Microbiological Effects of Consuming a Synbiotic Containing Bifidobacterium bifidum, Bifidobacterium lactis, and Oligofructose in Elderly Persons, Determined by Real-Time Polymerase Chain Reaction and Counting of Viable Bacteria
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100 citations
Cites background from "Microbiological Effects of Consumin..."
...Also, certain synbiotics have been observed to increase levels of lactobacilli and bifidobacteria [24,28]....
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94 citations
Cites background from "Microbiological Effects of Consumin..."
...bifidum and total bifidobacteria and lactobacilli in the microbiota of elderly subjects [45]....
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87 citations
Cites result from "Microbiological Effects of Consumin..."
...Probiotic intervention resulted in changes in the composition of the Bifidobacterium microbiota composition—a result which is inline with earlier reports from studies with probiotics (Ouwehand et al. 2008a) and synbiotics (Bartosch et al. 2005; Ouwehand et al. 2008b)....
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85 citations
Cites background from "Microbiological Effects of Consumin..."
...Primers used to enumerate selected bacterial target groups by quantitative PCR Primers Sequence 50 –30 Target group Reference Eub338F 50-ACTCCTACGGGAGGCAGCAG-30 Total bacteria Guo et al.(48) Eub518R 50-ATTACCGCGGCTGCTGG-30 Bac303F 50-GAAGGTCCCCCACATTG-30 Bacteroides spp. Ramirez-Farias et al.(49) Bfr-Femrev 50-CGCKACTTGGCTGGTTCAG-30 Firm934F 50-GGAGYATGTGGTTTAATTCGAAGCA-30 Firmicutes Guo et al.(48) Firm1060R 50-AGCTGACGACAACCATGCAC-30 Clep866mF 50-TTAACACAATAAGTWATCCACCTGG-30 Clostridium Cluster IV Ramirez-Farias et al.(49) Clep1240mR 50-ACCTTCCTCCGTTTTGTCAAC-30 RrecF 50-GCGGTRCGGCAAGTCTGA-30 Roseburia spp./E. rectale Furet et al.(50) Rrec630mR 50-CCTCCGACACTCTAGTMCGAC-30 Fprau223F 50-GATGGCCTCGCGTCCGATTAG-30 Faecalibacterium prausnitzii Bartosch et al.(51) Fprau420R 50-CCGAAGACCTTCTTCCTCC-30 EhalF 50-GCGTAGGTGGCAGTGCAA-30 Eubacterium hallii Ramirez-Farias et al.(49) EhalR 50-GCACCGRAGCCTATACGG-30 dsrA_F336 50-CTGCGAATATGCCTGCTACA-30 SRB, dsrA gene Pereyra et al.(52) dsrA_R533 50-TGGTCGARCTTGATGTCGTC-30 F_Lacto 05 50-AGCAGTAGGGAATCTTCCA-30 Lactobacillus/Pediococcus/Leuconostoc spp. Furet et al.(50) R_Lacto 04 50-CGCCACTGGTGTTCYTCCATATA-30 xfp-fw 50-ATCTTCGGACCBGAYGAGAC-30 Bifidobacteria phosphoketolase Cleusix et al.(53) xfp-rv 50-CGATVACGTGVACGAAGGAC-30 Eco1457F 50-CATTGACGTTACCCGCAGAAGAAGC-30 Enterobacteriaceae Bartosch et al.(51) Eco1652R 50-CTCTACGAGACTCAAGCTTGC-30 SRB, sulphate-reducing bacteria; dsrA, dissimilatory sulphite-reductase subunit A....
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...xfp-rv 50-CGATVACGTGVACGAAGGAC-30 Eco1457F 50-CATTGACGTTACCCGCAGAAGAAGC-30 Enterobacteriaceae Bartosch et al.((51)) Eco1652R 50-CTCTACGAGACTCAAGCTTGC-30...
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...Rrec630mR 50-CCTCCGACACTCTAGTMCGAC-30 Fprau223F 50-GATGGCCTCGCGTCCGATTAG-30 Faecalibacterium prausnitzii Bartosch et al.((51)) Fprau420R 50-CCGAAGACCTTCTTCCTCC-30 EhalF 50-GCGTAGGTGGCAGTGCAA-30 Eubacterium hallii Ramirez-Farias et al....
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81 citations
Cites background from "Microbiological Effects of Consumin..."
...Another way to promote bifidobacteria population in the elderly may be probiotics (i.e. bifidobacteria supplementation) (Bartosch et al. 2005, Ahmed et al. 2007, Lahtinen et al. 2009) or symbiotics combining prebiotics and bifidobacteria (Bartosch et al. 2005, Macfarlane et al. 2008)....
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References
7,232 citations
"Microbiological Effects of Consumin..." refers background in this paper
...Inulin-type fructans are commonly used prebiotics, which have been defined as nondigestible dietary components that selectively stimulate the growth and/or activities of bacteria in the large bowel [15]....
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Additional excerpts
...The aim of this double-blind, randomized, controlled feeding trial was to study the effects of ingestion of a synbiotic containing 2 bifidobacterial species (Bifidobacterium bifidum BB-02 and Bifidobacterium lactis BL-01) and oligofructose on the composition of intestinal bifidobacteria and Lactobacillus populations in older people....
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...Another reason why rRNA gene copy numbers were higher than cell numbers is that rRNA operons vary widely in bacteria, and between 2 to 5 rRNA operons have been found in different species belonging to the genus Bifidobacterium [34]....
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...A randomized, double-blind, controlled feeding trial was done with 18 healthy elderly volunteers (age, 162 years) using a synbiotic comprising Bifidobacterium bifidum BB-02 and Bifidobacterium lactis BL-01 (probiotics) together with an inulin-based prebiotic (Synergy 1; Orafti)....
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...With use of primers specific for the genus Bifidobacterium, significantly higher copy numbers of target DNA were found in the synbiotic group during the feeding period (weeks 4 and 5) and during the postfeeding period (weeks 6 and 8) (table 4)....
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...Bifidobacterium adolescentis, Bifidobacterium angulatum, and Bi- Synbiotic Feeding Study • CID 2005:40 (1 January) • 33 fidobacterium dentium predominated (table 2); Bifidobacterium breve, Bifidobacterium longum, and Bifidobacterium pullorum were occasionally found; and Bifidobacterium boum and Bifidobacterium catenulatum were detected in only a few stool samples....
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979 citations
"Microbiological Effects of Consumin..." refers background in this paper
...Bifidobacterium genus Bif164F 5′-GGG TGG TAA TGC CGG ATG-3′ 457 59 [22] Bif601R 5′-TAA GCC ATG GAC TTT CAC ACC-3′ ....
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