Journal ArticleDOI
Micropropagation of Centella asiatica (L.), a valuable medicinal herb
Reads0
Chats0
TLDR
Although bud break was dependent on BA supply, the synergistic combination of 22.2 μM BA and 2.68 μM NAA induced the optimum frequency of shoot formation as well as shoot number of Centella asiatica plants.Abstract:
A protocol is described for rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Centella asiatica (L) by enhanced axillary bud proliferation in nodal segments isolated from mature plants Although bud break was dependent on BA supply, the synergistic combination of 222 μM BA and 268 μM NAA induced the optimum frequency (91%) of shoot formation as well as shoot number (4 to 5 shoots per node) Subculturing of nodal segments harvested from the in vitro derived axenic shoots on the multiplication medium enabled continuous production of healthy shoots with similar frequency MS medium supplemented with 67 μM BA and 288 μM IAA was found most suitable for shoot elongation Rooting was highest (90%) on full-strength MS medium containing 246 μM IBA Micropropagated plants established in garden soil were uniform and identical to the donor plant with respect to growth characteristics This micropropagation procedure could be useful for raising a stock of genetically homogenous plant material for field cultivationread more
Citations
More filters
Journal ArticleDOI
Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) Urban elicited by methyl jasmonate
Ok-Tae Kim,Kyong-Hwan Bang,Yu-Su Shin,Min-Jeong Lee,Su-Jin Jung,Dong-Yun Hyun,Young-Chang Kim,Nak-Sul Seong,Seon-Woo Cha,Baik Hwang +9 more
TL;DR: Transformed root (“hairy root”) cultures have been shown to be a good model for the study of many secondary metabolites and production of C. asiatica hairy roots could be optimized and the resulting cultures could be elicited with MJ treatment for enhanced production of as iaticoside.
Journal ArticleDOI
Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) urban by elicitors
TL;DR: Although asiaticoside content did not change when TDZ was added to medium containing an elicitor, TDZ did increase shoot growth of C. as iatica and the interactive roles of MJ and TDZ are discussed in secondary metabolic production and biomass in whole plants.
Centella asiatica (l.): a plant with immense medicinal potential but threatened
TL;DR: Centella asiatica (L.) is a perennial, creeper, faintly aromatic and a valuable medicinal herb of both Old World and the New World as discussed by the authors, it is distributed throughout tropical and subtropical regions of World such as India, China, Nepal, Madagascar, Srilanka and Indonesia etc.
Journal ArticleDOI
Comparative analysis of active constituents in Centella asiatica samples from Madagascar: Application for ex situ conservation and clonal propagation
Denis Randriamampionona,Billo Diallo,Francisco Rakotoniriana,Christian Rabemanantsoa,Kiban Cheuk,Anne-Marie Corbisier,Jacques Mahillon,Suzanne Ratsima-Manga,Mondher El Jaziri +8 more
TL;DR: Acetoxycentellynol, a C(15)-polyacetylene, was found to be accumulated up to 18 times more in in vitro plants as compared to plant material collected in situ, and in vitro propagation of C. asiatica was successfully achieved in hormone-free medium.
Journal ArticleDOI
Medicinal plants: An invaluable, dwindling resource in sub-Saharan Africa.
TL;DR: The rich flora occurring in sub-Saharan Africa suggests enormous potential for discovery of new chemical entity with therapeutic value, however, concerted efforts focused on documenting the conservation status of African medicinal plants are pertinent.
References
More filters
Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Book
Statistical Procedures for Agricultural Research
TL;DR: This paper presents the results of a series of experiments conducted in farmers' fields in the Czech Republic over a period of three years to investigate the effects of agricultural pesticides on animal welfare and human health.
Journal ArticleDOI
Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation.
Y. Sahoo,Pradeep K. Chand +1 more
TL;DR: Although callus-free multiple-shoot formation was a function of cytokinin activity alone, faster bud break coupled with an enhanced frequency of shoot development and internode elongation were dependent on the synergistic influence of gibberellic acid (GA3) along with BA when used at an optimal concentration.
Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more