Microfabricated multilayer parylene‐C stencils for the generation of patterned dynamic co‐cultures
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...In a proof-of-principle experiment, mouse ES cells were sequentially micropatterned with fibroblasts and hepatocytes (69)....
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Cites background or methods from "Microfabricated multilayer parylene..."
...Multilayer parylene stencils [50,51] and ultra-thick reusable parylene stencils [52] have been also developed....
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...For example, the total number of different cell types that could be sequentially patterned as co-cultures was increased from three to five using a three-layers parylene stencils compared to just a single layer stencil [51]....
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"Microfabricated multilayer parylene..." refers background or methods in this paper
...The in vitro deposition of ECM and the subsequent assembly of cells with control over cell position(14) and spatial organization(15) are of great importance in tissue engineering.(1) Previously, patterned co-cultures of two or more cell types have been generated using approaches such as photolithography, layer-by-layer deposition of cell-adhesive materials,(12,20) elastomeric membranes, andmicrofluidics....
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...The signals in the cellular microenvironment comprise of physicochemical factors, mechanical signals as well as cell–cell, cell-soluble factor, and cell– extracellular matrix (ECM) interactions.(1) Specifically, direct or indirect cell–cell interactions have been implicated in regulating a variety of cellular responses....
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