Mitotic Exit in the Absence of Separase Activity
Ying Lu,Frederick R. Cross +1 more
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TLDR
The first quantitative measure for Cdc14 release based on colocalization with the Net1 nucleolar anchor is defined, indicating efficient CDC14 release upon MEN activation; release driven by Esp1 in the absence of microtubules was inefficient and incapable of driving ME.Abstract:
In budding yeast, three interdigitated pathways regulate mitotic exit (ME): mitotic cyclin–cyclin-dependent kinase (Cdk) inactivation; the Cdc14 early anaphase release (FEAR) network, including a n...read more
Citations
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Journal ArticleDOI
Phosphatases: providing safe passage through mitotic exit
TL;DR: This work has shown that the key mitotic exit phosphatase in budding yeast, Cdc14, is now well understood, and in animal cells, it is now emerging that mitoticexit relies on distinct regulatory networks, including the protein phosphatases PP1 and PP2A.
Journal ArticleDOI
Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator.
Ying Lu,Frederick R. Cross +1 more
TL;DR: An intrinsically oscillatory module controlling nucleolar release and resequestration of the Cdc14 phosphatase is demonstrated, which is essential for mitotic exit in budding yeast and suggests that the intrinsically autonomous CDC14 release cycles are locked at once-per-cell-cycle through entrainment by the Cdk oscillator in wild-type cells.
Journal ArticleDOI
Inhibition of Cdc42 during mitotic exit is required for cytokinesis
Benjamin D. Atkins,Satoshi Yoshida,Satoshi Yoshida,Koji Saito,Chi-Fang Wu,Daniel J. Lew,David Pellman,David Pellman +7 more
TL;DR: A decrease in Cdc42 activation during mitotic exit is necessary to allow localization of key cytokinesis regulators and proper septum formation.
Journal ArticleDOI
Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes.
Joanna Bloom,Joanna Bloom,Ileana M. Cristea,Andrea L. Procko,Veronica Lubkov,Brian T. Chait,Michael Snyder,Frederick R. Cross +7 more
TL;DR: The findings suggest the dephosphorylation of the formins may be important for their observed localization change during exit from mitosis and indicate that Cdc14 targets proteins involved in wide-ranging mitotic events.
Journal ArticleDOI
From START to FINISH: computational analysis of cell cycle control in budding yeast.
TL;DR: This work crafted a new mathematical model of cell cycle progression in yeast that exploits a natural separation of time scales in the cell cycle control network to construct a system of differential-algebraic equations for protein synthesis and degradation, post-translational modifications, and rapid formation and dissociation of multimeric complexes.
References
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Journal ArticleDOI
A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae.
Kazuhide Asakawa,Akio Toh-e +1 more
TL;DR: The results suggest that a mutation in Kap104 stimulates exit from mitosis through the activation of Cdc14 and implies a novel role for Kap104 in cell-cycle progression in budding yeast.
Journal ArticleDOI
Cdc28-Clb mitotic kinase negatively regulates bud site assembly in the budding yeast.
C.G. Padmashree,Uttam Surana +1 more
TL;DR: It is suggested that the Cdc28-Clb kinase plays a critical role in the mechanism that restricts the timing of bud formation to the G(1) phase of the cell cycle.
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Separase, polo kinase, the kinetochore protein Slk19, and Spo12 function in a network that controls Cdc14 localization during early anaphase.
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