Microdroplet Mass Spectrometry Enables Extremely Accelerated Pepsin Digestion of Proteins
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TLDR
In this article, an ultrarapid pepsin in-spray digestion of two different proteins, cytochrome c and RocC, at low pH values was investigated and carefully designed control experiments allowed to unambiguously prove that enzymatic protein cleavage almost exclusively occurs within the spray at a millisecond time scale and not prior to microdroplet generation.About:
This article is published in Journal of the American Society for Mass Spectrometry.The article was published on 2021-06-08 and is currently open access. It has received 4 citations till now. The article focuses on the topics: Protein digestion & Electrospray ionization.read more
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Investigation of Tryptic Protein Digestion in Microdroplets and in Bulk Solution.
TL;DR: This study suggests that microdroplet digestion, a fast digestion approach, could be adopted for bottom-up proteomics research and for peptide mapping of mAbs to characterize site-specific deamidation and glycosylation, for the purpose of development of biopharmaceuticals.
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Efficient Sample Preparation System for Multi-Omics Analysis via Single Cell Mass Spectrometry.
Peng Zhao,Yongxiang Feng,Junhan Wu,Junwen Zhu,Jinlei Yang,Xiaoxiao Ma,Zheng Ouyang,Xinrong Zhang,Wenyi Zhang,Wenhui Wang +9 more
TL;DR: Wang et al. as discussed by the authors developed a 10-pL-level microwell chip for housing individual single cells, whose proteins were found to be digested in 5 min, which is 144 times shorter than traditional bulk digestion.
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Rapid Characterization of Antibodies via Automated Flow Injection Coupled with Online Microdroplet Reactions and Native-pH Mass Spectrometry.
TL;DR: In this article , the authors integrated a microdroplet protein digestion technique with automated sample flow injection and online mass spectrometry (MS) analysis, to develop a rapid and robust method for structural characterization of monoclonal antibodies.
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Online Cross-Linking of Peptides and Proteins during Contained-Electrospray Ionization Mass Spectrometry.
TL;DR: In this article , a contained-electrospray (ES) experiment was described to integrate peptide/protein cross-linking with the general mass spectrometry (MS) workflow.
References
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Ambient Mass Spectrometry
TL;DR: In DESI as discussed by the authors, electrically charged droplets are directed at the ambient object of interest; they release ions from the surface, which are then vacuumed through the air into a conventional mass spectrometer.
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TL;DR: The new version of mMass is based on a stand-alone Python library, which provides the basic functionality for data processing and interpretation, and can serve as a good starting point for other developers in their projects.
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Organic Reactions in Microdroplets: Reaction Acceleration Revealed by Mass Spectrometry.
TL;DR: This Minireview introduces droplet and thin-film acceleration phenomena and summarizes recent methods applied to study accelerated reactions in confined-volume, high-surface-area solutions.
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Accelerated bimolecular reactions in microdroplets studied by desorption electrospray ionization mass spectrometry
TL;DR: In this article, it is shown that the environment in the microdroplet differs from that of the bulk: (i) the pH of the solution moves towards the extremes, (ii) the concentrations of the reagents increase, (iii) the relative surface area increases and collision frequencies increase.
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Electrosonic Spray Ionization. A Gentle Technique for Generating Folded Proteins and Protein Complexes in the Gas Phase and for Studying Ion−Molecule Reactions at Atmospheric Pressure
TL;DR: Evidence is provided that the method is capable of generating fully desolvated protein ions at atmospheric pressure and allows the technique to be used for the study of ion-molecule reactions at atmospheric Pressure.