Journal ArticleDOI
Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective
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TLDR
A miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective is presented and fluorescent light was detected through the fiber bundle.Abstract:
We present a miniature, flexible two-photon microscope consisting of a fused coherent optical fiber bundle with 30,000 cores and a gradient-index lens objective. The laser focus of a standard two-photon laser-scanning microscope was scanned over the entrance surface of the fiber bundle, resulting in sequential coupling into individual cores. Fluorescent light was detected through the fiber bundle. Micrometer-sized fluorescent beads and pollen grains were readily resolved. In addition, fluorescently labeled blood vessels were imaged through the fiber bundle in rat brain in vivo.read more
Citations
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Journal ArticleDOI
Deep tissue two-photon microscopy
Fritjof Helmchen,Winfried Denk +1 more
TL;DR: Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed.
Journal ArticleDOI
Next-Generation Optical Technologies for Illuminating Genetically Targeted Brain Circuits
Karl Deisseroth,Guoping Feng,Ania K. Majewska,Gero Miesenböck,Alice Y. Ting,Mark J. Schnitzer +5 more
TL;DR: Key recent advances that unite optical and genetic approaches are explored, focusing on promising techniques that either allow novel studies of neural dynamics and behavior or provide fresh perspectives on classic model systems.
Journal ArticleDOI
Fiber-optic fluorescence imaging.
Benjamin A. Flusberg,Eric D. Cocker,Wibool Piyawattanametha,Juergen C. Jung,Eunice L. M. Cheung,Mark J. Schnitzer +5 more
TL;DR: Two new classes of photonic crystal fiber facilitate ultrashort pulse delivery for fiber-optic two-photon fluorescence imaging and an upcoming generation of fluorescence Imaging devices will be based on microfabricated device components.
Patent
Catheterscope 3D Guidance and Interface System
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Journal ArticleDOI
Imaging in vivo : watching the brain in action
Jason N. D. Kerr,Winfried Denk +1 more
TL;DR: The limits of what can be achieved in vivo have been pushed into terrain that was previously only accessible in vitro, due to advances in both physical-imaging technology and the design of molecular contrast agents.
References
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Journal ArticleDOI
Photon Upmanship: Why Multiphoton Imaging Is More than a Gimmick
Winfried Denk,Karel Svoboda +1 more
TL;DR: The unique niche that light microscopy occupies in biology is based on the ability to perform observations on living tissue at relatively high spatial resolution, but this resolution is limited by the wavelength of light and does not rival that of electron microscopy.
Journal ArticleDOI
Two-photon imaging to a depth of 1000 µm microm in living brains by use of a Ti:Al2O3 regenerative amplifier
TL;DR: It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses.
Journal ArticleDOI
A Miniature Head-Mounted Two-Photon Microscope: High-Resolution Brain Imaging in Freely Moving Animals
TL;DR: This work extends two-photon imaging from anesthetized, head-stabilized to awake, freely moving animals by using a miniaturized head-mounted microscope and readily obtained images of vasculature filled with fluorescently labeled blood and of layer 2/3 pyramidal neurons filled with a calcium indicator.
Journal ArticleDOI
In Vivo Multiphoton Microscopy of Deep Brain Tissue
TL;DR: It is shown that multiphoton microscopy through GRIN lenses enables minimally invasive, subcellular resolution several millimeters in the anesthetized, intact animal, and it is presented in vivo images of cortical layer V and hippocampus in theAnesthetizing Thy1-YFP line H mouse.
Journal ArticleDOI
Multiphoton endoscopy: optical design and application to in vivo imaging of mammalian hippocampal neurons
TL;DR: This work introduces minimally invasive multiphoton fluorescence microendoscopes (350-1000 /spl mu/ in diameter) based on compound microlenses and demonstrates multipoton endoscopy in live animals by visualizing rodent hippocampal neurons and dendrites.