Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
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TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
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Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania Somnifera (L.) Dunal. plantlets
TL;DR: Both the quality and the quantity of light affect growth of plantlets, development of stomata and physiological responses differently depending on the intensity and the wavelength of light.
Journal ArticleDOI
Micropropagation of Vitex negundo L., a woody aromatic medicinal shrub, through high-frequency axillary shoot proliferation.
Y. Sahoo,Pradeep K. Chand +1 more
TL;DR: Although callus-free multiple-shoot formation was a function of cytokinin activity alone, faster bud break coupled with an enhanced frequency of shoot development and internode elongation were dependent on the synergistic influence of gibberellic acid (GA3) along with BA when used at an optimal concentration.
Journal ArticleDOI
In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil).
TL;DR: A procedure is outlined for in vitro propagation of two medicinal herbs, OCimum americanum L. canum Sims (hoary basil) and Ocimum sanctum L.'souvenir (holy basil), using axillary shoot buds using benzyladenine supplemented with BA to induce multiple shoot formation.
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TL;DR: The investigation of a case of occupational silicosis in Ohio is described and the impact of hospital-based reporting on surveillance forsilicosis is summarized.
Journal ArticleDOI
In vitro callus induction and regeneration studies in Withania somnifera
G. Rani,I.S. Grover +1 more
TL;DR: Callus cultures were initiated from axillary leaves, axillary shoots, hypocotyls, and root segments on Murashige and Skoog medium supplemented with 2,4-D and KN to establish axillary shoot base callus on MS medium containing BA.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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