Journal ArticleDOI
Micropropagation of Withania somnifera from germinating seeds and shoot tips
Jayanti Sen,Archana Sharma +1 more
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TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.Abstract:
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.read more
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Journal Article
An efficient protocol for shoot bud induction and regeneration of Tylophora indica, an endangered medicinal plant
TL;DR: Among the four media and three explants employed, MS medium followed by LS, B5 and L6 (MS>LS>B5>L6) and leaf followed by shoot and root resulted in higher frequency (90%) of shoot bud formation and maximum number of mean shoot buds (16.2).
Journal Article
In Vitro Micropropagation of Carum copticum L.
Jaydip M,Poornima Sharma +1 more
TL;DR: The clonal propagation protocol established from seedling explants of Carum copticum can be successfully employed for large scale production of sterile plants for pharmaceutical use and genetic transformation studies.
Dissertation
ROLE OF NITRIC OXIDE AND POLYAMINE IN AMELIORATING DROUGHT STRESS IN Stevia rebaudiana Bertoni UNDER IN VITRO CONDITION WITH SPECIAL REFERENCE TO BIOCHEMICAL AND MOLECULAR CHANGES
TL;DR: This paper presents a meta-analyses of the chiral stationary phase phytochemical analysis technique and its applications in plant Physiology and shows promising results.
STUDIES ON TISSUE CULTURE OF ASHOKA (Saraca indica L.) AND ASHWAGANDHA (Withania somnifera L.)
TL;DR: Investigation of culture of ashoka and standardizing efficient micropropagation protocol in ashwagandha found NAA was found to be superior in callus induction compared to 2,4-D, and callus was formed as intermediate stage during direct shoot regeneration.
Journal ArticleDOI
An overview of biotechnological interventions and abiotic elicitors on biomass and withanolide biosynthesis in Withania somnifera (L.) Dunal
TL;DR: In this paper , a review of the research works performed for enhanced biomass production and to scale-up the synthesis of withanolides by employing biotechnological tools, abiotic elicitors for growth and enhanced metabolism in W. somnifera is presented.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI
Primary and secondary metabolism of plant cell cultures
TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI
Chemistry and genetics of withanolides in Withania somnifera hybrids
S. Sarma Nittala,David Lavie +1 more
TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI
Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)
TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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