Microbiological diagnosis of the severe chronic periodontitis
26 Mar 2010-Iss: 2009, pp 89-94
TL;DR: The presence of periodontopathogens as well as other bacterial species of possible importance should be considered in the patients with severe chronic periodontitis.
Abstract: In total, 14 adult patients with severe chronic periodontitis were evaluated for the presence of associated anaerobic and aerobic bacteria. Subgingival plaque specimens from three pocket depths per patient were obtained. Microaerophilic and facultative anaerobic bacteria, probably involved in the periodontitis, were isolated in six (42.9%) patients. These were Gram negative species involving Aggregatibacter (Haemophilus) aphrophilus (14.3%), Haemophilus parainfluenzae (7.1%), Kingella denitrificans (7.1%) and Moraxella osloensis (7.1%) as well as Gram-positive species, including Arcanobacterium (Actinomyces) pyogenes (7.1%) and Rhodococcus equi (7.1%). Anaerobic microbiology was completed for 12 patients. Of them, suspected periodontopathogens were isolated in seven (58.3%) patients and comprised Prevotella intermedia (in 41.7% of the patients) and Porphyromonas gingivalis (25%) as well as Porphyromonas endodontalis (8.3%). Tannerella forsythia was detected by PCR in half of the 12 cases. In conclusion, the presence of periodontopathogens as well as other bacterial species of possible importance should be considered in the patients with severe chronic periodontitis.
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TL;DR: The study revealed that there are effects of age and sex on isolation rate and the results indicated that percentage of P.gingivalis was detected in 20-30 years old and males were more infected than females.
Abstract: The study aimed to detect P. gingivalis from 49 patients with periodontitis at different ages and both sexes, after determination of pocket depth, types of infection whether chronic or progressive by dentists. Routine culture method was done using selective media and anaerobic condition and compared with species specific polymerase chain reaction (PCR) technique. DNA was extracted from samples and its concentration and purity were determined. The results showed domination of chronic infections and the pocket depths ranged between 3-9mm, as well as the results revealed that isolation percent of P.gingivalis by PCR was more higher than culture method, it was 65.3% and 28.5% respectively. The results also showed that phenol-chloroform was the efficient method for DNA extraction comparing with other methods. The study revealed that there are effects of age and sex on isolation rate and the results indicated that percentage of P.gingivalis was detected in 20-30 years old and males were more infected than females.
2 citations
TL;DR: In this article , a 51-year-old female was admitted due to progressively worsening headache and left limb weakness for more than 10 days, and CT and MRI showed brain abscess.
2 citations
01 Jan 2014
TL;DR: The present study showed that 19 different species were responsible for the periodontal infections and the most common pathogens were staphylococcus saccharolyticus, streptococcus consellatus, and gemella morbillorum were the most most common anaerobicperiodontal pathogens isolated from patients in the present study.
Abstract: Background:Periodontal disease could be defined as a disorder of supporting structures of teeth, including the gingiva, periodontal ligament and alveolar bone. Periodontal disease develops from a preexisting gingivitis. However, not every case of gingivitis develops into a periodontal disease. The inflammation of gingiva alone is termed gingivitis, and the severe inflammation of the periodontal ligament with destruction of alveolar bone is called periodontal disease. Methods: One hundred eighteen samples were examined in the present study. Samples were obtained from periodontal pockets A plaque sample was inoculated onto different agar plates selected for anaerobic isolations. Periodontal pathogens were identified utilizing conventional methods, API RapID ANA system II , API 20A ,and Vitek2 ANC systems. Fifteen antibiotics were tested for their effectiveness on the various anaerobic bacteria isolated utilizing brucella blood agar plates disc diffusion methods. Results: peptostreptococcus prevotii , prevotella intermedia, prevotella melani , prevotella disiens, Bifidobacterium sp., Fusibacterium mortiferum peptostreptococcus tetradius ,and Wolinella sp. fusibacterium varium ,vellionella sp., campylobacter gracilis, capnocytophaga sp. ,peptostreptococcus magnus ,peptostreptococcus micros ,peptostreptococcus niger,peptostreptococcus anaerobius , staphylococcus saccharolyticus , streptococcus consellatus , and gemella morbillorum were the most common anaerobic periodontal pathogens isolated from patients in the present study.The bacteria isolated were generally highly sensitive to Imipeneme ,erythromcin , doxycycline, amoxycillin ,pipracillin, rodgyle ,spiromycin ,clindamycin , cephalexin ,cephalothin ,tetracycline and augmentin. Conclusion:The present study showed that 19 different species were responsible for the periodontal infections and the most common pathogens were
2 citations
TL;DR: Molecular method was able to detect Treponemal species even after scaling, however, in lower percentage than before it.
Abstract: The present study investigates the presence of four oral Treponemal species using rapid molecular methods. Subgingival fluid samples were obtained before and after one week of scaling and the nucleic acid was liberated from the bacterial cells to be used as a template for PCR. Specific primers for each Treponemal species targeted to16SrDNA sequence was depended.89% of samples were positive for Treponema sp., 88% of them were positive to the presence of more than one type. T. amylovorum, T. medium, T.socranskii, and T. vincentii were detected in 72%, 87%, 76 % , and 28% respectively with higher percentage at pocket depths >5 mm. Molecular method was able to detect Treponemal species even after scaling, however, in lower percentage than before it.
1 citations
Cites background from "Microbiological diagnosis of the se..."
...provetella intermedia, Actinobacillus actinomycecomitance, Porpheromonas gingivalis, Tanerella forsythus, Treponema denticola etc.(Boyanova et al., 2009), detection and identification of large numbers of anaerobes implicated in oral polymicrobial infections are hampered by the complexity of oral biofilm ( Bayingana et al ....
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...…evaluated e.g. provetella intermedia, Actinobacillus actinomycecomitance, Porpheromonas gingivalis, Tanerella forsythus, Treponema denticola etc.(Boyanova et al., 2009), detection and identification of large numbers of anaerobes implicated in oral polymicrobial infections are hampered by the…...
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01 Jan 2017
TL;DR: Fek farmakologis ekstrak kulit jeruk nipis ............................ 18 2.4 .1.1 Minyak atsiri .............................................................. 18 Universitas Sumatera Utara.
Abstract: ......................................................................................................... ii KATA PENGANTAR.......................................................................................... iii RIWAYAT HIDUP.............................................................................................. vi DAFTAR ISI......................................................................................................... vii DAFTAR TABEL............................................................................................. x DAFTAR GAMBAR........................................................................................ xii DAFTAR LAMPIRAN..................................................................................... xiii BAB 1 PENDAHULUAN 1.1 Latar Belakang............................................................................... 1 1.2 Rumusan Masalah.......................................................................... 3 1.3 Tujuan Penelitian........................................................................... 3 1.4 Hipotesa Penelitian........................................................................ 4 1.5 Manfaat Penelitian......................................................................... 4 BAB 2 TINJAUAN PUSTAKA 2.1 Aggregatibacter actinomycetemcomitans..................................... 8 2.2 Porphyromonas gingivalis............................................................ 12 2.3 Fusobacterium nucleatum ........................................................... 15 2.4 Jeruk Nipis ................................................................................. 16 2.4 .1. Efek farmakologis ekstrak kulit jeruk nipis ..................... 18 2.4.1.1 Minyak atsiri .............................................................. 18 Universitas Sumatera Utara
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...The International Journal of Biotechnology, 2013, 2(6):113-22....
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References
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TL;DR: In this article, the purpose of the present investigation was to identify potential periodontal pathogens among newly identified species and phylotypes, and species-specific ribosomal 16S primers for PCR amplification were developed for detection of new species.
Abstract: Recent investigations of the human subgingival oral flora based on ribosomal 16S cloning and sequencing have shown many of the bacterial species present to be novel species or phylotypes. The purpose of the present investigation was to identify potential periodontal pathogens among these newly identified species and phylotypes. Species-specific ribosomal 16S primers for PCR amplification were developed for detection of new species. Associations with chronic periodontitis were observed for several new species or phylotypes, including uncultivated clones D084 and BH017 from the Deferribacteres phylum, AU126 from the Bacteroidetes phylum, Megasphaera clone BB166, clone X112 from the OP11 phylum, and clone I025 from the TM7 phylum, and the named species Eubacterium saphenum, Porphyromonas endodontalis, Prevotella denticola, and Cryptobacterium curtum. Species or phylotypes more prevalent in periodontal health included two uncultivated phylotypes, clone W090 from the Deferribacteres phylum and clone BU063 from the Bacteroidetes, and named species Atopobium rimae and Atopobium parvulum.
489 citations
TL;DR: Chronic periodontitis is the result of a global perturbation of the oral bacterial ecology rather than a disease-site specific microbial shift, and more differences were found in the bacterial profile between subjects withperiodontitis and healthy subjects than between deep and shallow sites within the same subject.
Abstract: Most studies of the bacterial etiology of periodontitis have used either culture-based or targeted DNA approaches, and so it is likely that pathogens remain undiscovered. The purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. Samples from subjects with periodontitis and controls were analyzed using ribosomal 16S cloning and sequencing. Several genera, many of them uncultivated, were associated with periodontitis, the most numerous of which were gram positive, including Peptostreptococcus and Filifactor. The genera Megasphaera and Desulfobulbus were elevated in periodontitis, and the levels of several species or phylotypes of Campylobacter, Selenomonas, Deferribacteres, Dialister, Catonella, Tannerella, Streptococcus, Atopobium, Eubacterium, and Treponema were elevated in disease. Streptococcus and Veillonella spp. were found in high numbers in all samples and accounted for a significantly greater fraction of the microbial community in healthy subjects than in those with periodontitis. The microbial profile of periodontal health also included the less-abundant genera Campylobacter, Abiotrophia, Gemella, Capnocytophaga, and Neisseria. These newly identified candidates outnumbered Porphyromonas gingivalis and other species previously implicated as periodontopathogens, and it is not clear if newly identified and more numerous species may play a more important role in pathogenesis. Finally, more differences were found in the bacterial profile between subjects with periodontitis and healthy subjects than between deep and shallow sites within the same subject. This suggests that chronic periodontitis is the result of a global perturbation of the oral bacterial ecology rather than a disease-site specific microbial shift.
445 citations
TL;DR: The present findings using a large-scale analysis allowed the inclusion of some newly named species and as-yet-uncultivated phylotypes in the set of candidate pathogens associated with this disease.
Abstract: Samples from infected root canals of 43 teeth with chronic apical periodontitis were analyzed for the presence and relative levels of 83 oral bacterial species and/or phylotypes using a reverse-capture checkerboard hybridization assay. Associations between the most frequently detected taxa were also recorded. The most prevalent taxa were Olsenella uli (74%), Eikenella corrodens (63%), Porphyromonas endodontalis (56%), Peptostreptococcus anaerobius (54%), and Bacteroidetes oral clone X083 (51%). When prevalence was considered only for bacteria present at levels >10(5), Bacteroidetes clone X083 was the most frequently isolated bacterium (37%), followed by Parvimonas micra (28%), E. corrodens (23%), and Tannerella forsythia (19%). The number of target taxa per canal was directly proportional to the size of the apical periodontitis lesion, with lesions >10 mm in diameter harboring a mean number of approximately 20 taxa. Several positive associations for the most prevalent taxa were disclosed for the first time and may have important ecological and pathogenic implications. In addition to strengthening the association of several cultivable named species with chronic apical periodontitis, the present findings using a large-scale analysis allowed the inclusion of some newly named species and as-yet-uncultivated phylotypes in the set of candidate pathogens associated with this disease.
148 citations
TL;DR: Rapid detection and quantification of periodontal pathogens in mouthwash samples are possible by real-time PCR, which is significantly less time-consuming than subgingival sampling with paper points.
Abstract: Background: Saliva has been studied for the presence of subgingival pathogens in periodontitis patients. With the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this approach not suitable for practical use in the microbial diagnosis of periodontitis patients. The real-time polymerase chain reaction (PCR) technique represents a very sensitive technique to detect and quantify bacterial pathogens. The aim of the study was to compare the presence and numbers of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythensis, Prevotella intermedia, and Micromonas micros in subgingival plaque and mouthwash samples by the anaerobic culture and real-time PCR techniques. Methods: Pooled subgingival plaque samples and 10-ml mouthwash samples were collected from 21 adult patients with periodontitis and analyzed by quantitative anaerobic culture and real-time PCR for A. actinomycetemcomitans, P. gingivalis, T. forsythensis, P. intermedia, and M. micros. Results: The detection frequency of A. actinomycetemcomitans, P. gingivalis, and T. forsythensis in subgingival plaque was identical by culture and real-time PCR and was higher for P. intermedia and M. micros by real-time PCR. The highest detection frequencies for the target bacteria were found in mouthwash samples by real-time PCR. The additional value of the real-time PCR to detect target bacteria was 38% for P. gingivalis, 73% for T. forsythensis, 77% for P. intermedia, and 71% for M. micros. The sensitivity to detect target species in mouthwash by real-time PCR was 100% for all test species except for P. intermedia (93.8%). Conclusions: Rapid detection and quantification of periodontal pathogens in mouthwash samples are possible by real-time PCR. The procedure is significantly less time-consuming than subgingival sampling with paper points. This approach to detect major periodontal pathogens in mouthwash samples may simplify microbial diagnosis in periodontitis patients and may be used to monitor periodontal treatment.
125 citations
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...DISCUSSION Boutaga et al. (2007) have reported the real-time polymerase chain reaction (PCR) to be a very sensitive technique to detect bacterial periodontopathogens....
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TL;DR: It is demonstrated that a wide range of oral species may be detected on or in crevicular epithelial cells from sites with periodontitis and from periodontally healthy sulci.
Abstract: Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. This study determined the prevalence of a group of oral bacterial species on or in epithelial cells derived from periodontal pockets and the gingival crevice of subjects with periodontitis. Samples of epithelial cells were obtained from 120 sites with periodontal pockets > or =4 mm and 92 periodontally healthy sites from 49 patients (mean age 46.3+/-1.4 years; 43% males) with chronic periodontitis. Bacteria in or on epithelial cells were separated from unattached bacteria by Percoll density-gradient centrifugation. The presence and levels of 33 oral species were determined in epithelial cell samples by whole genomic DNA probes and the checkerboard method. The most frequently detected species were Porphyromonas gingivalis (42%), Treponema denticola (38%), Prevotella intermedia (37%), Streptococcus intermedius (36%), Campylobacter rectus (35%), Streptococcus sanguinis (35%) and Streptococcus oralis (34%). Species of Actinomyces were found in low prevalence and levels. The data indicated that there were more micro-organisms on or in epithelial cells obtained from periodontal pockets than from healthy sulci; however, no significant differences regarding the percentage and level of any specific species were found between these sites. Veillonella parvula, S. oralis, Streptococcus gordonii and Streptococcus mitis tended to be more prevalent in sites without disease. These findings demonstrated that a wide range of oral species may be detected on or in crevicular epithelial cells from sites with periodontitis and from periodontally healthy sulci.
100 citations