Micropropagation of Indian wild strawberry.
01 Feb 2000-Plant Cell Tissue and Organ Culture (Kluwer Academic Publishers)-Vol. 60, Iss: 2, pp 83-88
TL;DR: An efficient method of micropropagation based on an increased percentage survival of explants and reduced phenol-induced browning in wild strawberry has been developed and 6-benzyladenine was found more effective than kinetin and N6-(γ,γ dimethylallyamino) purine.
Abstract: An efficient method of micropropagation based on an increased percentage survival of explants and reduced phenol-induced browning in wild strawberry has been developed. Serial transfer of nodal explants was carried out at 24-, 48- and 96-h intervals. Nodal segments cultured on Murashige and Skoog medium supplemented with 6-benzyladenine (4.0 μM) and α-naphthalene acetic acid (0.1 μM) gave the best (94.4%) explant establishment and shoot number (22.3) per explant. Of the cytokinins tested, 6-benzyladenine was found more effective than kinetin and N6-(γ,γ dimethylallyamino) purine. Excised shoots rooted on half-strength agar-gelled medium with 1.0 μM α-naphthalene acetic acid. Rooted shoots with fully expanded leaves acclimatized successfully and about 70% of plantlets survived ex vitro.
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TL;DR: Micropropagated plants established in garden soil, farmyard soil, and sand were uniform and identical to the donor plant with respect to growth characteristics as well as floral features.
Abstract: An efficient rapid and large-scale in vitro clonal propagation of the valuable medicinal herb Eclipta alba (Asteraceae) by enhanced axillary shoot proliferation in cotyledonary node segments was designed. The medium type, various carbon sources, plant growth regulators, and coconut water markedly influenced in vitro propagation of Eclipta alba. An in vitro plantlet production system has been investigated on Murashige and Skoog (MS) medium with the synergistic combination of benzyladenine (4.4μM), kinetin (4.6μM), 2-isopentenyladenine (4.9μM), gibberellic acid (1.4μM), 5% coconut water, and 3% sucrose which promoted the maximum number of shoots as well as beneficial shoot length: Subculturing of cotyledonary node segments on a similar medium enabled continuous production of healthy shoots with similar frequency. Rooting was highest (94.3%) on full strength. MS medium containing 9.8 μM indolebutyric acid. Micropropagated plants established in garden soil, farmyard soil, and sand (2∶1∶1) were uniform and identical to the donor plant with respect to growth characteristics as well as floral features. These plants grew normally without showing any morphological variation.
132 citations
TL;DR: Results indicated that these species should be promoted as a natural source of antioxidant/nutraceuticals so that these antioxidants can be used for supplementing dietary foods of mountain people.
72 citations
TL;DR: This paper describes an efficient in vitro micropropagation of Artemisia vulgaris using shoot tip and nodal explants and the survival rate of plantlets varied under acclimatization, the first report on plant regeneration via organogenesis of A. vulgaris.
Abstract: This paper describes an efficient in vitro micropropagation of Artemisia vulgaris using shoot tip and nodal explants. Among the various growth regulators tested, MS medium and B5 vitamins supplemented with BA (4.44 μM) and KN (2.32 μM) combination was found to yield a better response than BA (4.44–13.32 μM) or KN (0.46–13.92 μM) alone in the medium. BA and KN combinations produced a maximum of 23.3 shoots per explant with 99.8% shooting frequency. Multiple shoots raised were elongated on MS medium containing 0.44 μM BA and 1.44 μM GA3. Rooting was highest (98.2%) on MS medium containing 8.56 μM IAA. Rooted plantlets were successfully transferred to plastic cups containing autoclaved garden soil, farmyard soil and sand (2:1:1) for hardening. After 65 days, the plantlets were transferred to Botanical Evaluation Garden and maintained. The survival rate of plantlets varied under acclimatization. Plants looked healthy with no visually detectable phenotypic variations. This is the first report on plant regeneration via organogenesis of A. vulgaris.
59 citations
Additional excerpts
...A similar response was also observed by Indhra and Dhar (2000)....
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Journal Article•
48 citations
TL;DR: This study demonstrates for the first time that resistance of a strawberry cultivar to F. oxysporum f.
Abstract: Background and aims
Strawberry (Fragaria x ananassa) is a high-value crop worldwide. Fusarium oxysporum f. sp. fragariae causes rapid wilting and death of strawberry plants and severe economic losses worldwide. To date, no studies have been conducted to determine colonisation of either susceptible or resistant strawberry plants by F. oxysporum f. sp. fragariae, or whether plant colonisation by F. oxysporum f. sp. fragariae differs between susceptible and resistant cultivars.
46 citations
Cites methods from "Micropropagation of Indian wild str..."
...A tissue culture system was developed to aseptically produce seedlings from resistant and susceptible strawberry cultivars for this study based on methods described by Bhatt and Dhar (2000) and Sakila et al. (2007)....
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References
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12 Nov 1980
2,777 citations
TL;DR: Tanggal Ketik: 19930816, rd. nrd. nDokumentalis: ju. nmu as mentioned in this paper, Catatan Umum: Vol.1: Techniques for propagation and breeding; Vol.2and3:Crop species; Vol 4.5:ornamental species.
Abstract: Tanggal Ketik: 19930816, rd. nDokumentalis: ju. nmu. nrd. nCatatan Umum: Vol.1: Techniques for propagation and breeding; Vol.2and3:Crop species; Vol.5:ornamental species.
1,030 citations
01 Jan 1983
325 citations
"Micropropagation of Indian wild str..." refers background in this paper
...Hu and Wang (1983) reported that high concentration of cytokinin reduced the number of micropropagated shoots....
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TL;DR: It is estimated that by subculture 500 viable plants can be obtained from a single bud of a mature plant or 3000 plants from a seedling in a year.
143 citations
Additional excerpts
...…PVP (Walkey, 1972), distilled water (Vietez and Vietez, 1980), PVP + sucrose, antioxidant solution (Anderson, 1975; Ziv and Halvy, 1983), sucrose (Gupta et al., 1980) and chilling treatment at 4◦C (Dhar and Upreti, 1999) have been found to be helpful in reducing phenol-induced browning in a…...
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