Journal ArticleDOI
Microsatellites: simple sequences with complex evolution
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Few genetic markers, if any, have found such widespread use as microsatellites, or simple/short tandem repeats, but features such as hypervariability and ubiquitous occurrence explain their usefulness, but these features also pose several questions.Abstract:
Few genetic markers, if any, have found such widespread use as microsatellites, or simple/short tandem repeats. Features such as hypervariability and ubiquitous occurrence explain their usefulness, but these features also pose several questions. For example, why are microsatellites so abundant, why are they so polymorphic and by what mechanism do they mutate? Most importantly, what governs the intricate balance between the frequent genesis and expansion of simple repetitive arrays, and the fact that microsatellite repeats rarely reach appreciable lengths? In other words, how do microsatellites evolve?read more
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Journal ArticleDOI
Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers
TL;DR: This synthesis presents a multistep screening process to evaluate candidate loci for inclusion in a genetic study that is broadly targeted to both novice and experienced geneticists alike and aims to encourage the use and consistent reporting of thorough marker screening to ensure high quality data.
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Single-cell sequencing-based technologies will revolutionize whole-organism science
TL;DR: The unabated progress in next-generation sequencing technologies is fostering a wave of new genomics, epigenomics, transcriptomics and proteomics technologies, enabling high-throughput, multi-dimensional analyses of individual cells that will produce detailed knowledge of the cell lineage trees of higher organisms, including humans.
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Microsatellite markers: an overview of the recent progress in plants
TL;DR: The recent developments in plant genetics using SSR markers are discussed and a quantum of literature has accumulated regarding the applicability of SSR based techniques.
Journal ArticleDOI
Current trends in microsatellite genotyping.
Erwan Guichoux,Lélia Lagache,Stefanie Wagner,Stefanie Wagner,Philippe Chaumeil,Patrick Léger,Olivier Lepais,Olivier Lepais,Camille Lepoittevin,Thibaut Malausa,E. Revardel,Franck Salin,Rémy J. Petit +12 more
TL;DR: To make the most of the latest technical developments, the need for a well‐established strategy including standardized high‐throughput bench protocols and specific bioinformatic tools, from primer design to allele calling is outlined.
Journal ArticleDOI
Genetics and genomics of core short tandem repeat loci used in human identity testing.
TL;DR: The physical location of each STR locus in the human genome is delineated and allele ranges and variants observed in human populations are summarized as are mutation rates observed from parentage testing.
References
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TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
Journal ArticleDOI
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TL;DR: The results of an international collaboration to produce a high-quality draft sequence of the mouse genome are reported and an initial comparative analysis of the Mouse and human genomes is presented, describing some of the insights that can be gleaned from the two sequences.
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Journal Article
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James L. Weber,P E May +1 more
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Journal ArticleDOI
Hypervariability of simple sequences as a general source for polymorphic DNA markers
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