Journal ArticleDOI
miR-486-3p regulates CyclinD1 and promotes fluoride-induced osteoblast proliferation and activation.
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TLDR
In this article, the role and specific molecular mechanism of miR-486-3p in fluoride-induced osteoblast proliferation and activation via CyclinD1 was investigated.Abstract:
Fluoride is a persistent environmental pollutant, and its excessive intake contributes to skeletal and dental fluorosis The mechanisms underlying fluoride-induced abnormal osteoblast proliferation and activation, which are related to skeletal fluorosis, have not yet been fully clarified As important epigenetic regulators, microRNAs (miRNAs) participate in bone metabolism On the basis of our previous miRNA-seq results and bioinformatics analysis, this study investigated the role and specific molecular mechanism of miR-486-3p in fluoride-induced osteoblast proliferation and activation via CyclinD1 Herein, in the fluoride-challenged population, we observed that miR-486-3p expression decreased while CyclinD1 and transforming growth factor (TGF)-β1 increased, and miR-486-3p level correlated negatively with the expression of CyclinD1 and TGF-β1 genes Further, we verified that sodium fluoride (NaF) decreases miR-486-3p expression in human osteoblasts and overexpression of miR-486-3p reduces fluoride-induced osteoblast proliferation and activation Meanwhile, we demonstrated that miR-486-3p regulates NaF-induced upregulation of CyclinD1 by directly targeting its 3'-untranslated region (3'-UTR) In addition, we observed that NaF activates the TGF-β1/Smad2/3/CyclinD1 axis and miR-486-3p mediates transcriptional regulation of CyclinD1 by TGF-β1/Smad2/3 signaling pathway via targeting TGF-β1 3'-UTR in vitro This study, thus, contributes significantly in revealing the mechanism of miR-486-3p-mediated CyclinD1 upregulation in skeletal fluorosis and sheds new light on endemic fluorosis treatmentread more
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Journal ArticleDOI
Progress of Signaling Pathways, Stress Pathways and Epigenetics in the Pathogenesis of Skeletal Fluorosis.
Lichun Qiao,Xuan Liu,Yujie He,Jiaheng Zhang,Hao Huang,Wenming Bian,Mumba Mulutula Chilufya,Yan Zhao,Jing Han +8 more
TL;DR: In this paper, a comprehensive review of the pathogenesis of skeletal fluorosis is presented, which summarizes and analyzes relevant findings to provide a basis for comprehensive understandings of the disease and propose more effective prevention and therapeutic strategies.
Journal ArticleDOI
Biphasic Functions of Sodium Fluoride (NaF) in Soft and in Hard Periodontal Tissues
Xingzhi Wang,Nitesh Tewari,Fuyuki Sato,Keiji Tanimoto,Lakshmi Thangavelu,Makoto Makishima,Ujjal K. Bhawal +6 more
TL;DR: Recent studies on the biphasic functions of NaF that are related to both soft and hard periodontal tissues, multiple diseases, and clinical dentistry are summarized.
Journal ArticleDOI
A systematic review on fluoride-induced epigenetic toxicity in mammals
TL;DR: In this article , a systematic review summarizes the current knowledge regarding fluoride-induced epigenetic toxicity in the in vitro, in vivo, and epidemiological studies in mammalian systems, and concludes that further studies are warranted to elucidate and confirm the mechanism of epigenetic alterations mediated fluoride toxicity.
Journal ArticleDOI
Fluoride in the Central Nervous System and Its Potential Influence on the Development and Invasiveness of Brain Tumours—A Research Hypothesis
TL;DR: A review of the role of fluoride in the pathogenesis of brain tumours, including glioblastoma (GBM), is presented in this article . But, the authors focus on the potential role of fluorine compounds in the development and progression of GBM.
Journal ArticleDOI
Endoplasmic reticulum chaperone GRP78 participates in fluoride‐induced autophagy in LS8 cells by regulating the IRE1‐TRAF2‐JNK pathway
TL;DR: In this article , the role of the ER molecular chaperone GRP78 in the regulation of autophagy in ameloblast LS8 cells was investigated and the relationship between fluoride-induced endoplasmic reticulum (ER) stress and autophagia was explored.
References
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TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation
TL;DR: Recent advances in molecular and genetic studies using gene targeting in mice enable a better understanding of TGF-β/BMP signaling in bone and in the signaling networks underlying osteoblast differentiation and bone formation.
Journal ArticleDOI
Fluoride contamination, health problems and remediation methods in Asian groundwater: A comprehensive review.
Krishna Kumar Yadav,Sandeep Kumar,Quoc Bao Pham,Neha Gupta,Shahabaldin Rezania,Hesam Kamyab,Shalini Yadav,Jan Vymazal,Vinit Kumar,Doan Quang Tri,Amirreza Talaiekhozani,Shiv Prasad,Lisa M. Reece,Neeraja Singh,Pradip Kumar Maurya,Jinwoo Cho +15 more
TL;DR: Different methods to remove fluoride from water such as reverse osmosis, electrocoagulation, nanofiltration, adsorption, ion-exchange and precipitation/coagulating were introduced in this study.
Journal ArticleDOI
Cyclin D1 and CDK4 Activity Contribute to the Undifferentiated Phenotype in Neuroblastoma
Jan J. Molenaar,Marli E. Ebus,Jan Koster,Peter van Sluis,Carel J. M. van Noesel,Rogier Versteeg,Huib N. Caron +6 more
TL;DR: It is reported that analysis of Affymetrix expression data of primary neuroblastic tumors shows an extensive overexpression of Cyclin D1, which correlates with histologic subgroups and concludes that neuroblastoma functionally depend on overeexpression of G(1)-regulating genes to maintain their undifferentiated phenotype.
Journal ArticleDOI
The pathogenesis of endemic fluorosis: Research progress in the last 5 years.
TL;DR: In the past 5 years, many researchers have conducted in‐depth studies into the pathogenesis of endemic fluorosis and research in the areas of fluoride‐induced stress pathways, signalling pathways and apoptosis has provided further extensive knowledge at the molecular and genetic level.
Journal ArticleDOI
Outline of control practice of endemic fluorosis in China
Wang Lian-Fang,Huang Jian-Zhong +1 more
TL;DR: The incidence of the illness was reduced with some patients making a complete recovery after all the control programmes had been introduced, and many projects for improving drinking water quality through de-fluoridation have been completed.