Microbiological diagnosis of the severe chronic periodontitis
Lyudmila Boyanova,Lena Setchanova,Galina Gergova,Tomislav Kostyanev,Daniel Yordanov,Kamen Kotsilkov,Ivan Mitov +6 more
- Iss: 2009, pp 89-94
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TLDR
The presence of periodontopathogens as well as other bacterial species of possible importance should be considered in the patients with severe chronic periodontitis.Abstract:
In total, 14 adult patients with severe chronic periodontitis were evaluated for the presence of associated anaerobic and aerobic bacteria. Subgingival plaque specimens from three pocket depths per patient were obtained. Microaerophilic and facultative anaerobic bacteria, probably involved in the periodontitis, were isolated in six (42.9%) patients. These were Gram negative species involving Aggregatibacter (Haemophilus) aphrophilus (14.3%), Haemophilus parainfluenzae (7.1%), Kingella denitrificans (7.1%) and Moraxella osloensis (7.1%) as well as Gram-positive species, including Arcanobacterium (Actinomyces) pyogenes (7.1%) and Rhodococcus equi (7.1%). Anaerobic microbiology was completed for 12 patients. Of them, suspected periodontopathogens were isolated in seven (58.3%) patients and comprised Prevotella intermedia (in 41.7% of the patients) and Porphyromonas gingivalis (25%) as well as Porphyromonas endodontalis (8.3%). Tannerella forsythia was detected by PCR in half of the 12 cases. In conclusion, the presence of periodontopathogens as well as other bacterial species of possible importance should be considered in the patients with severe chronic periodontitis.read more
Citations
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Journal ArticleDOI
Subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis.
TL;DR: This review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment.
Journal ArticleDOI
Determinants and Interactions of Oral Bacterial and Fungal Microbiota in Healthy Chinese Adults
Man Kit Cheung,Jason Y. K. Chan,Martin C.S. Wong,Po Yee Wong,Pu Lei,Liuyang Cai,Lin-Lin Lan,Wendy C. S. Ho,Apple C.M. Yeung,Paul K.S. Chan,Zigui Chen +10 more
TL;DR: Different factors shape the diversity and structure of the oral bacterial and fungal microbial communities in healthy adults, with, for instance, sex and age only associated with the diversity of the bacterial community but not that of the fungal community.
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Prevalence of Anaerobic Bacteria in Periodontitis in Relation to Pocket Depth
TL;DR: In this study high prevalence of anaerobic bacteria in the periodontal pockets of patients suffering from periodontitis compared with the gingival sulcus of healthy subjects with marked shifting from mainly Gram positive facultative anaerilic bacteria in shallow pockets to mainly Gram-negative strictAnaerobicacteria in the deep pockets.
Journal ArticleDOI
The impact of the presence of Porphyromonas gingivalis on periodontal health in a group of patients with periodontitis in Erbil
TL;DR: This study investigated the detection rate of Porphyromonas gingivalis in chronic periodontitis patients versus healthy subjects using PCR assay, and its association with increased pocket depth and clinical attachment loss.
Journal ArticleDOI
Clinical and microbiological data in patients with chronic periodontitis
TL;DR: In this article, the authors showed that increasing levels of some specific pathogenic subgingival bacteria such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum, Prevotella intermedia and others can result in periodontal destruction and possibly correlate with disease severity.
References
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Journal ArticleDOI
New Bacterial Species Associated with Chronic Periodontitis
Purnima S. Kumar,Ann L. Griffen,J.A. Barton,Bruce J. Paster,Melvin L. Moeschberger,Eugene J. Leys +5 more
TL;DR: In this article, the purpose of the present investigation was to identify potential periodontal pathogens among newly identified species and phylotypes, and species-specific ribosomal 16S primers for PCR amplification were developed for detection of new species.
Journal ArticleDOI
Identification of Candidate Periodontal Pathogens and Beneficial Species by Quantitative 16S Clonal Analysis
TL;DR: Chronic periodontitis is the result of a global perturbation of the oral bacterial ecology rather than a disease-site specific microbial shift, and more differences were found in the bacterial profile between subjects withperiodontitis and healthy subjects than between deep and shallow sites within the same subject.
Journal ArticleDOI
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Isabela N. Rôças,Jr Jf Siqueira +1 more
TL;DR: The present findings using a large-scale analysis allowed the inclusion of some newly named species and as-yet-uncultivated phylotypes in the set of candidate pathogens associated with this disease.
Journal ArticleDOI
Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction
TL;DR: Rapid detection and quantification of periodontal pathogens in mouthwash samples are possible by real-time PCR, which is significantly less time-consuming than subgingival sampling with paper points.
Journal ArticleDOI
Identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions.
TL;DR: It is demonstrated that a wide range of oral species may be detected on or in crevicular epithelial cells from sites with periodontitis and from periodontally healthy sulci.
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