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Journal ArticleDOI

Micropropagation of Withania somnifera from germinating seeds and shoot tips

Jayanti Sen, +1 more
- 01 Aug 1991 - 
- Vol. 26, Iss: 2, pp 71-73
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TLDR
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. with direct multiple shoot initiation from germinating seeds in the presence of BA alone.
Abstract
Shoot multiplication was achieved in vitro from shoot tips of aseptically germinated seedlings of Withania somnifera L. using low concentrations of 6-benzyladenine (BA), viz. 2.2, 4.4 and 8.9 μM. Maximum number of shoots were obtained when 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 2.5 μM indolebutyric acid (IBA) was added to medium containing 4.4 μM BA during initiation of shoot multiplication, but not when added later. Direct multiple shoot initiation was also obtained from germinating seeds in the presence of BA alone. Rooting was successful in excised shoots grown on growth regulator-free MS medium. Rooted shoots were successfully established in soil in a greenhouse.

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Citations
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Anatomy study of shoot tips, shoots, flowers and immature fruits of Salvadora persica in vitro

TL;DR: Shoot tips, Shoots, flowers and immature fruits were used as explants, inoculated on MS medium supplemented with different concentrations of some growth hormones and small size of parenchyma cells surrounded by thin cell wall were observed.
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In vitro micropropagation of withania somnifera (l.) dunal by different concentrations of growth regulators

TL;DR: In this article, the authors propose a framework to improve the quality of the data collected by the data collection system. But they do not specify how to collect the data from all the data sources.
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Effect of Supplementing Mixture of Benzene Amino purine (BAP) and Kinetin (KN) Along with Auxin 2,4 D On Growth Of Callus In Murashige And Skoog Medium Derived From Embryonic Cotyledon Explants of Withania Somnifera L

TL;DR: Withania somnifera (L.) Dunal (Family: Solanaceae, commonly known as Ashwagandha, English name: Winter cherry) is an important perennial plant species with immense therapeutic uses in traditional as well as modern system of medicine

In vitro Propagation of Trichosanthes Cucumerina L. (Snake tomato)

TL;DR: Generally BA was superior in proliferation capacity as compared to KIN or BA in combination with KIN, and poorly developed roots or no rooting was observed on almost all the media enriched with a combination of both BA and kinetin.
References
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Journal ArticleDOI

A revised medium for rapid growth and bio assays with tobacco tissue cultures

TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
BookDOI

Primary and secondary metabolism of plant cell cultures

TL;DR: From Metabolism and Osmotic Work to Totipotency and Morphogenesis: A Study of Limitations Versus Multiple Interactions.
Journal ArticleDOI

Chemistry and genetics of withanolides in Withania somnifera hybrids

TL;DR: In this paper, the inheritance characteristics of various substituents on the withanolide skeleton were analyzed based on the occurrence in per cent of each substituent in relation to the total withanolides content in the hybrid plants and their respective parents.
Journal ArticleDOI

Factors affecting in vitro proliferation and rooting of shoots of jackfruit ( Artocarpus heterophyllus Lam.)

TL;DR: Of the cytokinins tested, benzylaminopurine (BAP) was more effective than either 2-isopentenyladenine (2iP) or kinetin (Kin) and produced maximum proliferation when used at 5×10-6M.
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