Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
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TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
Citations
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Journal ArticleDOI
Micropropagation and Conservation of Rheum webbianum Collected from Zanaskar Valley via Tissue Culture
TL;DR: The Rheum webbianum was conserved by the tissue culture and showed best results on different phyrohormones and was observed on combination of BAP, TDZ, 2,4-D and IBA.
Journal Article
Micropropagation of Valeriana wallichii DC. (Indian Valerian) through nodes.
TL;DR: High frequency shoot proliferation was induced in explants cultured on Murashige and Skoog medium supplemented with different concentrations of 6-benzyladenine (BA), kinetin (Kn), adenine sulphate (AS) and thidiazuron (TDZ).
Journal Article
Plant regeneration from mature cotyledon, embryo and hypocotyl explants of Withania somnifera (L.) Dunal.
TL;DR: In terms of in vitro genotypic response genotype JA-20 was found significantly superior to MWS-100 for the most of the attributes investigated, and Regenerated plantlets were established successfully in the field after hardening.
Dissertation
Micropropagation and storage of Plectranthus amboinicus (Loureiro) Sprengel shoot apices for germplasm conservation
TL;DR: A reproducible micro-propagation method and a complimentary conservation strategy for a sustainable utilisation of P. amboinicus is presented in this article. But the method is not suitable for propagation and conservation of tropical medicinal plants.
Journal ArticleDOI
In vitro propagation of d ipteris wallichii (r. br .) t. moore : a hope fo r conservation of an endangered pteridophyte
TL;DR: In vitro protocol for micro propagation of D. wallichii Moore was carried out and it was observed that supplemen tation of phytohormones mainly auxins were favourable for sporophyte development from prothallus.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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