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Journal Article•DOI•

Micropropagation of Hemidesmus indicus for cultivation and production of 2-hydroxy 4-methoxy benzaldehyde

S. Sreekumar, S. Seeni, P. Pushpangadan
01 Sep 2000-Plant Cell Tissue and Organ Culture (Kluwer Academic Publishers)-Vol. 62, Iss: 3, pp 211-218
TL;DR: After 12 months of cultivation in the field, the micropropagated plants were stable and showed uniform morphological and growth characteristic and the concentration of the root specific compound, 2-hydroxy 4-methoxy benzaldehyde per plant was 2–3 fold higher in micropropAGated plants though on unit dry root biomass basis it remained the same between two sources of plants.
Abstract: Caulogenic responses of various explant types from 12-month-old plants of Hemidesmus indicus were tested Second and third visible nodes (05 cm) from the apex and root segments (05 cm) were the most and least regenerative respectively, with the formation of 937 and 26 shoots in 4 weeks on half strength MS medium supplemented with 222 μM BA and 107 μM NAA and 444 μM BA and 269 μM NAA respectively Caulogenic ability of the nodes decreased with increasing maturity Shoot buds initiated upon the young nodes on day 10 developed into 72 cm long shoots within 4 weeks thereby making a shoot elongation phase unnecessary Nodal explants of the in vitro raised shoots subcultured in the same medium produced 932 shoots of 71 cm length in 3–4 weeks, similar to those of the mature plant-derived nodes Multiplication through subculture of the nodes up to 25 passages of 4 weeks each was achieved without decline Shoot cultures were rooted in quarter salt strength MS medium containing 98 μM IBA and the rooted plants were hardened for establishment in pots at 96% rate Four months after establishment, the micropropagated plants were stable and showed uniform morphological and growth characteristic After 12 months of cultivation in the field, on an average micropropagated plant consisted of 4–5 shoots, 5–8 branches per shoot and increased root biomass (135 g) compared to the poor growth (single shoot and 2–3 branches) and root production (46 g) values obtained with plants raised from conventional rooted stem cuttings The concentration of the root specific compound, 2-hydroxy 4-methoxy benzaldehyde per plant was 2–3 fold higher in micropropagated plants though on unit dry root biomass (012% per g dry wt) basis it remained the same between two sources of plants
Citations
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Journal Article•DOI•
Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis

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K. P. Martin1•
University of Calicut1
01 Aug 2002-Plant Cell Reports
TL;DR: Efficient protocols of axillary bud multiplication and indirect organogenesis were established for Holostemma ada-kodien Schult and half-strength solid MS or liquid medium with 0.05 mg l–1 IBA exhibited the best in vitro rooting.
Abstract: Efficient protocols of axillary bud multiplication and indirect organogenesis were established for Holostemma ada-kodien Schult. (Asclepiadaceae). Murashige and Skoog (MS) medium supplemented with 2.0 mg l–1 N6-benzylaminopurine (BAP) and 0.5 mg l–1 indole-3-butyric acid (IBA) induced an average of eight shoots per node and was the best for axillary bud proliferation. Subsequent cultures enhanced the number of shoots. The explant source of callus and the growth regulator inducing the callus exhibited significant influence on organogenesis. Callus developed from the basal cut end of the node explants differentiated more than 15 shoots on MS medium fortified with 1.5 mg l–1BAP. Callus from internode explants developed fewer shoots than callus from the basal cut ends of node explants. Leaf-derived callus did not undergo organogenesis. The abscission of leaves and shoot tips of the developed shoots was prevented by the addition of AgNO3 or CoCl2, but with a concomitant significant reduction in the number of shoots. Half-strength solid MS or liquid medium with 0.05 mg l–1 IBA exhibited the best in vitro rooting. Ninety percent of the rooted shoots survived in the field.

191 citations

Journal Article•DOI•
Rapid in vitro propagation of medicinally important Ceropegia candelabrum

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M.R. Beena1, K. P. Martin2, Pulugurtha Bharadwaja Kirti1, Molly Hariharan2•
University of Hyderabad1, University of Calicut2
01 Mar 2003-Plant Cell Tissue and Organ Culture
TL;DR: Protocol for rapid in vitro propagation of Ceropegia candelabrum L. (Asclepiadaceae) through axillary bud multiplication was established and plantlets successfully established in field exhibited morphological characters identical to mother plants.
Abstract: Protocol for rapid in vitro propagation of Ceropegia candelabrum L. (Asclepiadaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 8.87 μM N 6-benzyladenine (BA) and 2.46 μM indole-3-butyric acid (IBA) was best suited for axillary bud proliferation inducing a mean of eight shoots / node. Excision and culture of the nodal segments from the in vitro shoots on fresh medium with same concentrations of BA and IBA facilitated development of more than 10 shoots / node. Subsequent cultures enhanced the rate of shoot proliferation. Shoots developed were rooted best on half strength MS with 0.49 μM IBA. Starting from a single node explant, 250 rooted shoots were obtained within 120 days. Plantlets established in pots exhibited 90% survival. Plantlets successfully established in field exhibited morphological characters identical to mother plants.

109 citations

Cites background or methods from "Micropropagation of Hemidesmus indi..."

  • ...Ten cultures Hemidesmus indicus (Sreekumar et al., 2000), and were raised for each treatment and all experiments Holostemma ada-kodien (Martin, 2002)....

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  • ...61: 97–105(Komalavalli and Rao, 2000), Hemidesmus indicus Martin KP (2002) Rapid propagation of Holostemma ada-kodien (Sreekumar et al., 2000), and Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplica(Martin, 2002). tion and indirect organogenesis....

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  • ...BA was the ture on Asclepiadacean medicinal plants like Gymnema sylvestre (Komalavalli and Rao, 2000), rectly to small pots containing sterile (soilrite1sand at Hemidesmus indicus (Sreekumar et al., 2000) and 1:1) revived growth within 15 days....

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Journal Article•DOI•
An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant

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Mohd Faisal1, Naseem Ahmad1, Mohammad Anis1•
Aligarh Muslim University1
03 Aug 2007-Plant Biotechnology Reports
TL;DR: The described method can be successfully employed for large-scale multiplication and long-term in vitro conservation of T. indica and shows any immediate detectable phenotypic variation.
Abstract: An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Tylophora indica (Burm. f.) Merrill, via enhanced axillary bud proliferation from nodal explants collected from young shoots of a two-year-old plant. The physiological effects of growth regulators [6-benzyladenine (BA), kinetin (Kin) thidiazuron (TDZ), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA)], ascorbic acid (AA), different strengths of Murashige and Skoog (MS) medium and various pH levels on in vitro morphogenesis were investigated. The highest number (8.6 ± 0.71) of shoots and the maximum average shoot length (5.2 ± 0.31 cm) were recorded on MS medium supplemented with 2.5 μM BA, 0.5 μM NAA and 100 mg/l AA at pH 5.8. Rooting was best achieved on half-strength MS medium augmented with 0.5 μM IBA. The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing garden soil and grown in a greenhouse with a 90% survival rate. The regenerated plants did not show any immediate detectable phenotypic variation. The described method can be successfully employed for large-scale multiplication and long-term in vitro conservation of T. indica.

96 citations

Cites background from "Micropropagation of Hemidesmus indi..."

  • ...…plants from the Asclepiadaceae family, such as Gymnema sylvestre (Reddy et al. 1998), Holostemma annulare (Sudha et al. 1998), Hemidesmus indicus (Sreekumar et al. 2000), Holostemma ada-kodien (Martin 2002), Leptadenia reticulata (Arya et al. 2003), and Ceropegia candelabrum (Beena et al. 2003)....

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  • ...formosa (Jusaitis 1997), Hemidesmus indicus (Sreekumar et al. 2000), Cunila galoides (Fracaro and Echeverrigaray 2001), Holostemma ada-kodien (Martin 2002), Ceropegia candelabrum (Beena et al....

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  • ...The success of IBA in promoting efficient root induction has been reported for Swaisona formosa (Jusaitis 1997), Hemidesmus indicus (Sreekumar et al. 2000), Cunila galoides (Fracaro and Echeverrigaray 2001), Holostemma ada-kodien (Martin 2002), Ceropegia candelabrum (Beena et al. 2003), and Mucuna…...

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  • ...1998), Hemidesmus indicus (Sreekumar et al. 2000), Holostemma ada-kodien (Martin 2002), Leptadenia reticulata (Arya et al....

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Journal Article•DOI•
Influence of plant growth regulators on shoot proliferation and secondary metabolite production in micropropagated Huernia hystrix

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Stephen O. Amoo1, Johannes Van Staden1•
University of KwaZulu-Natal1
01 Feb 2013-Plant Cell Tissue and Organ Culture
TL;DR: Meta-topolin (mT) was more effective in improving shoot proliferation and phenolic production compared to BA and supplementation with low NAA concentrations resulted in reduced in vitro flavonoid production in most cases, when compared to treatments with cytokinin only.
Abstract: Although the effectiveness of topolins in plant tissue culture systems has recently been highlighted, there is a dearth of information on their interactions with auxins in relation to shoot organogenesis and secondary metabolite production. The current study evaluated the role of topolins singly or in combination with an auxin in comparison to 6-benzyladenine (BA) on shoot proliferation and secondary metabolite production of Huernia hystrix, a medicinal and ornamental stem-succulent of the endemic flora of southern Africa. Meta-topolin (mT) was more effective in improving shoot proliferation and phenolic production compared to BA. In general, the exogenous addition of α-naphthalene acetic acid (NAA) significantly increased shoot proliferation. The highest number of regenerated shoots (12.2 ± 0.98 shoots per explant) was recorded with medium containing 20 μM mT supplemented with 10 μM NAA and was three-times higher when compared to the treatments with cytokinin only. This suggests a synergistic interaction of auxin with cytokinin. On the other hand, supplementation with low NAA concentrations resulted in reduced in vitro flavonoid production in most cases, when compared to treatments with cytokinin only. Moreover, differences in cytokinin concentrations (even when used in combination with NAA equimolar concentrations) significantly affected secondary metabolite production in some cases. The current findings highlighted the differential effects of auxin-cytokinin interactions on shoot proliferation and the production of secondary metabolites in H. hystrix.

72 citations

Journal Article•DOI•
Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology

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P. N. Krishnan, Sabarimuthan William Decruse, R. K. Radha
01 Feb 2011-In Vitro Cellular & Developmental Biology – Plant
TL;DR: Propagating biotechnology tools in plant conservation program is a prerequisite to succeed in sustainable use and to complement the existing ex situ measures, and storage of these valuable genetic resources is equally important.
Abstract: Climate change, alien species, and use of land for intensive farming and development are causing severe threat to the plant genetic diversity worldwide. Hence, conservation of biodiversity is considered fundamental and also provides the livelihoods to millions of people worldwide. Medicinal plants play a key role in the treatment of a number of diseases, and they are only the source of medicine for majority of people in the developing world. The tropical regions of the world supply the bulk of current global demand for “natural medicine,” albeit with increasing threat to populations in the world and its genetic diversity. India is a major center of origin and diversity of crop and medicinal plants. India poses out 20,000 species of higher plants, one third of it being endemic and 500 species are categorized to have medicinal value. The Western Ghats is one of the major repositories of medicinal plants. It harbors around 4,000 species of higher plants of which 450 species are threatened. Currently, the number of species added to the red list category in this region is increasing, and the valuable genetic resources are being lost at a rapid rate. Demand for medicinal plants is increasing, and this leads to unscrupulous collection from the wild and adulteration of supplies. Providing high-quality planting material for sustainable use and thereby saving the genetic diversity of plants in the wild is important. During the last 25 years of intensive research, Tropical Botanic Garden and Research Institute has developed in vitro protocol for rapid regeneration and establishment of about 40 medicinally important rare and threatened plants of Western Ghats. In situ conservation alone would not be effective in safeguarding these important species. Thus, utilizing the biotechnoligical approach to complement ex situ conservation program is becoming vital. Propagating biotechnology tools in plant conservation program is a prerequisite to succeed in sustainable use and to complement the existing ex situ measures. In addition to propagation, storage of these valuable genetic resources is equally important. In vitro slow growth of 35 species and cryopreservation using embryo/meristem/seed in 20 different species of rare medicinal plants of this region is accomplished. Plants developed in vitro of ten medicinal plants, which have restricted distribution, were reintroduced in the natural habitat as well.

60 citations

Cites background from "Micropropagation of Hemidesmus indi..."

  • ...In vitro regeneration protocol standardized in medicinal plants of Western Ghats Species Regeneration pathway Explant Reference Acorus calamus Organogenesis Rhizome buds; Apical shoot Anu et al. 2001; Hettiarachchi et al. 1997 Adhatoda beddomeia Organogenesis Nodes Sudha and Seeni 1994 Aegle marmelosa Organogenesis Nodes; In vitro nodes Islam and Karim 1994, Ajithkumar and Seeni 1998 Aloe veera Organogenesis Nodes Singh and Sood 2009 Aristolochia indica Organogenesis Node, shoot tip Soniya and Sujitha 2006 Aristolochia tagala Organogenesis Node Animesh et al. 2007 Asparagus recemosus Organogenesis Nodes Nishritha and Sanjay 2008 Baliospermum montanum Organogenesis Nodes Sasikumar et al. 2009 Blepharestemma membranifolia a. Organogenesis Nodes Laksmi and Seeni 2001a Boesenbergia pulcherrimaa Organogenesis Shoot tips Anish et al. 2008 Calophyllum apetaluma Organogenesis Nodes Lakshmi and Seeni, 2003 Celastrus paniculatusa Organogenesis Nodes Laksmi and Seeni 2001b; Gerald et al. 2006 Centella asiatica Organogenesis Node Nath et al. 2000; Karthikeyan et al. 2009 Ceropegia sahyadrica Organogenesis Nodes Nikam and Savant 2007 Coleus forskohlii Organogenesis Stem tip Neelam et al. 1991; Rajasri and Sabita 2001 Costus speciosus Organogenesis Rhizome sections Malabadi et al. 2005 Curculigo orchioides Organogenesis Shoot bud Neelam et al. 2007 Curcuma amada Organogenesis Rhizome Prakash et al. 2004 Curcuma harithaa Organogenesis Rhizome tips Bejoy et al. 2006 Curcuma longa Organogenesis Rhizome buds Prathanturarug et al. 2005 Curcuma zedoaria Organogenesis Rhizome bud Loc et al. 2005, Stanly and Keng 2007 Datura metel Organogenesis Nodes Muthukumar et al. 2004 Decalepis arayalpathraa Organogenesis Node; Cotyledonary nodes Gangaprasad et al. 2005; Sudha et al. 2005 Decalepis hamiltonii Organogenesis Shoot tips Giridhar et al. 2005 Dioscorea bulbifera Organogenesis Node Asha and Nair 2007 Dioscorea pentaphylla Organogenesis leaf Asha and Nair 2005 Embelia ribes Organogenesis leaf Raghu et al. 2006b Geophila reniformis Organogenesis leaves Nisha et al. 2004 Gloriosa superba Organogenesis Apical bud Samarajeewa et al. 1993, Sayeed Hassan and Roy 2005 Hemidesmus indicusa Organogenesis; Somatic embryos Nodes; Sreekumar et al. 2000;Sarasan et al. 1994 Holarrhena pubescens Organogenesis Nodes, shoot tips Gerald et al. 2005 Holostemma annularea Organogenesis Nodes, In vitro root segments Sudha et al. 1998, Sudha et al. 2000 Holostemma ada-kodien Organogenesis Node Martin 2002 Hypericum mysorense Organogenesis Node Shilpashree and Ravishankar 2009 Kaempferia galanga Organogenesis Rhizome bud Swapna et al. 2004; Chithra et al. 2005 Kaempferia galangaaa Somatic embryos In vitro leaf Preetha et al. 2008 Kaempferia rotunda Organogenesis Rhizome bud Anand et al. 1997 Mahonia leschenaultiaa Organogenesis Nodes Radha and Seeni 2010c Morinda umbellataa Organogenesis node Lakshmi and Seeni 2002 Myristica malabarica Somatic embryos (Direct) Zygotic embryos Indira Iyer et al. 2009 Nothapodytes foetidaa Organogenesis Axenic seedling nodes Satheesh Kumar and Seeni 2000 Ophiorrhiza mungoa Organogenesis Axenic seedling shoots; In vitro nodes Benoy and Satheeshkumar 2004 Ophiorrhiza prostrata Organogenesis Leaf, internode Shahanaz et al. 2007 Oroxylum indicum Organogenesis Apical and axillary buds Gokhale and Bansal 2009 Piper longum Organogenesis Shoot tips Soniya and Das, 2002 recommended is cloning a sufficient number of propagules collected from source populations to copy maximum genetic diversity (McGlaughlin et al 2002)....

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  • ...Pilot-scale field cultivation trials of H. annulare (unpublished), Hemidesmus indicus (Sreekumar et al. 2000) and P.rosea (Satheesh Kumar and Seeni 2003) conducted at TBGRI proved early tuberization and increased number/ biomass of tubers in tissue-cultured plants....

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  • ...…superba Organogenesis Apical bud Samarajeewa et al. 1993, Sayeed Hassan and Roy 2005 Hemidesmus indicusa Organogenesis; Somatic embryos Nodes; Sreekumar et al. 2000;Sarasan et al. 1994 Holarrhena pubescens Organogenesis Nodes, shoot tips Gerald et al. 2005 Holostemma annularea Organogenesis…...

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  • ...(continued) Species Regeneration pathway Explant Reference Plumbago rosea a Organogenesis Nodes Benoy et al. 2007 Pterocarpus marsupium Organogenesis Seedling nodes Sharad et al. 2004 Rauvolfia micrantha a Organogenesis Nodes; In vitro nodes Sudha and Seeni 1996 Rauvolfia serpentina Organogenesis Shoot tips, nodes Sarker et al. 1996, Baksha et al. 2007 Rotula aquatica Organogenesis Node Delse et al. 2002; Chithra et al. 2004 Sarcostemma brevistigma Organogenesis Nodes Dennis and Surabhi 2009 Tinospora cordifolia Organogenesis Nodes Raghu et al. 2006a Trichopus zeylanicus a Organogenesis Shoot tips of axenic seedlings Krishnan et al. 1995 Tylophora indica Organogenesis Nodes Faisa et al. 2007 Utlaria salicifolia a Organogenesis Nodes Gangaprasad et al. 2003 Vitex negundo Organogenesis Nodes Noman et al. 2008 Zingiber officinalis Somatic embryos through callus In vitro Areal stem Lincy et al. 2009 Zingiber zerumbet Organogenesis Rhizome bud Stanly and Keng 2007 a Protocol for micropropagation have been developed at TBGRI suggest that rooted plants survive longer (up to 28mo at 25°C) than shoot clumps in culture bottles with polypropylene caps as enclosures....

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References
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Journal Article•DOI•
A revised medium for rapid growth and bio assays with tobacco tissue cultures

[...]

Toshio Murashige1, Folke Skoog1•
University of Wisconsin-Madison1
01 Jul 1962-Physiologia Plantarum
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Abstract: In experiments with tobacco tissue cultured on White's modified medium (basal meditmi hi Tnhles 1 and 2) supplemenk'd with kiticthi and hidoleacctic acid, a slrikin^' fourlo (ive-told intTease iu yield was ohtaitu-d within a three to Tour week j^rowth period on addition of an aqtteotis exlrarl of tobacco leaves (Fi^'ures 1 and 2). Subse(iueutly it was found Ihiit this jnoniotiou oi' f^rowih was due mainly though nol entirely to inorj^auic rather than organic con.stitttenls in the extract. In the isolation of Rrowth factors from plant tissues and other sources inorj '̂anic salts are fre(|uently carried along with fhe organic fraclioits. When tissue cultures are used for bioassays, therefore, il is necessary lo lake into account increases in growth which may result from nutrient elements or other known constituents of the medium which may he present in the te.st materials. To minimize interference trom rontaminaitis of this type, an altempt has heen made to de\\eh)p a nieditmi with such adequate supplies of all re(iuired tnineral nutrients and cotntnott orgattic cottslitueitls that no apprecial»le change in growth rate or yield will result from the inlroduclion of additional amounts in the range ordinarily expected to be present in tnaterials to be assayed. As a point of referetice for this work some of the culture media in mc)st common current use will he cotisidered briefly. For ease of comparis4)n Iheir mineral compositions are listed in Tables 1 and 2. White's nutrient .solution, designed originally for excised root cultures, was based on Uspeuski and Uspetiskaia's medium for algae and Trelease and Trelease's micronutrieni solution. This medium also was employed successfully in the original cttltivation of callus from the tobacco Iiybrid Nicotiana gtauca x A', tanijadorffii, atitl as further modified by White in 194̂ ^ and by others it has been used for the

63,098 citations

"Micropropagation of Hemidesmus indi..." refers methods in this paper

  • ...Shoot tip, node, internode (∼ 0.5 cm long), leaf (0.5 cm2 ) and root (∼ 1.0 cm) segments were dissected out and inoculated on to quarter, half and full strength MS (Murashige and Skoog, 1962) medium supplemented with 3% (w/v) sucrose, 0.6% (w/v) agar and growth regulators (µM)....

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A revised medium for the growth and bioassay with tobacco tissue culture

[...]

T Murashige, F Skoog
01 Jan 1962

16,251 citations

"Micropropagation of Hemidesmus indi..." refers methods in this paper

  • ...Shoot tip, node, internode (∼ 0.5 cm long), leaf (0.5 cm2 ) and root (∼ 1.0 cm) segments were dissected out and inoculated on to quarter, half and full strength MS (Murashige and Skoog, 1962) medium supplemented with 3% (w/v) sucrose, 0.6% (w/v) agar and growth regulators (µM)....

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  • ...0 cm) segments were dissected out and inoculated on to quarter, half and full strength MS (Murashige and Skoog, 1962) medium supplemented with 3% (w/v) sucrose, 0....

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Book•
Indian Medicinal Plants

[...]

Kanhoba Ranchoddas Kirtikar, Baman Das Basu
09 Jan 2018
TL;DR: Indian medicinal plants/, Indian medicinal plants /, مرکز فناوری اطلاعات و اصاع رسانی, کδاوρزی
Abstract: Indian medicinal plants / , Indian medicinal plants / , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

8,252 citations

Book•
Glossary of Indian Medicinal Plants

[...]

R. N. Chopra, S. L. Nayar, I. C. Chopra
01 Jan 1956

5,524 citations

"Micropropagation of Hemidesmus indi..." refers background in this paper

  • ...The root extract of this plant has been widely used: (1) in the traditional systems of medicine in India such as Ayurveda, Siddha and Unani mainly as blood purifier, diuretic anti-rheumatic and anti-diarrhoeal and anti-viper venom activity (Chopra et al., 1980; Alam et al., 1996)....

    [...]

Book•
Plant Propagation by Tissue Culture

[...]

Edwin F. George
01 Jan 1984
TL;DR: The Anatomy and Morphology of Tissue Cultured Plants M.V. Moshkov, G. V. Novikova, M. Stasolla, E. Zazimalova and E.F. George reveal the secrets of successful tissue culture and the challenges faced in implementing and sustaining such a system.
Abstract: Biographical Notes on Contributors.- 1.Plant Tissue Culture Procedure - Background E.F. George.- 2.Micropropagation: Uses and Methods E.F. George and P.C. Debergh.- 3.The Components Of Plant Tissue Culture Media I : Macro- and Micronutrients E.F. George and G-J. de Klerk.- 4.The Components Of Plant Tissue Culture Media II : Organic supplements, Organic Acids, Osmotic and pH Effects and Support systems T. Thorpe, C. Stasolla, E.C. Yeung, G-J. de Klerk, A. Roberts and E.F. George.- 5.Plant Growth Regulators: Introduction I: Auxins, Their Analogues And Inhibitors I. Machakova, E. Zazimalova and E.F. George.- 6.Plant Growth Regulators II: Cytokinins, Their Analogues And Antagonists J. van Staden, E. Zazimalova and E.F. George.- 7.Plant Growth Regulators III: Gibberellins, Ethylene, Abscisic Acid, Their Analogues And Inhibitors Miscellaneous Compounds I.E. Moshkov, G.V. Novikova, M.A. Hall and E.F. George.- 8.Developmental Biology D. Chriqui.- 9.Somatic Embryogenesis S. Von Arnold.- 10.Adventitious Regeneration P.B. Gahan and E.F. George.- 11.Stock Plant Physiological Factors Affecting Growth and Morphogenesis J. Preece.- 12.Effects Of The Physical Environment E.F. George and W. Davies.- 13.The Anatomy And Morphology Of Tissue Cultured Plants M. Ziv and J. Chen

2,358 citations

"Micropropagation of Hemidesmus indi..." refers background in this paper

  • ...In general, explants derived from micropropagated shoots have an early and greater capacity for morphogenesis than tissues excised from field plants ( George, 1996 ) which is attributed to absence of lag period between explanting and adaptation of explants to in vitro con-...

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Related Papers (5)
A revised medium for rapid growth and bio assays with tobacco tissue cultures

[...]

01 Jul 1962-Physiologia Plantarum
Toshio Murashige, Folke Skoog
Rapid propagation of Holostemma ada-kodien Schult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis

[...]

01 Aug 2002-Plant Cell Reports
K. P. Martin
In vitro micropropagation of Gymnema sylvestre - a multipurpose medicinal plant.

[...]

01 May 2000-Plant Cell Tissue and Organ Culture
N. Komalavalli, Mandali Venkateswara Rao
Rapid in vitro propagation of medicinally important Ceropegia candelabrum

[...]

01 Mar 2003-Plant Cell Tissue and Organ Culture
M.R. Beena, K. P. Martin, Pulugurtha Bharadwaja Kirti, Molly Hariharan 
Micropropagation of Hemidesmus indicus (L.) R. Br. through axillary bud culture

[...]

01 Feb 1996-Plant Cell Reports
J. Patnaik, B. K. Debata