MicroRNAs in plasma of pancreatic ductal adenocarcinoma patients as novel blood-based biomarkers of disease
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...The combined expression analyses of miR-21 , miR-210 , miR-155 , and miR-196a in plasma can discriminate pancreatic adenocarcinoma patients from controls...
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Cites background from "MicroRNAs in plasma of pancreatic d..."
...Binderup et al. have emphasized the importance of the chosen normalization strategy in RT-qPCR data analyses [303]....
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...For the second step of the technique, real-time PCR is used to quantify the miRNAs targeted [95]....
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...Also, in patients with mild cognitive impairment (MCI), their predictive values are still to be determined [278]. miRNAs on the other hand display higher sensitivity levels in comparison to protein biomarkers, mainly due to PCR amplification [279]....
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...The gold-standard for miRNA quantification is quantitative reverse transcriptase PCR (RT-qPCR) [91–93]....
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...Due to the many qualities of RT-qPCR, which include high sensitivity, specificity, accessibility, and reproducibility, RT-qPCR is currently the most used technique, remaining the gold standard method for the verification of microarray and NGS results....
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References
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