penicillium, Aspergillus, Stemphylium, Rhizopus, Phanerochaete, Trichoderma and Fusarium, have been shown to catabolize caffeine (Mazzafera 2002; Gokulakrishnan et al. 2005)....

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  • ...The potential use of microorganisms and enzymes obtained from microbial system for developing biological decaffeination techniques offer a much attractive alternative to the present existing techniques (Gokulakrishnan et al. 2005)....

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  • ...Caffeine enters the human system in the form of beverages like tea, coffee and caffeinated soft drinks and numerous food products like chocolates and desserts, with the global consumption ranging from 80 to 400 mg caffeine per person per day (Gokulakrishnan et al. 2005)....

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  • ...Penicillium, Aspergillus, Stemphylium, Rhizopus, Phanerochaete, Trichoderma and Fusarium, have been shown to catabolize caffeine (Mazzafera 2002; Gokulakrishnan et al. 2005)....

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Journal ArticleDOI
TL;DR: In this paper, the supercritical carbon dioxide (SC-CO2) was used for decaffeination of green tea leaves, which is known to be an ideal solvent, coupled with a cosolvent, such as ethanol or water.

94 citations

References
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Book
01 Jan 1977
TL;DR: The Plant and Its Biochemical Adaptation to the Environment, and Higher Plant-Lower Plant Interactions: Phytoalexins and Phytotoxins.
Abstract: The Plant and Its Biochemical Adaptation to the Environment. Biochemistry of Plant Pollination. Plant Toxins and Their Effects on Animals. Hormonal Interactions Between Plants and Animals. Insect Feeding Preferences. Feeding Preferences of Vertebrates, Including Man. The Co-Evolutionary Arms Race: Plant Defence and Animal Response. Animal Pheromones and Defence Substances. Biochemical Interactions Between Higher Plants. Higher Plant-Lower Plant Interactions: Phytoalexins and Phytotoxins. Indices.

1,368 citations

Journal ArticleDOI
TL;DR: The Co-Evolutionary Arms Race: Plant Defence and Animal Response as mentioned in this paper The Plant and its Biochemical Adaptation to the Environment The plant and its biochemical adaptation to the environment.
Abstract: The Plant and Its Biochemical Adaptation to the Environment. Biochemistry of Plant Pollination. Plant Toxins and Their Effects on Animals. Hormonal Interactions Between Plants and Animals. Insect Feeding Preferences. Feeding Preferences of Vertebrates, Including Man. The Co-Evolutionary Arms Race: Plant Defence and Animal Response. Animal Pheromones and Defence Substances. Biochemical Interactions Between Higher Plants. Higher Plant-Lower Plant Interactions: Phytoalexins and Phytotoxins. Indices.

750 citations

Book
01 Jun 1976
TL;DR: In this paper, the authors present a Microactivity Test for Fluid Catalytic Cracking Catalyst Performance (MCTC) for measuring the effect of catalytic cracking on the performance of flow-sensitive fluid systems.
Abstract: "Methanol from Coal, Cost Projections, R. I. Kermode, A. F. Nicholson, and J. E. Jones, Jr. Methanol as Fuel, Economics, Laurence H. Cohen and Herman L. Muller Methanol Synthesis: Trickle Flow Reactor, K. R. Westerterp and M. Kuczynski Methyl Ethyl Ketone, James J. Baiel, Charles Savini, and Jon E. R. Stanat Methyl Isobutyl Ketone, Austin G. Habib Methyl Methacrylate, R. V. Porcelli and B. Juran Methyl tertiary-Butyl Ether, L. S. Bitar, E. A. Hazbun, and W. J. Piel Mica Supply-Demand Relationships, edited by John J. McKetta Microactivity Test for Fluid Catalytic Cracking Catalyst Performance, Raymond W. Mott Microelectronics, Graydon B. Larrabee Microemulsions, Syed Qutubuddin Microencapsulation, Robert E. Sparks Microprocessors, Use in Distributed Control Systems, Gabriel J. Cordova, Herold I. Hertanu, and George T. Doyle Microwave Technology and Applications, Robert F. Schiffmann Minerals, Nonfuel, Economic Conditions, edited by John J. McKetta Mist Removal Equipment, Design and Selection, Timothy L. Holmes and Gilbert K. Chen Mixing: Agitation Intensity and Scale-up of Flow-Sensitive Fluid Systems, Richard L. Bowen, Jr. Mixing and Blending, James Y. Oldshue Mixing and Blending, Scale-up of Equipment, Adam Zanker Mixing: Flow and Shear Rates for Radial Turbines, Richard L. Bowen, Jr. Mixing, Static, Michael Mutsakis, F. A. Streiff, and G. Schneider Modeling Chemical Reactors, Robert W. Wansbrough Modular Design, M. A. Tan, R. P. Kumar, and G. Kuilanoff Modular Use in a Saudi Petrochemical Project, Jack B. Kirven and C. Ronald Swenson Moisture Condensation, Prevention of, John D. Constance Moisture Removal from Building Air, John D. Constance Molecular Sievies, Silicon Enriched, G. N. Long, R. L. Chiang, R. J. Pellet, and J. A. Rabo Molybdenum and Molybdenum Alloys Supply-Demand Relationships, edited by John J. McKetta Mothballing, Corrosion Prevention, Ronald J. Twigg Motivation, Lewis R. Timberlake Motors, Electric, John J. McKetta "

673 citations

Journal ArticleDOI
TL;DR: This article corrects the article on p. 403 in vol.
Abstract: [This corrects the article on p. 403 in vol. 40.].

514 citations

Journal ArticleDOI
TL;DR: In this article, a microwave-assisted extraction (MAE) method is presented for the extraction of tea polyphenols (TP) and tea caffeine from green tea leaves, and different solvents for the MAE procedure were investigated to optimize the extraction.
Abstract: A microwave-assisted extraction (MAE) method is presented for the extraction of tea polyphenols (TP) and tea caffeine from green tea leaves. Various experimental conditions. such as ethanol concentration (0 - 100%, v/v), MAE time (0.5 - 8 min), liquid/solid ratio (10:1 - 25:1 ml g(-1)), pre-leaching time (0-90 min) before MAE and different solvents for the MAE procedure were investigated to optimize the extraction, The extraction of tea polyphenols and tea caffeine with MAE for 4 min (30 and 4%) were higher than those of extraction at room temperature for 20 h. ultrasonic extraction for 90 min and heat reflux extraction for 45 min (28 and 3.6%), respectively. From the points of extraction time, the extraction efficiency and the percentages of tea polyphenols or tea caffeine in extracts, MAE was more effective than the conventional extraction methods studied. (C) 2002 Elsevier Science B.V. All rights reserved.

473 citations