Mitotic Exit in the Absence of Separase Activity
Citations
3 citations
Cites background from "Mitotic Exit in the Absence of Sepa..."
...However, there is a second activator of APC, Cdh1, which normally stimulates the APC at the very end of the cell cycle [68, 70, 72, 77, 157-159]....
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2 citations
Cites background from "Mitotic Exit in the Absence of Sepa..."
...extrusion, and dynamics of the anaphase spindle (Kudo et al., 2006; Lu and Cross, 2009; Sullivan and Uhlmann, 2003)....
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...Other non-proteolytic functions of separase have been identified, such as control of mitotic exit, polar body extrusion, and dynamics of the anaphase spindle (Kudo et al., 2006; Lu and Cross, 2009; Sullivan and Uhlmann, 2003)....
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1 citations
Cites methods or result from "Mitotic Exit in the Absence of Sepa..."
...Those pixels, if scored, were excluded from quantification, but did not cause any difference to our conclusions(Lu and Cross, 2009)....
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...…of cellular Cdc14-YFP pixel intensities, standardized to variation of nucleolar Net1-mCherry, which is an improved version of my previous assay (Lu and Cross, 2009) (this Cdc14 release measure is mathematically predicted to vary linearly with the fraction of Cdc14 released into cytoplasm,…...
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...I designed a quantitative, single cell measurement for Cdc14 localization based on variation of cellular Cdc14-YFP pixel intensities, standardized to variation of nucleolar Net1-mCherry, which is an improved version of my previous assay (Lu and Cross, 2009) (this Cdc14 release measure is mathematically predicted to vary linearly with the fraction of Cdc14 released into cytoplasm, Materials and Methods)....
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1 citations
Cites methods from "Mitotic Exit in the Absence of Sepa..."
...Using the protocol from the Cross lab (Lu and Cross, 2009; Bloom et al., 2011), I was able to arrest cells at a stage in the cell cycle that was...
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...JA256 (cdc20::MET-CDC20-TRP1 cdh1Δ::LEU2 esp1::GALS-ESP1-URA3 SPC42GFP-KANMX ade2::ADE2) was created via mating of YL165 (cdc20::MET-CDC20-TRP1 cdh1::LEU2 ESP1::GALS-ESP1-URA3 CDC14-YFP-HIS3 NET-CFP-KAN MATa; gift of Fred Cross) (Lu and Cross, 2009) and SFI1-NATMX SPC42-GFP-KANMX ade2::ADE2 MATα...
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...I thank John Kilmartin, Orna Cohen-Fix, Fred Cross, Ivan Rayment, Kevin Hardwick, Greg Odorizzi, Stanley Fields, and the Yeast Resource Center for sharing strains, plasmids and/or reagants....
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References
5,176 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...We confirmed that Pds1 was not degraded despite expression of GAL-SIC1-4A (in fact, its level increased [Supplemental Figure 1C], perhaps due to increased transcription after mitosis [Spellman et al., 1998])....
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...developed by Sullivan and Uhlmann (2003). Cells were depleted of Cdc20 and arrested in metaphase, by using a methionine-suppressible MET3-CDC20 construct (Sullivan and Uhlmann, 2003)....
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1,545 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...The temperature-sensitive scc1-73 cohesin allele (Michaelis et al., 1997) allows sister chromatid separation without Esp1 activity at restrictive temperature (Uhlmann et al....
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...The temperature-sensitive scc1-73 cohesin allele (Michaelis et al., 1997) allows sister chromatid separation without Esp1 activity at restrictive temperature (Uhlmann et al., 1999)....
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1,021 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...The temperature-sensitive scc1-73 cohesin allele (Michaelis et al., 1997) allows sister chromatid separation without Esp1 activity at restrictive temperature (Uhlmann et al., 1999)....
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...At 37°C, 35% initially unbudded cells elongated the spindle in spite of galactose addition, suggesting inefficient expression of GAL1-SCC1-RRDD at 37°C....
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...A MYO1-GFP TUB1-GFP strain lacking GAL1-SCC1-RRDD (BD78-2C) was treated in parallel as a control, pooling bud-to-bud data for mothers and daughters....
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...In these experiments, blocking sister chromatid separation does not block Esp1 activation, because endogenous Scc1 is cleaved on schedule even in the presence of ectopic Scc1RRDD (Uhlmann et al., 1999)....
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...This question has been examined previously with the use of the noncleavable version of Scc1 expressed from the GAL1 promoter (GAL1-SCC1-RRDD) (Uhlmann et al., 1999)....
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1,011 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...The potential lethality of this allele was overcome by mildly overexpressing Esp1 under a truncated GAL1 promoter (Mumberg et al., 1994), GALS-ESP1....
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951 citations
"Mitotic Exit in the Absence of Sepa..." refers background in this paper
...DNA replication in the next cell cycle did not occur, presumably because of stable Sic1 accumulation ( Schwob et al., 1994; Verma et al., 1997)....
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...DNA replication in the next cell cycle did not occur, presumably because of stable Sic1 accumulation (Schwob et al., 1994; Verma et al., 1997)....
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