Mitochondrial Redox Metabolism in Trypanosomatids Is Independent of Tryparedoxin Activity
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Cites background from "Mitochondrial Redox Metabolism in T..."
...The class II Tpxs are unlikely to function as normal Tpxs owing to a number of amino acid substitutions (41)....
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...infantum (214), but the mTpx homozygous knockout promastigotes do not show any growth retardation or morphological alterations when compared to wild-type parasites (41)....
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Cites background from "Mitochondrial Redox Metabolism in T..."
...Moreover, the proposed model for kDNA replication established a fundamental role for a mitochondrial TXN as reductant of UMSBP and it was shown previously that such enzyme is not essential in Leishmania and does not exist in trypanosomes [36]....
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...This hypothesis requires that the substrate specificities of nsGPX and mTXNPx overlap [33–35], and implies the existence of an efficient nsGPX reductant in the mitochondrion, which is still to be found [36]....
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References
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"Mitochondrial Redox Metabolism in T..." refers methods in this paper
...Phylogenetic trees were constructed by the MEGA4 program [29]....
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...the MEGA4 software [29], several methods were employed to...
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2,383 citations
"Mitochondrial Redox Metabolism in T..." refers background in this paper
...By analogy with thioredoxins, such redox processes could include protein folding assistance, protease activation and intracellular signaling [42,43]....
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864 citations
"Mitochondrial Redox Metabolism in T..." refers methods in this paper
...In the classical Holmgren assay [24], 6HisLiDTXN3 proved to...
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538 citations
"Mitochondrial Redox Metabolism in T..." refers background in this paper
...Some of these proteins include components of the translocation machinery of the mitochondria [39], as well as mitochondrial outer membrane proteins that regulate apoptosis [40] and mitochondrial morphology [41]....
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339 citations
"Mitochondrial Redox Metabolism in T..." refers background in this paper
...It is generally accepted that Trypanosoma TXN2 genes encode the mitochondrial TXN of these organisms [1,16,17]....
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...The Ser at this position is present in all enzymes with known TXN activity and is in the vicinity of an acidic area proposed to be important for TXN interaction with 2-Cys PRXs [1]....
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...to a variety of thiol-containing molecules [1]....
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...Rather it results from the accumulation of several other substitutions, which are also present in the TXN2 sequences of Trypanosoma....
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...Accordingly, it is reasonable to assume that the trypanosome enzymes also display deficient TXN activity....
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