Mikromethode zur Bestimmung der Ringstruktur des Zuckerrestes bei Nucleosiden
TL;DR: In this paper, a new Mikromethode zur bestimmung der ringstruktur der Zucker von Nucleosiden wird beschrieben, which beruht auf der Oxydation der Zuckerkette mit Perjodsaure, Reduktion des entstandenen Dialdehyds with NaBH4 and anschliessender Hydrolyse with HCl.
Abstract: Eine neue Mikromethode zur Bestimmung der Ringstruktur der Zucker von Nucleosiden wird beschrieben. Die Methode beruht auf der Oxydation der Zuckerkette mit Perjodsaure, Reduktion des entstandenen Dialdehyds mit NaBH4 und anschliessender Hydrolyse mit HCl. Die dabei freigesetzten Alkohole werden papierchromatographisch nachgewiesen.
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TL;DR: The results indicate that these ribose-modified nucleosides and nucleotides can be versatile fluorescent substrate analogs for various enzymes.
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TL;DR: In the course of investigations into the application of paper chromatography to the identification of reducing substances in urine, it was found that, when ammoniacal silver nitrate was used as a developing reagent, urates produced a dark brown spot with an RF value of approximately 0.20.
Abstract: IN the course of investigations into the application of paper chromatography to the identification of reducing substances in urine it was found that, when ammoniacal silver nitrate was used as a developing reagent, urates, normally present in urine, produced a dark brown spot with an RF value of approximately 0·20. This spot, which generally ‘tails', tends to mask the spots produced by other reducing substances with RF values in the region of 0·20, namely, those of glucose, sorbose, arabinose and fructose.
127 citations
TL;DR: An investigation of the exudate of the leaves of Euonymus japonica by partition chromatography on strips of filter paper revealed that dulcitol gives rise to a discrete dark brown spot on the paper chromatogram when it is sprayed with an ammoniacal solution of silver nitrate and heated.
Abstract: BAKER1 has described the isolation of dulcitol, a non-reducing sugar alcohol, from the exudate of the leaves of Euonymus japonica. An investigation of this exudate by partition chromatography on strips of filter paper, employing the usual solvents for sugar analysis, revealed that dulcitol gives rise to a discrete dark brown spot on the paper chromatogram when it is sprayed with an ammoniacal solution of silver nitrate and heated. Other polyhydric alcohols, such as mannitol, sorbitol, glycerol and ethylene glycol, also reduce ammoniacal silver nitrate under these conditions, a most surprising and unpredictable result. In view of the importance of partition chromatography for the analysis of intimate mixtures of the reducing sugars2, an examination of the properties of the polyhydric alcohols on the paper chromatogram was necessary in order to avoid any confusion between these closely related substances. It is of interest to note that Partridge3 has encountered inositol, a cyclic hexitol, along with glucose and fructose in an extract of the fœtal blood of sheep, since he found that it also gives rise to a discrete brown spot on the paper chromatogram with the ammoniacal silver nitrate reagent.
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TL;DR: Aus Tetracetyl-D-ribose vom Smp. 110° wurde Acetobrom-Dribose hergestellt und letztere mit Nicotinsaureamid zum kristallisierten 3-Carbonsaure amid-N1D-triacetyl-ribosido-pyridiniumbromid umgesetzt.
Abstract: Aus Tetracetyl-D-ribose vom Smp. 110° wurde Acetobrom-D-ribose hergestellt und letztere mit Nicotinsaureamid zum kristallisierten 3-Carbonsaureamid-N1-D-triacetyl-ribosido-pyridiniumbromid umgesetzt. Die Verseifung dieser Verbindung mit Bromwasserstoffsaure fuhrte zum kristallisierten 3-Carbonsaureamid-N1-D-ribopyranosido-pyridiniumbromid (III); als Nebenprodukt wurde ein Monoacetat dieser Verbindung erhalten. Das 3-Carbonsaureamid-N1-D-ribopyranosido-pyridiniumbromid liess sich mittels Metaphosphorsaure in einen Triphosphorsaureester uberfuhren, aus dem durch partielle Verseifung ein Monophosphorsaureester erhalten wurde.
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