Misidentification of Brucella melitensis as Bergeyella zoohelcum by MicroScan WalkAway®: a case report
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TLDR
This case showed that Brucella can be misidentified using MicroScan WalkAway, and countries where brucellosis is endemic need to be careful while using such automated identification systems in order not to miss the diagnosis of brucella.Abstract:
Objective: To describe the misidentification of Brucella melitensis as Bergeyella zoohelcum by MicroScan WalkAway®, a commonly used bacterial identification system. Clinical Presentation and Intervention: A 35-year-old man was admitted to the Intensive Care Unit with sepsis syndrome. Three sets of aerobic blood culture samples were positive after 48 h of incubation. The isolated organism was identified as B. zoohelcum using the MicroScan WalkAway (Siemens Healthcare Diagnostics Inc., West Sacramento, Calif., USA). However, due to the rareness of the pathogen, the isolate was reidentified as B. melitensis with Vitek® 2 system and later 16S ribosomal sequence analysis confirmed the isolate as B. melitensis having 100% match. Conclusion: This case showed that Brucella can be misidentified using MicroScan WalkAway. Countries where brucellosis is endemic need to be careful while using such automated identification systems in order not to miss the diagnosis of Brucella.read more
Citations
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Misidentification of Brucella melitensis as Ochrobactrum anthropi by API 20NE
TL;DR: Brucella organisms have been misidentified as Moraxella phenylpyruvica by the API 20NE non-enteric identification system (bioMérieux) and the importance of differentiation from other cases of pseudobacteraemia caused by O. anthropi is highlighted.
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Ribosomal RNA Sequence Analysis of Brucella Infection Misidentified as Ochrobactrum anthropi Infection
TL;DR: A Brucella isolate was identified from purulent material collected during a hip surgery, and two previous blood cultures from the same patient yielded Ochrobactrum anthropi, which was identified as BrucellA suis after rRNA sequencing.