Microbiological Effects of Consuming a Synbiotic Containing Bifidobacterium bifidum, Bifidobacterium lactis, and Oligofructose in Elderly Persons, Determined by Real-Time Polymerase Chain Reaction and Counting of Viable Bacteria
Citations
192 citations
188 citations
182 citations
Cites background from "Microbiological Effects of Consumin..."
...The integrity of DNA was checked by agarose gel electrophoresis and UV-light photography with ethidium bromide staining....
[...]
...(4) Patients with decompensated HBV cirrhosis: HBsAg positive, HBeAg positive, and HBV DNA detectable with cirrhosis, based on liver biopsy or obvious findings on ultrasound imaging, but without GI bleeding....
[...]
...Although rRNA gene copies cannot be directly converted into cell numbers due to different numbers of rRNA operons in different species, plasmid DNA standards are often used because they can generate easily standards that can be independently quantified and converted to numbers of copies of target DNA [2, 3, 42]....
[...]
...(3) Patients with chronic hepatitis B: HBsAg positive, HBeAg positive, and HBV DNA detectable with abnormal liver function tests....
[...]
...The copy number of virulence genes per microliter of crude DNA template was determined by comparison with serially diluting plasmid DNA standards running on the same plate....
[...]
177 citations
174 citations
References
7,232 citations
"Microbiological Effects of Consumin..." refers background in this paper
...Inulin-type fructans are commonly used prebiotics, which have been defined as nondigestible dietary components that selectively stimulate the growth and/or activities of bacteria in the large bowel [15]....
[...]
4,055 citations
3,391 citations
1,051 citations
Additional excerpts
...The aim of this double-blind, randomized, controlled feeding trial was to study the effects of ingestion of a synbiotic containing 2 bifidobacterial species (Bifidobacterium bifidum BB-02 and Bifidobacterium lactis BL-01) and oligofructose on the composition of intestinal bifidobacteria and Lactobacillus populations in older people....
[...]
...Another reason why rRNA gene copy numbers were higher than cell numbers is that rRNA operons vary widely in bacteria, and between 2 to 5 rRNA operons have been found in different species belonging to the genus Bifidobacterium [34]....
[...]
...A randomized, double-blind, controlled feeding trial was done with 18 healthy elderly volunteers (age, 162 years) using a synbiotic comprising Bifidobacterium bifidum BB-02 and Bifidobacterium lactis BL-01 (probiotics) together with an inulin-based prebiotic (Synergy 1; Orafti)....
[...]
...With use of primers specific for the genus Bifidobacterium, significantly higher copy numbers of target DNA were found in the synbiotic group during the feeding period (weeks 4 and 5) and during the postfeeding period (weeks 6 and 8) (table 4)....
[...]
...Bifidobacterium adolescentis, Bifidobacterium angulatum, and Bi- Synbiotic Feeding Study • CID 2005:40 (1 January) • 33 fidobacterium dentium predominated (table 2); Bifidobacterium breve, Bifidobacterium longum, and Bifidobacterium pullorum were occasionally found; and Bifidobacterium boum and Bifidobacterium catenulatum were detected in only a few stool samples....
[...]
979 citations
"Microbiological Effects of Consumin..." refers background in this paper
...Bifidobacterium genus Bif164F 5′-GGG TGG TAA TGC CGG ATG-3′ 457 59 [22] Bif601R 5′-TAA GCC ATG GAC TTT CAC ACC-3′ ....
[...]