Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
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Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
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Journal Article
Development of an efficient method for regeneration and Agrobacterium -mediated transformation of cotton ( Gossypium hirsutum L.) cv. HS6
TL;DR: The results obtained with this reproducible protocol clearly demonstrated that transformation efficiency and regeneration of G. hirsutum cv.
Journal ArticleDOI
Influence of explanting season on in vitro multiplication of the medicinal herb, Tridax procumbens L.
C. P. Malik,Chitra Wadhwani +1 more
TL;DR: A protocol for complete regeneration of Tridax procumbens, a medicinally important plant, was accomplished through in vitro culturing and epicuticular contents correlated with the rate of cuticular transpiration.
Journal ArticleDOI
Callus culture and plant regeneration from seedling explants in ‘Poshita’ indian ginseng
Ajit Arun Waman,Umesha Konana,Sathyanarayana Bangalore Narayanappa,Ashok Tholakalabavi,Balakrishna Gowda +4 more
TL;DR: Indole-3-butyric acid (IBA) was found to be superior source of auxin for all rooting parameters, except percent rooting, and plantlets were best hardened when agropeat was used as a substrate.
Journal ArticleDOI
In vitro and in situ screening systems for morphological and phytochemical analysis of Withania somnifera germplasms
TL;DR: This report reports, for the first time, the use of a modified in vitro system for morphological and phytochemical screening of true to type plants as compared with those grown in a conventional in situ system for Withania somnifera.
Micro propagation and tissue culture of the endangered medicinal plant withania somnifera by the direct shoot and root initiation method
A. Arumugam,K. Gopinath +1 more
TL;DR: This new protocol was standardized for easy mass propagation of W. somnifera medicinal plant and found callus initiation was observed best in MS media with (2,4- D 1.0-5.0 mg/L) after 16-20 days (93%).
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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