Journal ArticleDOI
Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation.
Y. Sahoo,Pradeep K. Chand +1 more
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TLDR
Although callus-free multiple-shoot formation was a function of cytokinin activity alone, faster bud break coupled with an enhanced frequency of shoot development and internode elongation were dependent on the synergistic influence of gibberellic acid (GA3) along with BA when used at an optimal concentration.Abstract:
A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins – N6-benzyladenine (BA), kinetin, and thidiazuron – evaluated as supplements to Murashige and Skoog (MS) medium, BA at an optimal concentration of 2.0 mg/l was most effective in inducing bud break. Although callus-free multiple-shoot formation was a function of cytokinin activity alone, faster bud break coupled with an enhanced frequency of shoot development (92%) and internode elongation were dependent on the synergistic influence of gibberellic acid (GA3) when used at an optimal concentration (0.4 mg/l) along with BA (2.0 mg/l). The frequency of shoot proliferation was markedly influenced by the explanting season. By repeated subculturing of nodal segments harvested from the in vitro-formed axenic shoots on MS containing 1.0 mg/l BA and 0.4 mg/l GA3, prolific shoot cultures free from proximal callusing and showing a high-frequency multiplication rate were established. The percentage shoot multiplication (98–100%) as well as the number of shoots per node (six to eight) were highest during the first three culture passages, after which there was a gradual decline in shoot development. Rooting was best induced (94%) in shoots excised from proliferated shoot cultures on half-strength MS medium augmented with an optimal combination of indole-3-acetic acid and indole-3-butyric acid each at 1.0 mg/l. Vermi-compost was the most suitable planting substrate for hardening inside a plant growth chamber and its use ensured high-frequency survival (93%) of regenerated plants prior to outdoor transfer. Micropropagated plants established in garden soil were uniform and identical to the donor plant with respect to growth characteristics as well as vegetative and floral morphology.read more
Citations
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Journal ArticleDOI
Comparative studies of cytokinins on in vitro propagation of Bacopa monniera
TL;DR: In vitro derived shoots were elongated on growth regulator-free MS medium and exhibited better rooting response on medium containing 4.9 μM IBA and almost 100% transplantation success in the field.
Journal ArticleDOI
An efficient micropropagation system for Eclipta, alba —A valuable medicinal herb
Ponnusamy Baskaran,N. Jayabalan +1 more
TL;DR: Micropropagated plants established in garden soil, farmyard soil, and sand were uniform and identical to the donor plant with respect to growth characteristics as well as floral features.
Journal Article
Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization
Satish Manohar Nalawade,Abhay P. Sagare,Chen-Yue Lee,Chao-Lin Kao,Hsin-Sheng Tsay,Jen-Te Hsiang,Tainan Hsien +6 more
TL;DR: Advanced biotechnological methods of culturing plant cells and tissues should provide new means of conserving and rapidly propagating valuable, rare, and endangered medicinal plants.
Journal ArticleDOI
Micropropagation of Centella asiatica (L.), a valuable medicinal herb
TL;DR: Although bud break was dependent on BA supply, the synergistic combination of 22.2 μM BA and 2.68 μM NAA induced the optimum frequency of shoot formation as well as shoot number of Centella asiatica plants.
Journal ArticleDOI
An efficient micropropagation protocol for an endangered ornamental tree species ( Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers
Cui Yuanyuan,Cui Yuanyuan,Yanwen Deng,Keyuan Zheng,Xiaomin Hu,Mulan Zhu,Deng Xiaomei,Xi Ruchun +7 more
TL;DR: The described protocol for efficient regeneration of M. sirindhorniae can safely be applied for large scale propagation of this imperative plant.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Thidiazuron: a potent cytokinin for woody plant tissue culture
Carl A. Huetteman,John E. Preece +1 more
TL;DR: Thidizuron (TDZ) is among the most active cytokinin-like substances for woody plant tissue culture and facilitates efficient micropropagation of many recalcitrant woody species.
Journal ArticleDOI
The induction of flowering in vitro in stem segments of Plumbago indica L. : II. The production of reproductive buds.
Colette Nitsch,J. P. Nitsch +1 more
TL;DR: Internode segments excised from vegetative stems of Plumbago indica, an absolute short-day plant, can be induced to form in vitro a callus, or roots, or vegetative shoots, or inflorescences and flowers.
Journal ArticleDOI
Regeneration of apple plants from shoot meristem-tips
TL;DR: Pear plants were regenerated from shoot meristem-tips in vitro and Benzyladenine at 5 μM was optimal for shoot multiplication which was also enhanced by tip removal and horizontal or inverted orientation to decrease apical dominance.
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