Mitotic Exit in the Absence of Separase Activity
Ying Lu,Frederick R. Cross +1 more
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TLDR
The first quantitative measure for Cdc14 release based on colocalization with the Net1 nucleolar anchor is defined, indicating efficient CDC14 release upon MEN activation; release driven by Esp1 in the absence of microtubules was inefficient and incapable of driving ME.Abstract:
In budding yeast, three interdigitated pathways regulate mitotic exit (ME): mitotic cyclin–cyclin-dependent kinase (Cdk) inactivation; the Cdc14 early anaphase release (FEAR) network, including a n...read more
Citations
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Book ChapterDOI
Studying the Role of the Mitotic Exit Network in Cytokinesis.
TL;DR: Methods to induce cytokinesis after the inactivation of CDK activity in temperature sensitive mutants of the MEN pathway are presented and an array of methods to monitor the cellular events associated with the successful onset of cytokinesIS is included.
Dissertation
Mathematical Modelling of Mitotic Exit Control in Budding Yeast Cell Cycle
TL;DR: A comprehensive mathematical model is presented, which provides a system’s-level understanding of the mitotic exit process, and overcomes a number of limitations present in previous models.
Journal ArticleDOI
Characterization of a novel separase-interacting protein and candidate new securin, Eip1p, in the fungal pathogen Candida albicans .
TL;DR: The results introduce a candidate new securin, provide an approach for identifying these divergent proteins, reveal a putative anti-fungal therapeutic target, and highlight variations in mitotic regulation in eukaryotes.
Posted ContentDOI
Methods to study toxic transgenes in C. elegans: an analysis of protease-dead separase in the C. elegans embryo
TL;DR: Two methods that allow for the propagation of lines carrying dominant-negative transgenes in order to study protease-dead separase in embryos are developed and it is shown that prote enzyme- dead separase causes chromosome nondisjunction and cytokinesis failures.
References
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Journal ArticleDOI
Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
Paul T. Spellman,Gavin Sherlock,Gavin Sherlock,Michael Q. Zhang,Vishwanath R. Iyer,Kirk R. Anders,Michael B. Eisen,Patrick O. Brown,Patrick O. Brown,David Botstein,Bruce Futcher +10 more
TL;DR: A comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle is created, and it is found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins.
Journal ArticleDOI
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
TL;DR: Three chromosmal proteins that prevent premature separation of sister chromatids in yeast are described, two of which are members of the SMC family, which are putative ATPases with coiled-coil domains.
Journal ArticleDOI
Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
TL;DR: It is shown that Esp1 causes the dissociation of Scc1 from chromosomes by stimulating its cleavage by proteolysis, and a mutant SCC1 is described that is resistant to Esp1-dependent cleavage and which blocks both sister-chromatid separation and the dissociations from chromosomes.
Journal ArticleDOI
Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression
Journal ArticleDOI
The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae
TL;DR: It is shown that DNA replication also requires activation of Cdc28 by B-type (Clb) cyclins, and proteolysis of a cyclin-specific inhibitor of CDC28 is an essential aspect of the G1 to S phase transition.
Related Papers (5)
Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase.
Closing Mitosis: The Functions of the Cdc14 Phosphatase and Its Regulation
Frank Stegmeier,Angelika Amon +1 more